A Double-Blind Controlled Study to Evaluate the Effects of Yogurt Enriched with Lactococcus lactis 11/19-B1 and Bifidobacterium lactis on Serum Low-Density Lipoprotein Level and Antigen-Specific Interferon-γ Releasing Ability

Abstract

In order to clarify the effects of the Lactococcus lactis (L. lactis) 11/19-B1 strain, a double-blind controlled study of yogurt fermented with the strain was carried out. For the study, two kinds of yogurt, the control and test yogurt, were prepared; the control yogurt was fermented with Streptococcus thermophiles, Lactobacillus delbrueckii subspecies bulgaricus, and Lactobacillus acidophilus, and the test yogurt was enriched with L. lactis 11/19-B1 and Bifidobacterium lactis (B. lactis) BB-12 strains. Seventy-six volunteers who had not received treatment with pharmaceuticals were randomly divided into two groups with each group ingesting 80 g of either the test or control yogurt every day for 8 weeks. Before and after yogurt intake, fasting blood was taken and blood sugar, blood lipids, and anti-cytomegalovirus cellular immunity were estimated. In the test yogurt group, low-density lipoprotein (LDL) was significantly decreased (159.1 ± 25.7 to 149.3 ± 24.4; p = 0.02), but this effect was not observed in the control yogurt group. When the test yogurt group was divided into two groups based on LDL levels of over or under 120 mg/dL, this effect was only observed in the high LDL group. No LDL-lowering effect of B. lactis BB-12 strain was previously reported; therefore, the hypocholesterolemic effects observed in this study are thought to be caused by the L. lactis 11/19-B1 strain alone or its combination with the B. lactis BB-12 strain.

The Effect of Probiotics and Synbiotics on Risk Factors Associated with Cardiometabolic Diseases in Healthy People—A Systematic Review and Meta-Analysis with Meta-Regression of Randomized Controlled Trials

“…The mean probiotic administration was 67.01 ± 38.72 days (range = 28–186 days). Probiotic, not synbiotic, interventions were predominantly conducted ( n = 54) [ 29 , 30 , 31 , 32 , 33 , 34 , 35 , 36 , 39 , 40 , 41 , 42 , 43 , 44 , 46 , 47 , 49 , 50 , 51 , 52 , 53 , 54 , 55 , 56 , 57 , 58 , 59 , 60 , 61 , 62 , 63 , 64 , 66 , 67 , 68 , 69 , 71 , 72 , 73 , 74 , 75 , 76 , 77 , 78 , 79 , 80 , 81 , 82 , 83 , 84 , 85 , 86 , 88 , 89 ]. Probiotic powders were administered in 15 studies [ 31 , 36 , ...…”

“…The daily doses varied between 10 6 and 10 10 CFU (colony-forming units). The trials were financed by only industry budgets in 20 ( n = 20) [ 29 , 30 , 33 , 34 , 39 , 41 , 42 , 46 , 47 , 48 , 55 , 64 , 67 , 69 , 75 , 76 , 78 , 79 , 83 , 84 ]. Studies were financed only by academic resources in 10 studies ( n = 10) [ 31 , 32 , 35 , 37 , 40 , 43 , 44 , 52 , 58 , 60 ].…”

The Efficacy of Short-Term Weight Loss Programs and Consumption of Natural Probiotic Bryndza Cheese on Gut Microbiota Composition in Women

“…This genus does not gain too much interest for its probiotic activity because the main attention is focused on the other LAB genera as Lactobacillus and Bifidobacterium [ 63 ]. However, some studies have shown the beneficial effects of products fermented with Lactococcus in the gut [ 64 ]. A previous study on subjects with slightly elevated blood lipid or blood sugar levels demonstrated that the administration of yogurt fermented with the Lactococcus lactis 11/19-B1 strain for 8 weeks significantly reduced total cholesterol, LDL and LDL/HDL ratio [ 64 ].…”

“…However, some studies have shown the beneficial effects of products fermented with Lactococcus in the gut [ 64 ]. A previous study on subjects with slightly elevated blood lipid or blood sugar levels demonstrated that the administration of yogurt fermented with the Lactococcus lactis 11/19-B1 strain for 8 weeks significantly reduced total cholesterol, LDL and LDL/HDL ratio [ 64 ]. Moreover, 8 weeks of drinking skim milk fermented with Lactococcus genera has shown a positive impact on the systolic blood pressure level in prehypertensive individuals [ 65 ].…”

Draft Genome Sequence of the Lactococcus lactis 11/19-B1 Strain, Isolated from Kiwifruit

“…It is reported that the L. lactis 11/19-B1 strain, isolated from the surface of kiwifruit, markedly stimulates innate immunity in silkworms and shows some differences in sugar utilization and enzymatic characteristics in comparison with other L. lactis strains ( 1 ). Furthermore, we reported that the intake of yogurt containing 11/19-B1 significantly decreased the level of low-density lipoproteins (LDLs) in high-LDL volunteers in comparison to that of the control yogurt ( 2 ). Moreover, 11/19-B1 improved atopic dermatitis (AD) in humans and AD-related dorsal skin and ear lesions in a 1-fluoro-2,4-dinitrobenzene-induced AD mouse model ( 3 ).…”