A DNA Fingerprinting Approach for Distinguishing Native and Non-native Milfoils

Thum, Ryan A.; Lennon, Jay T.; Connor, Jody N.; Smagula, Amy P.

doi:10.1080/07438140609353879

Cited by 12 publications

(8 citation statements)

References 14 publications

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“…1 We performed molecular identifications of aquarium samples by comparing our sequences to those from Moody and Les' (2010) recent molecular systematic treatment of Myriophyllum. For all but six samples, we based our molecular analyses on DNA sequences from the nuclear ribosomal DNA internal transcribed spacers 1 and 2 and the intervening 5.8S ribosomal subunit (hereafter ITS), which has been shown to accurately distinguish species of Myriophyllum Les 2002, 2010;Thum et al 2006Thum et al , 2011. Methods for amplification and sequencing of ITS follow Thum et al (2011).…”

Section: Methodsmentioning

confidence: 99%

“…Myriophyllum species are notoriously difficult to identify morphologically (e.g., Aiken 1981;Moody and Les 2010) and as a result, some potentially harmful, misidentified species may be sold in the aquarium trade. However, most Myriophyllum species can be distinguished using molecular data (Moody and Les 2010;Thum et al 2006Thum et al , 2011, which offer promise for the accurate identification of taxa sold in the live aquarium trade.…”

Section: Introductionmentioning

confidence: 99%

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Loopholes in the regulation of invasive species: genetic identifications identify mislabeling of prohibited aquarium plants

2011

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Numerous invasive aquatic species introductions can be traced to the aquarium trade. Many potentially harmful aquarium species may be difficult to identify based on morphology alone. As such, some prohibited or invasive species may be available for purchase if they are mislabeled as species without restrictions. Here we compare molecular identifications to internet vendors' identifications for accessions of a popular genus of aquarium plants that are difficult to distinguish morphologically (Myriophyllum; watermilfoils). Specifically, we identified the extensive mislabeling of M. heterophyllum-an invasive species in the northeastern and western US. Furthermore, genotypes of M. heterophyllum found in our aquarium survey have also been found in invasive populations, suggesting their potential introduction through escape from aquaria, water gardens, or nurseries. Two additional taxa were sold under incorrect names. Finally, our survey revealed that Myriophyllum taxa present in the aquarium trade generally have poorly known distributions and ecologies, and therefore their invasive potential is unknown. Our study confirms that molecular identification methods can provide a valuable tool to survey commercial pathways for potentially harmful species that are otherwise difficult to identify.

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Loopholes in the regulation of invasive species: genetic identifications identify mislabeling of prohibited aquarium plants

2011

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“…We randomly subsampled at least 20 plants for analysis from lakes with more samples. To distinguish Eurasian, hybrid, and northern watermilfoil, plants were identified to taxon using a genetic assay based on internal transcribed spacer (ITS) DNA sequence [9,33]. Genetic variation was quantified for sampled plants and specific clones were delineated using eight microsatellite markers developed by Wu et al [34] [33] were used to amplify each microsatellite locus.…”

Section: Geneticsmentioning

confidence: 99%

“…Hybrid species may thus have increased likelihood of survival and establishment success in novel habitats. Although most research on invasive hybrids has focused on terrestrial and wetland herbaceous plants [5,7], hybrids have been associated with invasiveness in woody plants [8], submersed aquatic plants [3,9] and animals [10]. Therefore, identifying hybrids and the factors associated with their occurrence and spread is important to understanding biological invasions and informing management of invasive species.…”

Section: Introductionmentioning

confidence: 99%

Factors Influencing the Distribution of Invasive Hybrid (Myriophyllum Spicatum x M. Sibiricum) Watermilfoil and Parental Taxa in Minnesota

2020

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Eurasian watermilfoil (Myriophyllum spicatum L.) hybridizes with the native northern watermilfoil (M. sibiricum Kom.), which raises new issues regarding management strategies to control infestations. To determine the distribution of hybrid (and coincidentally Eurasian and northern) watermilfoil in Minnesota, we sampled lakes across the state during 2017–2018 for watermilfoil. A total of 62 lakes were sampled, spanning a range of sizes and duration of invasion. Forty-three lakes contained Eurasian, 28 contained hybrid and 21 contained northern watermilfoil. Eurasian watermilfoil populations were widespread throughout the state. Hybrid populations were more commonly found in lakes in the seven county Twin Cities Metro and northern watermilfoil populations were more commonly found in lakes outside of the Metro area. We found no evidence that hybrid watermilfoil occurred in lakes environmentally different than those with Eurasian and northern watermilfoil, suggesting that hybrid watermilfoil is not associated with a unique niche. Hybrid watermilfoil presence was significantly associated with the Metro area, which may likely be due to spatial and temporal factors associated with hybrid formation and spread. Hybrid watermilfoil presence was also significantly associated with lakes that had more parking spaces and older infestations, but this relationship was not significant when the effect of region was considered. Hybrid watermilfoil populations were the result of both in situ hybridization and clonal spread and continued assessment is needed to determine if particularly invasive or herbicide-resistant genotypes develop.

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“…Concernant les Myriophylles présents aux Etats‐Unis la confusion phénotypique a déjà été prouvée avec M. hippuroides , M. farwellii , M. humile , M. laxum, M. pinnatum & M. verticillatum (Thum et al ., ; Thum & Lennon, 2010). L'approche moléculaire permettrait seule de répondre dans certains cas à une identification délicate (Ghahramanzadeh et al ., ) et peut participer à la reconstitution de l'histoire des invasions biologiques (Thum et al ., ).…”

Section: Identité Du Myriophylleunclassified

Myriophyllum heterophyllum Michaux [Haloragaceae] en Haute‐Vienne (Limousin, France), et situation de cette plante invasive en France et en Europe

Lebreton

2013

EPPO Bulletin

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Myriophyllum heterophyllum Michaux [Haloragaceae] en Haute-Vienne (Limousin, France), et situation de cette plante invasive en France et en Europe Le 10 juillet 2011, sur la commune de Saint-Sylvestre en Haute-Vienne, une plante inhabituelle qui emerge massivement dans un etang priv e est observ ee. Une d etermination collective avec le Conservatoire botanique national du Massif Central (CBNMC) antenne Limousin, fait conclure a Myriophyllum heterophyllum Michaux. L'article fait le point sur cette esp ece consid er ee comme exotique envahissante en Europe mais qui n'avait pas fait l'objet d'un signalement d edi e ni d'un bilan sur sa situation en France et en Europe en milieu naturel.

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A DNA Fingerprinting Approach for Distinguishing Native and Non-native Milfoils

Cited by 12 publications

References 14 publications

Loopholes in the regulation of invasive species: genetic identifications identify mislabeling of prohibited aquarium plants

Loopholes in the regulation of invasive species: genetic identifications identify mislabeling of prohibited aquarium plants

Factors Influencing the Distribution of Invasive Hybrid (Myriophyllum Spicatum x M. Sibiricum) Watermilfoil and Parental Taxa in Minnesota

Myriophyllum heterophyllum Michaux [Haloragaceae] en Haute‐Vienne (Limousin, France), et situation de cette plante invasive en France et en Europe

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