A double blind, randomised, multicentre comparison of two doses of intravenous iloprost in the treatment of Raynaud's phenomenon secondary to connective tissue diseases.

Torley, H. I.; Madhok, Rajan; Capell, Hilary A.; Brouwer, R.; Maddison, P. J.; Black, Carol M.; Englert, H.; Dormandy, J. A.; Watson, H.R.

doi:10.1136/ard.50.11.800

Cited by 71 publications

(39 citation statements)

References 24 publications

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“…Tcell clones were made as described previously [14, [18], deletion mutants of the M . bovis BCG hsp65 [12] and peptides synthesized according to the sequence of the M .…”

Section: Methodsmentioning

confidence: 99%

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Dr3-restricted t cells from different hla-dr3-positive individuals recognize the same peptide (amino acids 2–12) of the mycobacterial 65-kda heat-shock protein

Schooten¹,

Elferink²,

Embden³

et al. 1989

Eur. J. Immunol.

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Studies in experimental animals have demonstrated that the T cell response to immunogenic proteins is limited to one or a few epitopes on such proteins and that the MHC haplotype of the responder is an important factor in determining which epitope is recognized (immune response gene effect). However, if and to what extent MHC genes control the immune response to pathogens in man is virtually unknown. We have studied the human T cell response to the mycobacterial 65-kDa heat-shock protein, a major immunogen of Mycobacterium leprae and M. tuberculosis, the causative agents of leprosy and tuberculosis, respectively, in relation to HLA-DR phenotype. In a large panel of short-term cultured polyclonal anti-mycobacterial T cell lines, from 45 different individuals representing all DR-restriction specificities, only DR1 and DR3-restricted T cell lines proliferated to the 65-kDa protein. The DR1-restricted T cell lines responded to three new epitopes on the mycobacterial 65-kDa protein, one of which is specific for the M. tuberculosis complex. Altogether nine T cell epitope-containing regions have now been mapped on the 65-kDa protein and the response to each of them was exclusively restricted via one HLA-DR allele. Most importantly, all six 65-kDa-responsive DR3-restricted T cell lines from different individuals recognized an epitope on the same peptide, representing amino acids 2-12 of the 65-kDa protein, that was previously mapped using DR3-restricted T cell clones. From these data we conclude that the human T cell response to both the whole mycobacterial 65-kDa heat-shock protein and to defined epitopes on this protein is controlled by HLA-DR genes. The mycobacterial 65-kDa protein has been implicated in the design of subunit vaccines against tuberculosis and leprosy as well as the induction of immunopathology. In both instances the Ir gene control of the T cell response to this protein may have to be taken into account.

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“…Tcell clones were made as described previously [14, [18], deletion mutants of the M . bovis BCG hsp65 [12] and peptides synthesized according to the sequence of the M .…”

Section: Methodsmentioning

confidence: 99%

“…All Tcell lines were tested in a proliferation assay with a 65-kDa protein of M . bovis [18] and M . leprue [17] DR1 and six via DR3.…”

Section: Hsp 65 Reactivity and Hla-dr Restriction Specificity Of Antimentioning

confidence: 99%

Dr3-restricted t cells from different hla-dr3-positive individuals recognize the same peptide (amino acids 2–12) of the mycobacterial 65-kda heat-shock protein

Schooten¹,

Elferink²,

Embden³

et al. 1989

Eur. J. Immunol.

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“…It has been shown to reduce the severity, frequency and duration of vasospastic events and promote healing of ischaemic ulcers. [4][5][6] There have been several, long-term, randomised trials on prostacyclin analogues versus placebo patients with Fontaine's stages III and IV; 6,7 these have shown a beneficial effect. The recommendation from the Trans-Atlantic Inter-Society Consensus (TASC) is that the use of prostanoids in end-stage critical limb ischaemia maybe of use in preventing further tissue loss or amputation.…”

Section: Discussionmentioning

confidence: 99%

Digital artery occlusion secondary to plastic shopping bag trauma

Joy

Isaacs²,

McCarthy³

2007

Ann R Coll Surg Engl

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Upper limb digital arterial occlusion is uncommon. We present the case of a 47-year-old man with an ischaemic right middle finger (dominant hand) due to trauma from carrying a heavy plastic shopping bag. Duplex scanning revealed no proximal source of emboli. An angiogram demonstrated occlusions in the medial and lateral digital arteries of the middle digit at the level of the proximal phalanx. Treatment with heparin and warfarin resulted in complete resolution of symptoms.

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“…hsp 60 for immunization was produced, isolated and purified from E. coli clone M1546 [9] essentially as described earlier [ 101 .The hyperimmune polyclonal rabbit IgG fraction (rb-a-60) was prepared by affinity purification on a protein A-Superose (Pharmacia, Freiburg, FRG) column and titrated for saturating concentration on BMMQ cells by FCM. As a negative control we used a normal rabbit IgG preparation which had been absorbed on an hsp 60 affinity column (CNBr-Sepharose coupled with purified rhsp 60 of M. bovis BCG following the instruction of Pharmacia) to remove contaminating naturally occurring anti-hsp 60 antibodies.…”

Section: Antisera and Mabmentioning

confidence: 99%

Surface expression by mononuclear phagocytes of an epitope shared with mycobacterial heat shock protein 60

et al. 1991

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Bone marrow-derived macrophages were stained with a variety of monoclonal antibodies (mAb) with specificity for the mycobacterial heat shock protein (hsp) 60 or with specific rabbit antiserum raised against mycobacterial hsp 60. The mAb ML30 as well as the specific antiserum brightly stained bone marrow-derived macrophages whereas all the other mAb as well as normal rabbit antiserum gave no or negligible reactivity. Surface expression of the shared epitope is observed by day 3 of in vitro cultivation and markedly increased by interferon-gamma stimulation on day 9. Although hsp 60 is thought to be restricted to the mitochondrial compartment, antibody responses to this molecule have been implicated in autoimmunity. Our finding that bone marrow-derived macrophages express a cross-reactive epitope recognized by an mAb with specificity for amino acids 275 to 295 of the mycobacterial hsp 60 is consistent with such a role.

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A double blind, randomised, multicentre comparison of two doses of intravenous iloprost in the treatment of Raynaud's phenomenon secondary to connective tissue diseases.

Abstract: Objective-To compare low (0.5 ng/kg/min) and standard dose (2 ng/kg/min) iloprost (a stable carbacyclin analogue of prostacyclin) in patients with Raynaud's phenomenon secondary to connective tissue disorders.

Cited by 71 publications

References 24 publications

Dr3-restricted t cells from different hla-dr3-positive individuals recognize the same peptide (amino acids 2–12) of the mycobacterial 65-kda heat-shock protein

Dr3-restricted t cells from different hla-dr3-positive individuals recognize the same peptide (amino acids 2–12) of the mycobacterial 65-kda heat-shock protein

Digital artery occlusion secondary to plastic shopping bag trauma

Surface expression by mononuclear phagocytes of an epitope shared with mycobacterial heat shock protein 60

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