A dopamine transporter in human erythrocytes: modulation by insulin

Azoui, Rachida; Jl, Cuche; Renaud, Jean‐François; Safar, Michel E.; Dagher, G.

doi:10.1113/expphysiol.1996.sp003946

Cited by 16 publications

(11 citation statements)

References 24 publications

(46 reference statements)

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“…Catecholamines co‐incubated with PRBCs demonstrate measured plasma levels greater than would be predicted if distributed throughout the entire intra‐ and extra‐cellular space but less than would be predicted if confined to the extracellular plasma volume. RBCs contain transporters which allow for uptake of dopamine, norepinephrine, and epinephrine . The activity of these transport systems depend upon the fasting state of the individual as well as factors such as available insulin .…”

Section: Discussionmentioning

confidence: 99%

Effect of incubation with crystalloid solutions or medications on packed red blood cells

et al. 2019

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BACKGROUND American Association of Blood Banks (AABB) guidelines suggest that packed red blood cells (PRBCs) be administered through a dedicated intravenous (IV) catheter. Literature supporting this broad‐scope declaration are scarce. Obtaining additional IV access is painful, costly, and an infectious risk. We evaluated the effect of co‐incubating PRBCs with crystalloids and medications on PRBC hemolysis, membrane deformability, and aggregation, as well as medication concentration. METHODS PRBCs were co‐incubated 5 minutes with plasma, normal saline (NS), 5% dextrose in water (D5W), Plasmalyte, epinephrine (epi), norepinephrine (norepi), dopamine (dopa), or Propofol (prop). Samples were then assessed for hemolysis (free hemoglobin, serum potassium), membrane deformability (elongation index [EI]), aggregation (smear, critical shear stress [mPa]) and drug concentration (High Performance Liquid Chromatography/Tandem Mass Spectrometry [LCMS‐MS]). Significance (p ≤ 0.05) was determined by Wilcoxon‐paired comparisons or Wilcoxon/Kruskall Willis with post‐hoc Dunn's test. RESULTS Compared to co‐incubation with plasma: 1) co‐incubation resulted in significantly increased hemolysis only when D5W as used (free hemoglobin, increased potassium); 2) EI trended lower when co‐incubated with D5W and trended toward higher when co‐incubated with prop; 3) aggregation was significantly lower when PRBCs co‐incubated with NS, D5W, or Plasmalyte, and trended lower when co‐incubated with epi, norepi, or dopa. Medication concentrations were between those predicted by distribution only in plasma and distribution through the entire intra‐ and extracellular space. CONCLUSION Our data suggest that 5 minutes of PRBC incubation with isotonic crystalloids or catecholamines does not deleteriously alter PRBC hemolysis, membrane deformability, or aggregation. Co‐incubation with D5W likely increases hemolysis. Propofol may promote hemolysis.

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Section: Discussionmentioning

confidence: 99%

Effect of incubation with crystalloid solutions or medications on packed red blood cells

et al. 2019

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“…There is a biphasic decay in plasma catecholamine levels, the first phase lasting around 5 min (uptake‐2), and the second occurring over a month (plasma protein decay) (El‐Bahr et al ., 2006). Catecholamine metabolism occurs slowly in erythrocytes, which also act as a storage pool (Azoui et al ., 1996). Uptake‐1 and 2 account for 25% of adrenaline clearance from plasma (Clutter et al ., 1980).…”

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confidence: 99%

Use of inotropes and vasopressor agents in critically ill patients

Bangash

Kong

Pearse

2012

British J Pharmacology

163

138

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Inotropes and vasopressors are biologically and clinically important compounds that originate from different pharmacological groups and act at some of the most fundamental receptor and signal transduction systems in the body. More than 20 such agents are in common clinical use, yet few reviews of their pharmacology exist outside of physiology and pharmacology textbooks. Despite widespread use in critically ill patients, understanding of the clinical effects of these drugs in pathological states is poor. The purpose of this article is to describe the pharmacology and clinical applications of inotropic and vasopressor agents in critically ill patients. LINKED ARTICLESThis article is commented on by Bracht et al., pp. 2009 and De Backer and Scolletta, pp. 2012-2014 ]i, Intracellular calcium concentration; ATP, adenosine triphosphate; cAMP, cyclic adenosine monophosphate; CPR, cardiopulmonary resuscitation; DO2, systemic oxygen delivery; GPCR, G-protein coupled receptor; GTP/GDP, guanosine triphosphate/diphosphate; PKA/PKC, protein kinase A/C; PLC, phospholipase C; SvO2, mixed/central venous oxygen saturation; TNF-a, tumour necrosis factor alpha; VO2, oxygen consumption IntroductionInotropes are agents administered to increase myocardial contractility whereas vasopressor agents are administered to increase vascular tone. The use of these potent agents is largely confined to critically ill patients with profound haemodynamic impairment such that tissue blood flow is not sufficient to meet metabolic requirements. Examples include patients with severe heart failure and septic or cardiogenic shock, as well as patients undergoing major surgery and victims of major trauma. They are generally administered via a large central vein and, in some specific situations, via a peripheral vein. These agents have a diverse range of actions including metabolic and immune effects, many of which are poorly understood. The objective of this review is to describe the underlying cardiovascular mechanisms that clinicians seek to influence through the use of inotropic agents, to describe the basic pharmacology of those drugs in common use and, finally, to explore the evidence base for specific approaches to inotrope and vasopressor therapy in clinical practice. As many of the commonly used agents exert both inotropic and vasopressor effects, the term 'inotrope' will be generally used in this review to describe agents with a spectrum of actions. The physiological basis for the actions of inotropic agentsMyocyte excitation and contraction. Cardiac muscle fibres contract through the sliding filament mechanism. Actin and myosin filaments are propelled past each other through repeated cross-bridge linking and unlinking. Each cardiac action potential results in the opening of voltage-gated myocyte calcium channels and a rise in intracellular calcium concentration ([Ca 2+ ]i). This triggers a further release of calcium from the sarcoplasmic reticulum, which accounts for around three quarters of the total increase in [Ca 2+ ]i (Levick, 2003...

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“…As mentioned previously, 41% of CAs was bound (about 30% to the erythrocytes and about 11% to plasma proteins) and the other 59% might be either unbound or partly bound to unknown binding systems (such as small peptides). The fact that the radioactivity was also found within the red blood cells suggests a transport system through the membrane as described for human erythrocytes (Azoui et al, 1996). The radioactivity may be bound to haemoglobin (`catecholaminated' haemoglobin) by the same mechanism described for glucose (glycosylated haemoglobin; Khaw et al, 2001).…”

Section: Discussionmentioning

confidence: 96%

Binding and Clearance of Radioactive Adrenaline and Noradrenaline in Sheep Blood

Kahlbacher²,

Patzl

et al. 2006

Vet Res Commun

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An understanding of the conditions influencing protein binding of catecholamines (CAs) is important in studying their metabolic effects. Unfortunately, reports on plasma protein binding of CAs are scarce, conflicting and mainly performed in vitro. The aim of our in vivo and in vitro studies was to investigate binding and clearance of radioactive adrenaline (epinephrine) ((3)H-A), noradrenaline (norepinephrine) ((3)H-NA) and their metabolites in sheep blood. The time course of the radioactivity in the blood after intravenous injection of (3)H-A and (3)H-NA (3.7 MBq each) in 4 sheep (2 of each sex; total of 8 administrations) was determined. Blood samples were taken from the jugular vein. The highest radioactivity was observed in the first sample (5 min) following injection. Radioactivity showed a biphasic disappearance. An initial stage, in which radioactivity decreased rapidly (within 1 h) after the injection, was followed by a slow stage, lasting for up to 1 month, until background levels were reached. In vitro results indicated that NA and A were present not only in plasma (70%) but also in the erythrocytes (30%; mainly bound to haemoglobin). Sephadex G-25 gel filtration revealed that from the plasma fraction about 15% was strongly bound to proteins (mainly albumin). These results demonstrate that previous experiments in this field have overestimated the percentage of CAs bound to plasma proteins, because binding to haemoglobin was previously not known. In the future, efforts should be made to characterize the adduct products of CAs and establish an assay to determine them in vivo. If this could be achieved, it would yield a valuable tool for measuring the stress experienced for a longer period.

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A dopamine transporter in human erythrocytes: modulation by insulin

Cited by 16 publications

References 24 publications

Effect of incubation with crystalloid solutions or medications on packed red blood cells

Effect of incubation with crystalloid solutions or medications on packed red blood cells

Use of inotropes and vasopressor agents in critically ill patients

Binding and Clearance of Radioactive Adrenaline and Noradrenaline in Sheep Blood

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