A diverse proteome is present and enzymatically active in metabolite extracts
Rachel (Rae) J. House,
Molly T. Soper-Hopper,
Michael P. Vincent
et al.
Abstract:Metabolomics, a foundational tool in metabolism research, relies on the accurate transmittal of biochemical profiles underlying biological phenotypes. Over the years, workflows used in metabolomics have been assumed to remove enzymes to preserve metabolite levels during processing. Here, we uncover a diverse landscape of over 1,000 proteins, strongly enriched for metabolic enzymes, within metabolite extracts generated using common extraction workflows. Moreover, by combining in-extract stable isotope additions… Show more
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