A DNA walker triggered isothermal amplification method based on freezing construction of AuNP probes and its application in ricin detection

Abstract

DNA molecular machines are widely used in the fields of biosensors and biological detection. Among them, DNA Walker has attracted more attention due to its simple design and controllability. Herein,...

Multiple Isothermal Amplification Coupled with CRISPR–Cas14a for the Naked-eye and Colorimetric Detection of Aflatoxin B1

“…This red shift in absorption spectra for AuNPs is dependent on nanoparticle size, aggregation, and the local dielectric environments of the attached biomaterials . Therefore, this absorbance method is suitable for directly identifying binding events between AuNPs and DNA strands. ,− UV–vis spectroscopy indicates that the maximum absorbance peak of the AuNPs gradually shifts from 520 to 522 nm following DNA attachment (Figure C). This 2 nm red shift can be attributed to structural changes resulting from the successful modification of DNA on the AuNP surface.…”

Ultrasensitive Detection Strategy of Norovirus Based on a Dual Enhancement Strategy: CRISPR-Responsive Self-Assembled SNA and Isothermal Amplification

“…Furthermore, at −20 °C, the self-assembly reaction was completed at 30 min, and prolonging did not promote an additional DNA attachment to AuNPs. 37 However, the freezing effect (−20 °C) is related not only to the performance of the refrigerator itself but also to the size and placement of the sample. Thus, a freezing duration of 60 min was selected.…”

EXPAR for biosensing: recent developments and applications

“…Another study explored a DNA walker based on pre-assembled AuNP probes to trigger the EXPAR. 103 Notably, the freezing techniques employed in the DNA walker construction endow the AuNP probes with exceptional structural stability. Following the “recognition–cleavage–relative motion” cycle of the DNA walker reaction, the system initiates the exponential amplification process.…”