A DNAzyme‐Based Colorimetric Paper Sensor for <i>Helicobacter pylori</i>

Ali, M. Monsur; Wolfe, Michael G; Tram, Kha; Gu, Jimmy; Filipe, Carlos D. M.; Li, Yingfu; Brennan, John D.

doi:10.1002/anie.201901873

Cited by 122 publications

(97 citation statements)

References 37 publications

(5 reference statements)

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“…The reduced catalytic activity of LP1FQ is not favourable for direct translation to rapid fluorescent detection. However, a variety of signal production mechanisms have been devised specifically for RNA‐cleaving DNAzymes [13, 20] . It is conceivable that some of these strategies can be used to convert the L. pneumophila ‐specific DNAzyme into a rapid and sensitive sensing system for on‐site detection of L. pneumophila in cooling tower water.…”

Section: Discussionmentioning

confidence: 99%

“…RFDs have been selected for a handful of common bacteria including Escherichia coli , [18] Clostridium difficile , [19] Helicobacter pylori , [20] and Klebsiella pneumoniae [21] . It has been well demonstrated that RFDs can be used as molecular recognition elements for the design of biosensors and bioassays for bacterial detection [18–25] . For example, RFD‐EC1, which was first reported in 2011 for E. coli , has been successfully incorporated into multiple biosensing platforms [25–33] .…”

Section: Introductionmentioning

confidence: 99%

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Selection and Characterization of an RNA‐Cleaving DNAzyme Activated by Legionella pneumophila

Rothenbroker

McConnell

et al. 2021

Angewandte Chemie

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Legionella pneumophila is a deadly bacterial pathogen that has caused numerous Legionnaires’ disease outbreaks, where cooling towers were the most common source of exposure. Bacterial culturing is used for L. pneumophila detection, but this method takes approximately 10 days to complete. In this work, an RNA‐cleaving fluorogenic DNAzyme, named LP1, was isolated. Extensive characterization revealed that LP1 is reactive with multiple infectious isolates of L. pneumophila but inactive with 25 other common bacterial species. LP1 is likely activated by a protein target, capable of generating a detectable signal in the presence of as few as 10 colony‐forming units of L. pneumophila, and able to maintain its activity in cooling tower water from diverse sources. Given that similar DNAzymes have been incorporated into many sensitive assays for bacterial detection, LP1 holds the potential for the development of biosensors for monitoring the contamination of L. pneumophila in exposure sources.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Selection and Characterization of an RNA‐Cleaving DNAzyme Activated by Legionella pneumophila

Rothenbroker

McConnell

et al. 2021

Angewandte Chemie

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“…Using the RNA‐cleaving DNAzyme, Brennan and co‐workers designed a colorimetric paper device for specific and sensitive detection of Helicobacter pylori . The DNAzyme‐enabled colorimetric paper device worked well as point‐of‐care diagnostics for monitoring pathogens in complex real samples and maintained full functions even after 130 day storage at ambient temperature (Figure B) . In addition, as the secondary or tertiary structures of DNAzyme had well been demonstrated, many detection strategies were designed based on the structural modifications.…”

Section: Dna Nanomaterialsmentioning

confidence: 99%

“…B) Working principle of the DNAzyme solution‐based test and the selectivity test using stool samples spiked with various bacterial species. Adapted with permission . Copyright 2019, Wiley‐VCH Verlag GmbH &Co. KGaA, Weinheim.…”

Section: Dna Nanomaterialsmentioning

confidence: 99%

Micro/Nano Technology for Next‐Generation Diagnostics

Gao

Tang

et al. 2019

Small Methods

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There has been a constant and urgent need to upgrade the current diagnostic technologies to the next generation by utilizing innovative advanced technologies in order to meet new challenges. Micro/nano technology, a series of methods for design and manipulation materials at the micrometer and nanometer scales, which perfectly covers the key building blocks of life including cells, organelles, proteins, nucleic acids, and other components, has unique advantages over current diagnostic methods. For the applications of micro–nano technology in next‐generation diagnostics, three categories, including microchip‐based devices, DNA nanomachines, and DNA nanomaterials, are extensively reviewed. Specifically, for the microchip‐based devices, terahertz technology and surface acoustic wave technology show their ultrasensitivity of label‐free, amplification‐free diagnosis. For the DNA nanomachines, DNA tweezers, DNA robots, and DNA walkers exhibit a great potential for diagnosis with the arbitrary structures and controllable motion behaviors. For the DNA nanomaterials, DNA aptamers, DNAzymes, and hybrid DNA nanomaterials provide sensitive biorecognition elements and versatile signal transductions, which are demonstrated as promising material candidates for the next‐generation diagnostics. Through this review, it is envisioned that micro/nano technologies will play an important role in the next‐generation diagnostics.

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“…At present, numerous intelligent detecting equipment have been supplied with plentiful sensors, extending their practical applications into diverse domains including industrial production, marine exploration, environmental protection, medical diagnosis, bioengineering, cosmic exploration, smart home. [1][2][3] With the growing demands for applications in the information age, the expectation and idealized requirements about abundant sensing performance of detecting range, accuracy, and stability have been gradually amplified. Aimed at the monitoring necessities of gas, stress, and temperature in the presented work provides state-of-the-art advances in 3D printed strain sensors, which is organized in three following sections.…”

Section: Introductionmentioning

confidence: 99%

3D Printed Flexible Strain Sensors: From Printing to Devices and Signals

et al. 2021

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The revolutionary and pioneering advancements of flexible electronics provide the boundless potential to become one of the leading trends in the exploitation of wearable devices and electronic skin. Working as substantial intermediates for the collection of external mechanical signals, flexible strain sensors that get intensive attention are regarded as indispensable components in flexible integrated electronic systems. Compared with conventional preparation methods including complicated lithography and transfer printing, 3D printing technology is utilized to manufacture various flexible strain sensors owing to the low processing cost, superior fabrication accuracy, and satisfactory production efficiency. Herein, up-to-date flexible strain sensors fabricated via 3D printing are highlighted, focusing on different printing methods based on photocuring and materials extrusion, including Digital Light Processing (DLP), fused deposition modeling (FDM), and direct ink writing (DIW). Sensing mechanisms of 3D printed strain sensors are also discussed. Furthermore, the existing bottlenecks and future prospects are provided for further progressing research.

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A DNAzyme‐Based Colorimetric Paper Sensor for Helicobacter pylori

Cited by 122 publications

References 37 publications

Selection and Characterization of an RNA‐Cleaving DNAzyme Activated by Legionella pneumophila

Selection and Characterization of an RNA‐Cleaving DNAzyme Activated by Legionella pneumophila

Micro/Nano Technology for Next‐Generation Diagnostics

3D Printed Flexible Strain Sensors: From Printing to Devices and Signals

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