A double-edged sword: The Kelch-like ECH-associated protein 1-nuclear factor erythroid-derived 2-related factor 2-antioxidant response element pathway targeted pharmacological modulation in nonalcoholic fatty liver disease

LipidSIM: Inferring mechanistic lipid biosynthesis perturbations from lipidomics with a flexible, low-parameter, Markov modeling framework

“…We demonstrate the value of this framework LipidSIM with three case studies. First, we analyzed hepatic lipidomic data (Keap1 dataset) from our own Gubra-Amylin NASH (GAN) dietfed murine models treated with antisense oligonucleotides (ASO) targeting Keap1, a key regulator of cellular oxidative stress implicated in NASH progression (29)(30)(31). Through this application, we systematically characterized the perturbed lipidome at different resolutions to generate testable hypotheses regarding lipid biosynthesis reactions.…”