A dose-response relationship between exposure to cockroach allergens and induction of sensitization in an experimental asthma in Hartley guinea pigs☆☆☆★★★

Zhou, Daohong; Chen, Gang; Kim, Jin-Tai; Lee, Lu-Yuan; Kang, Bann C.

doi:10.1016/s0091-6749(98)70174-5

Cited by 19 publications

(16 citation statements)

References 26 publications

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“…Thus, it is suggested that cockroach allergens in the HDE play the major role in pulmonary eosinophilia and AHR observed in this mouse model of asthma. The allergen-specific pulmonary infiltration of eosinophils in this model is very similar to the asthmatic responses demonstrated in the aerosolized whole-body extract of cockroach-sensitized guinea pig models (21) and in a mouse model using purified cockroach allergen (35). The cockroach-sensitized guinea pigs showed a dose-response relationship between the number of eosinophils recruited in the BAL and the amount of cockroach allergen.…”

Section: Discussionsupporting

confidence: 68%

“…Our results are similar to other studies regarding the kinetics of eosinophil recruitment where pulmonary eosinophilia peaked at 48 h after the last allergen challenge in cockroach allergen-sensitized mouse model (35). Pulmonary eosinophilia peaked 3 h after the last challenge in an OVA-sensitized and -challenged mouse model (15) and peaked in 24 h after the last challenge in a cockroach whole body extract-sensitized and -challenged guinea pig model (21).…”

Section: Discussionmentioning

confidence: 99%

“…In an effort to develop allergic asthma-like pulmonary inflammation in healthy animals, mice were sensitized and/or challenged with various kinds of allergens. Among them, OVA (20) and purified indoor allergens such as cockroach (21) and dust mite (22) are commonly used allergens for murine models. However, the allergens used for these animal models may not represent the same constituents to which asthmatics are exposed throughout their daily life in terms of quality and quantity of allergens.…”

mentioning

confidence: 99%

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Eotaxin Represents the Principal Eosinophil Chemoattractant in a Novel Murine Asthma Model Induced by House Dust Containing Cockroach Allergens

Kim

Merry

Nemzek

et al. 2001

The Journal of Immunology

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Asthma represents a serious health problem particularly for inner city children, and recent studies have identified that cockroach allergens trigger many of these asthmatic attacks. This study tested the concept that asthma-like pulmonary inflammation may be induced by house dust containing cockroach allergens. An aqueous extract was prepared from a house dust sample containing endotoxin and high levels of cockroach allergens. BALB/c mice were immunized with the house dust extract (HDE) and received two additional pulmonary challenges. Bronchoalveolar lavage (BAL) eosinophil counts and eotaxin levels were significantly increased in immunized mice exposed to the HDE, whereas neutrophils were the predominant BAL inflammatory cell in the unimmunized mice. Kinetics studies in immunized mice demonstrated a peak pulmonary inflammatory response 48 h after the last challenge. The allergic response in this model was further confirmed by histological and physiological studies demonstrating a significant influx of eosinophils and lymphocytes in the peribronchial area, and severe airway hyperreactivity through whole-body plethysmography. The specificity of the response was established by immunizing with HDE and challenging with purified cockroach allergen, which induced pulmonary eosinophilia and airway hyperreactivity. Ab inhibition of eotaxin significantly inhibited the number of BAL eosinophils. These data describe a novel murine model of asthma-like pulmonary inflammation induced by house dust containing endotoxin and cockroach allergens and further demonstrate that eotaxin represents the principal chemoattractant for the recruitment of the pulmonary eosinophils.

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Section: Discussionsupporting

confidence: 68%

Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

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Eotaxin Represents the Principal Eosinophil Chemoattractant in a Novel Murine Asthma Model Induced by House Dust Containing Cockroach Allergens

Kim

Merry

Nemzek

et al. 2001

The Journal of Immunology

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“…In a murine model of asthma [39] using 6- to 8-week-old female animals, eosinophil infiltration, airway hyperresponsiveness and IgE production decreased as the sensitization dose of OVA increased. Studies in the guinea pig over a limited dosing range of cockroach allergen demonstrated a relationship between the dose of allergen and antibody production, but the severity of asthma as measured by bronchoconstriction was not dose related over the same allergen range [40]. …”

Section: Discussionmentioning

confidence: 99%

Primary Prevention of Asthma: Age and Sex Influence Sensitivity to Allergen-Induced Airway Inflammation and Contribute to Asthma Heterogeneity in Guinea Pigs

Regal¹,

Regal

Meehan³

et al. 2006

Int Arch Allergy Immunol

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Background: Limiting allergen exposure in the sensitization phase has been proposed as a means of primary prevention of asthma, but its effectiveness is debated. Hypothesis: Primary prevention of asthma is more effective in limiting asthma symptoms in young guinea pigs compared with adults, whether males or females. Methods: The following experimental groups were used: young/young, sensitized and challenged before sexual maturity; young/adult, sensitized young and challenged after sexual maturity; adult/adult, sensitized and challenged after sexual maturity. Males and females were sensitized intraperitoneally with varying doses of ovalbumin (OVA) and challenged intratracheally with a constant OVA dose. Cellular infiltration into lung and lavage fluid as well as airway hyperresponsiveness to intravenous methacholine was determined 24 h later. Results: In unsensitized animals, density of resident inflammatory cells as well as baseline pulmonary function differed with age and sex. Maximum OVA-induced eosinophilia in females occurred at a lower sensitizing dose of OVA than in males, and the slopes of the dose-response relationship differed significantly between sexes. Young females had more pronounced increases in eosinophils compared with some adult treatment groups. The concentrations of OVA-specific antibodies were not directly related to differences in cellular infiltration. Airway hyperresponsiveness to methacholine challenge was observed in all treatment groups. Conclusion: Young animals require major reductions in allergen exposure compared with adults to effectively limit airway inflammation in primary prevention. Heterogeneity of asthma symptoms seen with age and sex suggests that primary prevention by limiting allergen exposure or treatment with anti-inflammatory or bronchodilator drugs may be more effective strategies for specific age and gender populations.

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“…In normal healthy animals, it is necessary to immunize and challenge the mice with specific antigens (Zhou et al, 1998;Campbell et al, 1999;Clarke et al, 1999;Tournoy et al, 2000) to induce allergic asthma-like pulmonary inflammation. These types of animal models have helped to establish asthma as a unique form of chronic airway inflammation involving lymphocytes, mast cells, and eosinophils.…”

mentioning

confidence: 99%

Noninvasive Quantitative Tomography of the Therapeutic Response to Dexamethasone in Ovalbumin-Induced Murine Asthma

Korideck¹,

Peterson²

2009

J Pharmacol Exp Ther

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Animal models of pulmonary inflammation are critical for understanding the pathophysiology of asthma and for developing new therapies. Current conventional assessments in mouse models of asthma and chronic obstructive pulmonary disease rely on invasive measures of pulmonary function and terminal characterization of cells infiltrating into the lung. The ability to noninvasively visualize and quantify the underlying biological processes in mouse pulmonary models in vivo would provide a significant advance in characterizing disease processes and the effects of therapeutics. We report the utility of near-infrared imaging agents, in combination with fluorescence molecular tomography (FMT) imaging, for the noninvasive quantitative imaging of mouse lung inflammation in an ovalbumin (OVA)-induced chronic asthma model. BALB/c mice were intraperitoneally sensitized with OVA-Alum (aluminum hydroxide) at days 0 and 14, followed by daily intranasal challenge with OVA in phosphate-buffered saline from days 21 to 24. Dexamethasone and control therapies were given intraperitoneally 4 h before each intranasal inhalation of OVA from days 21 to 24. Twentyfour hours before imaging, the mice were injected intravenously with 5 nmol of the cathepsin-activatable fluorescent agent, ProSense 680. Quantification by FMT revealed in vivo cysteine protease activity within the lung associated with the inflammatory eosinophilia, which decreased in response to dexamethasone treatment. Results were correlated with in vitro laboratory tests (bronchoalveolar lavage cell analysis and immunohistochemistry) and revealed good correlation between these measures and quantification of ProSense 680 activation. We have demonstrated the ability of FMT to noninvasively visualize and quantify inflammation in the lung and monitor therapeutic efficacy in vivo.

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A dose-response relationship between exposure to cockroach allergens and induction of sensitization in an experimental asthma in Hartley guinea pigs☆☆☆★★★

Cited by 19 publications

References 26 publications

Eotaxin Represents the Principal Eosinophil Chemoattractant in a Novel Murine Asthma Model Induced by House Dust Containing Cockroach Allergens

Eotaxin Represents the Principal Eosinophil Chemoattractant in a Novel Murine Asthma Model Induced by House Dust Containing Cockroach Allergens

Primary Prevention of Asthma: Age and Sex Influence Sensitivity to Allergen-Induced Airway Inflammation and Contribute to Asthma Heterogeneity in Guinea Pigs

Noninvasive Quantitative Tomography of the Therapeutic Response to Dexamethasone in Ovalbumin-Induced Murine Asthma

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