A Double-blind Trial of Lactobacillus paracasei Strain KW3110 Administration for Immunomodulation in Patients with Pollen Allergy

Inhibitory Effect ofLactobacillus gasseriTMC0356 andLactobacillusGG on Enhanced Vascular Permeability of Nasal Mucosa in Experimental Allergic Rhinitis of Rats

“…These results are in good agreement with previous human studies with Lactobacillus GG and other probiotic strains, in which these strains significantly improved the symptoms of allergic diseases but did not greatly alter serum IgE. 5,[7][8][9] Suplatast tosilate (IPD-1151T) is an antiallergic agent that inhibits T-cell synthesis of IL-4 and IL-5 in both human and murine Th2 cells. 22) IPD-1151T also inhibits the growth of mast cells and the release of chemical medicates from mast cells.…”

“…3,4) Recently, selected probiotic strains altered allergic disease in several well-designed clinical studies while they persisted in the human intestinal epithelium. [5][6][7][8][9] However, the underlying mechanisms by which probiotic strains can influence allergic disease remain unclear. The present study aimed to find additional evidence to support the effect of probiotic lactic acid bacteria on allergic rhinitis in an experimental animal model.…”

Characterization of Candidate Anti-Allergic Probiotic Strains in a Model of Th2-Skewed Human Peripheral Blood Mononuclear Cells

“…Collectively, these findings suggest that induction of mild inflammation and particularly of IFN-γ secretion could be characteristic of a protective anti-allergic response, a hypothesis that has been put forward previously [38]. In our present study, we included strains which had proven anti-allergic activity in vivo either in human or in pre-clinical animal studies, for example L. rhamnosus NCC 4007 [2], which is indistinguishable by molecular typing from L. rhamnosus ATCC 53103, L. paracasei NCC 4014 which is equivalent to strain KW3110 [25], B. longum NCC 3001 which corresponds to strain BB536 [15], or L. paracasei NCC 2461 who was recently shown to reduce symptoms of allergic rhinitis [39]. Interestingly, most of these strains shared a ‘discrete’ cytokine profile, with low IFN-γ and IL-10 but efficient inhibition of IL-5 production (table 1, fig.…”

“…Fujiwara et al [24] partly overcame these limitations by using Th2-skewed splenocytes from ovalbumin (OVA)-primed mice. Notably, a strain of Lactobacillus paracasei selected on the basis of its ability to induce IL-12 and inhibit IL-4 secretion in this model showed a benefit in a subsequent clinical trial in humans suffering from allergic rhinitis [25]. Alternatively, Vissers et al [26] recently showed that co-culture of specific probiotic strains with PBMC from human allergic donors decreased IL-13 production along with strain-specific effects on induction of Th1 cytokines and cell surface markers.…”

Evaluation of the probiotic potential of Lactobacillus paracasei KW3110 based on in vitro tests and oral administration tests in healthy adults

“…Oral administration of L. paracasei KW3110 to ovalbumin-sensitized BALB/c mice resulted in induction of IL-12 secretion as a Th1 parameter and repression of IL-4 secretion as a Th2 parameter from splenocytes, and suppression of serum IgE elevation, compared with control animals that did not receive the cells of L. paracasei KW3110 (Fujiwara et al, 2004). In addition, administration of L. paracasei KW3110 to patients with allergic symptoms to cedar pollen appeared to improve their Th1/Th2 balance and suppress ECP elevation (Fujiwara et al, 2005). Furthermore, it has been suggested that oral intake of L. paracasei KW3110 suppressed the symptoms of dermatitis and production of IgE in atopic dermatitis model animals induced by applying picryl chloride onto the skin of NC/Ng mice (Wakabayashi et al, 2007).…”