A DNA method for qualitative identification of plant raw materials in feedstuff

Tavoletti, Stefano; Iommarini, Linda; Pasquini, Marina

doi:10.1007/s00217-009-1077-1

Cited by 9 publications

(2 citation statements)

References 22 publications

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“…Feed composition therefore becomes an index of the raw materials used in the production chains and can be used as further information for the consumers to valorise the value of the final product. For these reason a DNA method has been developed as further result of the project aimed at the identification of the presence of faba beans and/or field pea within feed samples [28]. This could be a simple and not expensive approach to certify the use of local raw materials as feedstuff together with the absence of soybean from the ration.…”

Section: Critical Evaluation Of Soybean Role In Animal Production Chamentioning

confidence: 99%

Critical Evaluation of Soybean Role in Animal Production Chains Based on the Valorization of Locally Produced Feedstuff

Tavoletti¹

2013

Soybean - Bio-Active Compounds

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Section: Critical Evaluation Of Soybean Role In Animal Production Chamentioning

confidence: 99%

Critical Evaluation of Soybean Role in Animal Production Chains Based on the Valorization of Locally Produced Feedstuff

Tavoletti¹

2013

Soybean - Bio-Active Compounds

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“…Efficient methodologies for species authentication in processed foods and feeds, following different molecular approaches have been developed in the last few years [ 8 ]. However, very few reports, relying on both end-point and Real-time PCR, describe the use of those techniques to detect plant species in feed samples [ 9 – 11 ]. Real-time PCR instrumentation requires considerably short hands-on time, and detection of amplified products is automated, simple and reproducible [ 12 ].…”

Section: Introductionmentioning

confidence: 99%

A TaqMan real-time PCR method based on alternative oxidase genes for detection of plant species in animal feed samples

Campos¹,

Valadas²,

Campos³

et al. 2018

PLoS ONE

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Traceability of processed food and feed products has been gaining importance due to the impact that those products can have on human/animal health and to the associated economic and legal concerns, often related to adulterations and frauds as it can be the case for meat and milk. Despite mandatory traceability requirements for the analysis of feed composition, few reliable and accurate methods are presently available to enforce the legislative frame and allow the authentication of animal feeds. In this study, nine sensitive and species-specific real-time PCR TaqMan MGB assays are described for plant species detection in animal feed samples. The method is based on selective real-time qPCR (RT-qPCR) amplification of target genes belonging to the alternative oxidase (AOX) gene family. The plant species selected for detection in feed samples were wheat, maize, barley, soybean, rice and sunflower as common components of feeds, and cotton, flax and peanut as possible undesirable contaminants. The obtained results were compared with end-point PCR methodology. The applicability of the AOX TaqMan assays was evaluated through the screening of commercial feed samples, and by the analysis of plant mixtures with known composition. The RT-qPCR methodology allowed the detection of the most abundant species in feeds but also the identification of contaminant species present in lower amounts, down to 1% w/w. AOX-based methodology provides a suitable molecular marker approach to ascertain plant species composition of animal feed samples, thus supporting feed control and enforcement of the feed sector and animal production.

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Current Awareness in Phytochemical Analysis

2010

Phytochemical Analysis

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In order to keep subscribers up‐to‐date with the latest developments in their field, John Wiley &Sons are providing a current awareness service in each issue of the journal. The bibliography contains newly published material in the field of phytochemical analysis. Each bibliography is divided into 16 sections: 1 Reviews; 2 General; 3 Nucleic Acids; 4 Amino Acids, Proteins &Enzymes; 5 Carbohydrates; 6 Lipids; 7 Secondary Products ‐ General; Pharmacognosy; 8 Growth Regulators; 9 Industrially‐Important Products ‐ General; Food; Alcoholic beverages; 10 Toxins/Allergens; 11 Pigments; 12 Vitamins; 13 Others. Within each section, articles are listed in alphabetical order with respect to author. If, in the preceding period, no publications are located relevant to any one of these headings, that section will be omitted.

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A DNA method for qualitative identification of plant raw materials in feedstuff

Cited by 9 publications

References 22 publications

Critical Evaluation of Soybean Role in Animal Production Chains Based on the Valorization of Locally Produced Feedstuff

Critical Evaluation of Soybean Role in Animal Production Chains Based on the Valorization of Locally Produced Feedstuff

A TaqMan real-time PCR method based on alternative oxidase genes for detection of plant species in animal feed samples

Current Awareness in Phytochemical Analysis

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