A Dominant Locus, qBSC-1, Controls β Subunit Content of Seed Storage Protein in Soybean (Glycine max (L.) Merri.)

Abstract

Soybean seed storage protein is one of the most important plant vegetable protein, and β subunit is of great significance to enhance soybean protein quality and processing property. F2 segregated population and residual heterozygous lines (RHL) derived from the cross between Yangyandou (low level of β subunit) and Zhonghuang13 (Normal level of β subunit) were used for mapping of β subunit content. Our results showed that β subunit content was controlled by a single dominant locus, qBSC-1 (Beta subunit content), which was mapped to a region of 11.9cM on chromosome 20 in F2 population of 85 individuals. This region was narrowed down to 2.5 cM between BARCSOYSSR_20_0997 and BARCSOYSSR_20_0910 in RHL with a larger population size of 246 individuals. There were 48 predicted genes within qBSC-1 region based on the reference genome (Glyma 1.0, Williams 82), including the two copies of β subunit coding gene CG4. An InDel marker developed from a TT insertion in one copy of CG4 promoter region in Yangyandou cosegregrated with BARCSOYSSR_20_0975 within qBSC-1 region, suggesting that this InDel marker maybe a useful marker for MAS.

Quantitative Trait Loci (QTL) Mapping for Glycinin and β-Conglycinin Contents in Soybean (Glycine max L. Merr.)

“…Osman et al demonstrated that 11S plays a role in raw milk preservation and might be used as a food preservative. Wang et al mapped a single dominant locus associated with seed storage proteins in soybean using two different populations.…”

“…Some QTLs for storage protein in soybean have been identified in various environments and materials. Using 101 F 6 -derived recombinant inbred lines (RILs) developed from a cross between N87-984-16 and TN93-99, Panthee et al identified three QTLs for glycinin on linkage groups (LGs) D2, I, and L and two QTLs for β-conglycinin on LGs D2 and J. Wang et al mapped a single dominant locus, qBSC-1 , controlling the β subunit content of β-conglycinin in soybean by employing an F 2 segregated population (85 lines) and 246 residual heterozygous lines developed from a cross between Yangyandou and Zhonghuang 13. Overall, mapping QTLs for protein composition could provide information about genetic relationships, identify the genes involved in controlling the process, and improve both protein content and nutritional quality such as the content of sulfur-containing amino acids by increasing glycinin and decreasing β-conglycinin .…”

Identification of major QTLs for soybean seed size and seed weight traits using a RIL population in different environments

“…Besides, comparing the localization results of seed size traits in this study with the results of other related trait localization studies, eight QTLs ( qSL-4-1 , qSL-17-1 , qSL-20-1 , qSW-12-2 , qSW-15-2 , qSW-17-1 , qST-13-3 , qST-20-1 ) were found to overlap or interval overlap with QTLs related to yield traits from previous studies ( Mansur et al., 1993 ; Kabelka et al., 2004 ; Du et al., 2009 ; Palomeque et al., 2009 ; Liu et al., 2011 ; Wang X. et al., 2014 ). Fifteen QTLs ( qSL-6-2 , qSL-13-1 , qSL-17-1 , qSL-18-1 , qSL-20-1 , qSW-11-1 , qSW-12-1 , qSW-12-2 , qSW-20-2 , qSW-20-3 , qST-5-2 , qST-13-1 , qST-13-3 , qST20-1 , qST-20-2 ) overlapped QTLs associated with traits related to protein, oil and fatty acids ( Sebolt et al., 2000 ; Tajuddin et al., 2003 ; Bachlava et al., 2009 ; Qi et al., 2011 ; Eskandari et al., 2013 ; Mao et al., 2013 ; Wang J. et al., 2014 ; Fan et al., 2015 ; Warrington et al., 2015 ; Han et al., 2015 ; Leite et al., 2016 ; Fliege et al., 2022 ). Five QTLs ( qSW-15-1 , qSW-15-3 , qSW-18-2 , qST-4-1 and qSW-5-2 ) had overlapping intervals with QTLs related to seed-set of pod number and soybean maturity ( Yao et al., 2015 ; Ning et al., 2018 ).…”

Soybean Breeding on Seed Composition Trait

“…Three genes encoding 11S, AtCRU1, AtCRU2 and AtCRU3, have been verified in Arabidopsis thaliana [135]. Wang et al [136] mapped a QTL qBSC-1 (7S), which could regulate the SSP. Knockdown of 7S globulin subunits can change nitrogen content in transgenic soybean seeds [137].…”