A DMP-triggered in vivo maternal haploid induction system in the dicotyledonous Arabidopsis

Zhong, Yu; Chen, Baojian; Li, Mengran; Wang, Dong; Jiao, Yanyan; Qi, Xiaozhe; Wang, Min; Liu, Zong-Kai; Chen, Chen; Wang, Yuwen; Chen, Ming; Li, Jinlong; Xiao, Zijian; Cheng, D.; Liu, Wenxin; Boutilier, Kim; Liu, Chenxu; Chen, Shaojiang

doi:10.1038/s41477-020-0658-7

Cited by 94 publications

(128 citation statements)

References 40 publications

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“…Our previous results identified ZmDMP orthologues in multiple species, but with an average amino acid sequence identity of 66%. Moreover 42% of dicots contain multiple DMP gene copies as a result of genome duplication and/or interspecific hybridization (Zhong et al, 2020). The relatively low sequence identity and the presence of multiple gene copies makes it difficult to accurately identify ZmDMP orthologues for the development of a DMP -HI system in dicot crops.…”

Section: Resultsmentioning

confidence: 99%

“…DMP genes with >50% amino acid identity were selected for expression analysis using public transcriptome databases. We identified: up to four DMP genes in rapeseed ( B. napus) (BnDMP1A/BnaA03g55920D; BnDMP1C/BnaC03g03890D ; BnDMP2A/BnaA04g09480D; BnDMP2C/BnaC04g31700D ) that are all highly expressed in anthers and flower buds; a single DMP gene in tomato ( Solanum lycopersicon ) ( SlDMP /Solyc05g007920) that is highly expressed in pollen and flower buds (Zhong et al, 2020); a single DMP gene in chili pepper ( Capsicum annuum ) ( CaDMP / Capana04g002148 ) that is highly expressed in closed flower bud and open flower; two cotton ( Gossypium hirsutum ) DMP genes ( GhDMP1 /LOC107911807; GhDMP2 /LOC107924398) that are expressed in the stamen; two soybean ( Glycine max ) DMP genes ( GmDMP1/GLYMA_18G097400; GmDMP2/GLYMA_18G098300 ) expressed in flower bud; and a single cucumber ( Cucumis sativus ) DMP gene ( CsDMP/Csa_1G267250 ) that is expressed in male flower bud (Supplemental Table 1). Expression data was not available for tobacco ( Nicotiana tabacum ), which contained three DMP genes ( NtDMP1 /LOC107762412; NtDMP2.…”

Section: Resultsmentioning

confidence: 99%

“…ZmPLA1/MTL/NLD genes have only been identified in monocots (Kelliher et al, 2017; Yao et al, 2018; Liu et al, 2019), while genes related to a second maize HI gene, Zea may DOMAIN OF UNKNOWN FUNCTION 679 membrane protein (ZmDMP ), have been identified in both monocots and dicots (Zhong et al, 2020). The utility of dmp mutants for HI in dicots was recently demonstrated in the model plant arabidopsis ( Arabidopsis thaliana ) (Zhong et al, 2020), but it is not known whether this approach can be translated to dicot crops. Here, we developed a method to identify candidate DMP genes for HI in crops and demonstrate DMP -mediated maternal HI in three major dicot crops with different ploidy levels and from two different plant families, tomato, rapeseed and tobacco.…”

Section: Introductionmentioning

confidence: 99%

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A genotype independent DMP-HI system in dicot crops

Zhong

Chen

Wang

et al. 2021

Preprint

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Doubled haploid (DH) technology is used to obtain homozygous lines in a single generation, which significantly accelerates the crop breeding trajectory. Traditionally, in vitro culture is used to generate DHs, but is limited by species and genotype recalcitrance. In vivo haploid induction (HI) through seed is been widely and efficiently used in maize and was recently extended to several monocot crops. However, a similar generic and efficient HI system is still lacking in dicot crops. Here we show that genotype-independent in vivo HI can be triggered by mutation of DMP genes in tomato, rapeseed and tobacco with HI rates of ~1.9%, 2.4% and 1.2%, respectively. The DMP-HI system offers a robust DH technology to facilitate variety improvement in these crops. The success of this approach and the conservation of DMP genes paves the way for a generic and efficient genotype-independent HI system in other dicot crops.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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A genotype independent DMP-HI system in dicot crops

Zhong

Chen

Wang

et al. 2021

Preprint

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“…A defective sperm may thus deliver both egg-activating factors and, occasionally, chromosomes, but fail to carry out proper karyogamy. This mechanism has not been explicitly proposed, to our knowledge, but may explain observations with the maize haploid induction system, where in addition to phospholipase, mutation of fusogenic proteins both enhance HI rate, and induce haploids in the wild-type phospholipase background (Zhong et al, 2020, 2019).…”

Section: Discussionmentioning

confidence: 96%

Rare instances of haploid inducer DNA in potato dihaploids and ploidy-dependent genome instability

Amundson

Ordoñez

Santayana

et al. 2020

Preprint

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In cultivated tetraploid potato, reduction to diploidy (dihaploidy) allows hybridization to diploid germplasm, introgression breeding, and may facilitate the production of inbreds. Pollination with haploid inducers yields maternal dihaploids, as well as triploid and tetraploid hybrids. It is not known if dihaploids result from parthenogenesis, entailing development of embryos from unfertilized eggs, or genome elimination, entailing missegregation and loss of paternal chromosomes. A sign of genome elimination is the occasional persistence of haploid inducer DNA in some of the dihaploids. We characterized the genomes of 1,001 putative dihaploids and 134 hybrids produced by pollinating tetraploid clones with three haploid inducers, IVP35, IVP101, and PL4. We detected inheritance of full or partial chromosomes from the haploid inducer parent in 0.87% of the overall dihaploid progeny, irrespective of the combination of parental genotypes. Chromosomal breaks commonly affected the paternal genome in the dihaploid and tetraploid progeny, but not in the triploid progeny. Residual haploid inducer DNA is consistent with genome elimination as the mechanism of haploid induction. Further, the fact that paternal chromosome breaks are specific to dihaploids and tetraploid progeny suggests that they may be specific to 2x sperms, and buttresses the hypothesis that 2x sperms facilitate genome elimination.

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“…The haploid inducer technology has been widely used in maize and other monocots (Kalinowska et al 2019) and, through wide crosses, in potato (Rokka 2009). Orthologues of the genes responsible for inducing haploids in monocots have been recently identified in dicots (Zhong et al 2020). There is ongoing research at CIAT targeting a sequence expected to induce haploids in cassava.…”

Section: Promising Arising Tools and Technologiesmentioning

confidence: 99%

Fifty years of a public cassava breeding program: evolution of breeding objectives, methods, and decision-making processes

Ceballos

Hershey²,

Iglesias

et al. 2021

Theor Appl Genet

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This paper reviews and analyzes key features from cassava breeding at the International Center for Tropical Agriculture (CIAT) over 50 years and draws lessons for public breeding efforts broadly. The breeding team, jointly with national program partners and the private processing sector, defined breeding objectives and guiding business plans. These have evolved through the decades and currently focus on four global product profiles. The recurrent selection method also evolved and included innovations such as estimation of phenotypic breeding values, increasing the number of locations in the first stage of agronomic evaluations, gradual reduction of the duration of breeding cycles (including rapid cycling for high-heritability traits), the development of protocols for the induction of flowering, and the introduction of genome-wide predictions. The impact of cassava breeding depends significantly on the type of target markets. When roots are used for large processing facilities for starch, animal feeding or ethanol production (such as in SE Asia), the adoption of improved varieties is nearly universal and productivity at the regional scale increases significantly. When markets and relevant infrastructure are weak or considerable proportion of the production goes for local artisanal processing and on-farm consumption, the impact has been lower. The potential of novel breeding tools needs to be properly assessed for the most effective allocation of resources. Finally, a brief summary of challenges and opportunities for the future of cassava breeding is presented. The paper describes multiple ways that public and private sector breeding programs can learn from each other to optimize success.

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A DMP-triggered in vivo maternal haploid induction system in the dicotyledonous Arabidopsis

Cited by 94 publications

References 40 publications

A genotype independent DMP-HI system in dicot crops

A genotype independent DMP-HI system in dicot crops

Rare instances of haploid inducer DNA in potato dihaploids and ploidy-dependent genome instability

Fifty years of a public cassava breeding program: evolution of breeding objectives, methods, and decision-making processes

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