A DNA Duplex with Extremely Enhanced Thermal Stability Based on Controlled Immobilization on Gold Nanoparticles

Akamatsu, Kensuke; Kimura, Minami; Shibata, Yoko; Nakano, Shu‐ichi; Miyoshi, Daisuke; Nawafune, Hidemi; Sugimoto, Naoki

doi:10.1021/nl0524748

Cited by 46 publications

(32 citation statements)

References 31 publications

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“…42 Immobilization of DNA onto gold has also been reported to enhance its thermal stability. 48 Polyvalent and PEGylation approaches are also reported to stabilize DNA in conjunction with gold. 49,50 For example, PEG, possessing a pentaethylenehexamine at one end of each polymer chain (N6-PEG), acts as a surface-modifier in cooperation with the cations of salts in physiological conditions to reinforce the formation of the GNPs.…”

mentioning

confidence: 99%

Functionalized gold nanoparticles for the binding, stabilization, and delivery of therapeutic DNA, RNA, and other biological macromolecules

DeLong¹,

Wanekaya²,

Reynolds³

et al. 2010

NSA

180

102

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Nanotechnology has virtually exploded in the last few years with seemingly limitless opportunity across all segments of our society. If gene and RNA therapy are to ever realize their full potential, there is a great need for nanomaterials that can bind, stabilize, and deliver these macromolecular nucleic acids into human cells and tissues. Many researchers have turned to gold nanomaterials, as gold is thought to be relatively well tolerated in humans and provides an inert material upon which nucleic acids can attach. Here, we review the various strategies for associating macromolecular nucleic acids to the surface of gold nanoparticles (GNPs), the characterization chemistries involved, and the potential advantages of GNPs in terms of stabilization and delivery.

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mentioning

confidence: 99%

Functionalized gold nanoparticles for the binding, stabilization, and delivery of therapeutic DNA, RNA, and other biological macromolecules

DeLong¹,

Wanekaya²,

Reynolds³

et al. 2010

NSA

180

102

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“…The unique properties, such as distance dependent surface plasmon effects that can result in dramatic color changes with extinction coefficients that are several hundred times higher than the best organic dyes, make them excellent sensing platform [6,16], which has been reviewed elsewhere [5,17]. For sensing and imaging in living cells, the AuNPs have been shown to confer greater stability of DNAs against degradation [18,19] and allow delivery of DNA into cells [20]. The first such demonstration is intracellular ATP detection in HeLa cells using DNA aptamer-functionalized AuNPs (Figure 2a) [21].…”

Section: Functional Dna and Gold Nanoparticlesmentioning

confidence: 99%

Functional DNA nanomaterials for sensing and imaging in living cells

Torabi

2014

Current Opinion in Biotechnology

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Recent developments in integrating high selectivity of functional DNA, such as DNAzyme and aptamers, with efficient DNA delivery into cells by gold nanoparticles or superior near-infrared optical properties of upconversion nanoparticles are reviewed. Their applications in sensing and imaging small organic metabolites, toxins, metal ions, pH, DNA, RNA, proteins, and pathogens are summarized. The advantages and future directions of these functional DNA materials are discussed.

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“…20 In these methods, the increased stability of the hybridization (or the affinity between the complementary oligonucleotides) has been achieved by substantially increasing the local concentrations of the oligonucleotides via assembling the two complementary oligonucleotides to a target molecule. Mirkin, et al has also reported the improved binding affinity for DNA-DNA interaction in gold nanoparticles attached DNAs (DNA-AuNPs) system, including sharp melting transitions [21][22][23][24][25] and increased binding constants. [26][27][28] The equilibrium binding constants (K eq ) of DNA-AuNPs with its complementary DNA are several orders of magnitude higher than their individual component DNA strands.…”

Section: Introductionmentioning

confidence: 99%

Nanoprobe-Enhanced, Split Aptamer-Based Electrochemical Sandwich Assay for Ultrasensitive Detection of Small Molecules

et al. 2015

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It is quite challenging to improve the binding affinity of antismall molecule aptamers. We report that the binding affinity of anticocaine split aptamer pairs improved by up to 66-fold by gold nanoparticles (AuNP)-attached aptamers due to the substantially increased local concentration of aptamers and multiple and simultaneous ligand interactions. The significantly improved binding affinity enables the detection of small molecule targets with unprecedented sensitivity, as demonstrated in nanoprobe-enhanced split aptamer-based electrochemical sandwich assays (NE-SAESA). NE-SAESA replaces the traditional molecular reporter probe with AuNPs conjugated to multiple reporter probes. The increased binding affinity allowed us to use 1,000-fold lower reporter probe concentrations relative to those employed in SAESA. We show that the near-elimination of background in NE-SAESA effectively improves assay sensitivity by ∼1,000-100,000-fold for ATP and cocaine detection, relative to equivalent SAESA. With the ongoing development of new strategies for the selection of aptamers, we anticipate that our sensor platform should offer a generalizable approach for the high-sensitivity detection of diverse targets. More importantly, we believe that NE-SAESA represents a novel strategy to improve the binding affinity between a small molecule and its aptamer and potentially can be extended to other detection platforms.

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A DNA Duplex with Extremely Enhanced Thermal Stability Based on Controlled Immobilization on Gold Nanoparticles

Cited by 46 publications

References 31 publications

Functionalized gold nanoparticles for the binding, stabilization, and delivery of therapeutic DNA, RNA, and other biological macromolecules

Functionalized gold nanoparticles for the binding, stabilization, and delivery of therapeutic DNA, RNA, and other biological macromolecules

Functional DNA nanomaterials for sensing and imaging in living cells

Nanoprobe-Enhanced, Split Aptamer-Based Electrochemical Sandwich Assay for Ultrasensitive Detection of Small Molecules

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