A double scanning microphotometer for image analysis: hardware, software and biomedical applications.

Brugal, G.; Garbay, Catherine; Giroud, Frederic; Adelh, D.

doi:10.1177/27.1.374569

Cited by 74 publications

(37 citation statements)

References 8 publications

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“…In invasive ductal carcinoma, the distribution of Bcl-2 protein is heterogeneous, involving not all the tumour cells and partly involving the cytoplasm adjacent to the cell membrane (Brugal et al, 1979). The two parameters of densitometric analysis, percentage of immunostained surface (vs counterstained surface) and mean optical density (MOD), which reflects the staining intensity (SAMBA arbitrary units scale ranging from 0 to 255), were obtained as previously reported (Charpin et al, 1988(Charpin et al, , 1995Charpin and Pellissier, 1994 …”

Section: Automated Immunohistochemistry Automated Immunohistochemistymentioning

confidence: 99%

Automated and quantitative immunocytochemical assays of Bcl-2 protein in breast carcinomas

Charpin

García²,

Bouvier³

et al. 1997

Br J Cancer

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Summary Expression of the bcl-2 gene was investigated in 218 human breast carcinomas by immunohistochemical analysis. Immunodetections were assessed using (1) frozen sections, (2) documented commercially available monoclonal antibody (bcl-2/124, Dako), (3) automation of immunoperoxidase technique (Ventana) and (4) quantitative evaluation of results by image analysis (SAMBA) and statistical analysis of quantitative data (BMDP software). Bcl-2 protein expression was correlated with current prognostic indicators and with molecular markers detected by the same procedure as for Bcl-2. It was shown that Bcl-2 expression is not related to patients' age, tumour size and type or lymph node status, but an inverse relationship was observed between Bcl-2 and tumour grade (P < 0.0001). An inverse relationship was also observed between Bcl-2 expression and p53 (P<0.0001), Ki67/MIB1 antigen-(P=0.0012), and P-gp-(P=0.002) positive immunoreactions. In contrast, anti-Bcl-2 positive reaction was significantly associated with ER-positive (P < 0.001) and with ER/PR-positive or ER/PR/pS2-positive immunoreactions (P < 0.005). Bcl-2 expression was independent of CD31 and cathepsin D expression. Thus, Bcl-2 protein, thought to be antiapoptotic, exhibits parodoxical expression in human breast carcinomas. It is strongly detected in low-grade tumours (well-differentiated) with low (MIB13) growth fraction, but is independent of the tumour progression (size, node status, CD31, and cathepsin D). Bcl-2 acting on apoptosis is related to p53 gene abnormalities in breast carcinomas. Bcl-2 protein expression may also be involved in response to endocrine therapy (associated to ER/PR/pS2 positive immunoreactions) and probably with chemoresistance mechanisms (inverse relationship with P-gp).

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Section: Automated Immunohistochemistry Automated Immunohistochemistymentioning

confidence: 99%

Automated and quantitative immunocytochemical assays of Bcl-2 protein in breast carcinomas

Charpin

García²,

Bouvier³

et al. 1997

Br J Cancer

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“…This lead to a wide expansion of instrument development for research and clinical applications. The first generations of DNA-ICM systems used for diagnostic purposes were SAMBA (Unilog, France), 37 LEYTAS (Leyden, Netherlands), 38 CAS (Becton Dickinson, San Jose, CA), 39 TAS-plus (Leitz, Wetzlar, Germany), 40 Roche Pathology Workstation (Roche Image Analysis), 7 AccuMed Cyto-Savant (AccuMed, Chicago, IL), 41 CYTOMETER CM-1 (Hund, Wetzlar, Germany), 42 and MIAMED-DNA (Leitz). 43 The appearance of a new generation of high-resolution video cameras and personal computers with high processing and storage capacities facilitated the development of high-performance DNA-ICM systems.…”

Section: Instrumentation and Softwarementioning

confidence: 99%

Diagnostic and prognostic use of DNA image cytometry in cervical squamous intraepithelial lesions and invasive carcinoma

Böcking

Nguyen

2003

Cancer

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In the fight against cervical malignancy and its precursors, several adjuvant diag- In patients with borderline lesions (mild and moderate dysplasias) of the uterine cervix, cytomorphology alone often is not sufficient for the early and definite cytologic detection of malignancy. Cervical dysplasia describes squamous cells or tissues that potentially may lead to malignancy but do not exhibit sufficient evidence for a definite assumption. Resulting from weakness of morphologic criteria to identify malignant transformation in epithelial cells early and unequivocally, dysplasias are not a disease entity. The widely accepted assumption is that the higher the grade of dysplasia is, the higher the probability of progression to carcinoma is.1 However, as a result of insufficient morphologic criteria, neither histologic nor cytologic evaluation can predict whether a lesion will progress to cancer in an individual patient.2 Rates of regression and progression of cervical dysplasia (positive and negative predictive values) are quite different from one study to another. 1 Insufficient interobserver reproducibility in diagnostic cytology and histology represents another dilemma in the microscopic diagnosis of precancerous cervical intraepithelial lesions.Because the diagnosis of cervical squamous intraepithelial lesions (SILs) is not only poorly reproducible but also of limited biologic meaning for the individual patient, the number of resulting control procedures usually is high. These range from repeated cytologic smears and biopsies to unnecessary operations (conizations). Missed early diagnoses of cancers may result from cytomorphologic uncertainties. This also results in unnecessary costs and avoidable anxiety for the patients.In the late 1970s, zur Hausen suggested that there may be an association between human papillomavirus (HPV) and cervical carcinoma.3 Large numbers of subsequent epidemiologic, clinicopathologic, and molecular studies have linked the presence of specific types of HPV to the development of anogenital carcinoma and its precursors. A recent study estimated the worldwide HPV prevalence in cervical carcinomas at 99.7%. 4 Today, it is widely accepted that HPVs play a critical role in the pathogenesis of most cervical carcinomas and their precursor lesions. The infection of cervical epithelial cells with HPV itself is necessary but still insufficient for neoplastic progression. Other factors, especially genetic alterations, are needed to enter into the process of neoplastic transformation. 5Several adjuvant diagnostic methods currently are proposed to increase the diagnostic accuracy and reproducibility of cytology and histology. These range from clinical procedures, like colposcopy, to laboratory methods, like assays for the detection of HPV DNA and HPV typing 6 or DNA image cytometry (DNA-ICM). 1,7,8 In the current review, we focus on the technical performance and application of DNA-ICM in diagnosis and prognosis of cervical SILs and invasive cervical carcinoma. Biologic BackgroundChromosomal aneuploi...

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“…Immunostaining was analysed using an Axiophot microscope (Zeiss) and a 3 CCD Sony camera and then processed in an image analysis device (SAMBA 2005, Alcatel TITN, Grenoble, France) breast carcinomas, evaluated by automated (Ventana) immunoperoxidase on frozen sections: quantitation by computer-assisted analysis (SAMBA) of digitized coloured microscopic images (ordinate, % of patients; abscissa, % of immunostained surface vs counterstained surfaces) (Brugal et al, 1979). The two parameters of the densitometric analysis, the percentages of positive immunostained surfaces (membrane and cytoplasm) vs counterstained surface and mean optical density (MOD), which depends upon the staining intensity (SAMBA arbitrary units scale: 0-255), were obtained as reported previously (Charpin et al, 1988(Charpin et al, , 1992(Charpin et al, , 1993(Charpin et al, , 1994(Charpin et al, , 1995a.…”

Section: Image Analysismentioning

confidence: 99%

c-erbB-2 oncoprotein detected by automated quantitative immunocytochemistry in breast carcinomas correlates with patients' overall and disease-free survival

Charpin

García²,

Bouvier³

et al. 1997

Br J Cancer

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Summary The prognostic significance of c-erbB-2 oncoprotein overexpression detected in tumours by immunocytochemical assays (ICAs) was investigated in 148 breast carcinomas. ICAs were performed under optimal technical conditions with frozen tissue sections and included automated immunoperoxidase technique and computer-assisted analysis (densitometry) of digitized coloured microscopic images. Results of quantitative ICAs (expressed in percentages of c-erbB-2-positive surfaces and mean optical densities) were correlated with the patients' follow-up in axillary lymph node-positive (N+) and node-negative (N-) subgroups of patients. Patients' follow-up ranged from 9 months (for the first death) to 101 months (for the 121 alive patients) with a 62.5 months mean overall follow-up. It was shown that marked c-erbB-2 immunocytochemical expression in tumours (cut-off point 35%) significantly correlated with the patients' poor overall survival in N+ and in N patients (Kaplan-Meier, log-rank test, P = 0.045 and P = 0.015). Also, marked c-erbB-2 immunohistochemical expression correlates with short disease-free (P = 0.005), recurrence-free (P = 0.048) and metastasis-free survival (P = 0.05) (Kaplan-Meier, log-rank test) in N+, but not in N-subgroups. It is concluded that in optimal conditions (automated and quantitative ICAs on frozen sections) c-erbB immunohistochemical expression is a significant prognostic indicator in terms of overall and disease-free survival. The c-erbB-2 protein prognostic significance is independent of node status in terms of overall survival, but not of disease-free survival.

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A double scanning microphotometer for image analysis: hardware, software and biomedical applications.

Cited by 74 publications

References 8 publications

Automated and quantitative immunocytochemical assays of Bcl-2 protein in breast carcinomas

Automated and quantitative immunocytochemical assays of Bcl-2 protein in breast carcinomas

Diagnostic and prognostic use of DNA image cytometry in cervical squamous intraepithelial lesions and invasive carcinoma

c-erbB-2 oncoprotein detected by automated quantitative immunocytochemistry in breast carcinomas correlates with patients' overall and disease-free survival

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