A dive into the unknome

Rappsilber, Juri

doi:10.1016/j.tig.2023.10.011

Cited by 1 publication

(1 citation statement)

References 12 publications

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“…Even after decades of intensive research, the function of a large number of proteins remains unknown. This is even the case for well-studied model microorganisms such as Escherichia coli , Bacillus subtilis , and the bakers yeast Saccharomyces cerevisiae 1 as well as for the artificial minimal organism Mycoplasma mycoides JCVI-syn3A 2 . For the Gram-positive model bacterium B. subtilis , the function of about 25% of all proteins is completely unknown or only very vaguely understood 3 .…”

Section: Introductionmentioning

confidence: 99%

RfaA (YqhY), a novel adaptor protein, controls metabolite-sensitive protein degradation in Bacillus subtilis

Wicke,

Lentes,

Herrfurth

et al. 2024

Preprint

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The carboxylation of acetyl-CoA is the committed step of fatty acid synthesis catalyzed by the multisubunit acetyl-CoA carboxylase (ACCase). However, the mechanisms that control the activity of this enzyme are poorly understood. Here, we identify the so far unknown protein RfaA (YqhY) of the model bacterium Bacillus subtilis as a regulator of fatty acid acquisition that targets the AccC subunit of ACCase for degradation. RfaA interacts with both AccC and the ClpE unfoldase subunit of the ClpEP protease complex. While the former interaction is not sensitive to the physiological conditions, RfaA interacts with ClpE only in the absence of the global amino group donor glutamate. This results in the degradation of AccC in the absence of glutamate. The inactivation of the rfaA gene results in the accumulation of fatty acids in the cell and in the formation of lipid droplets which are toxic for the bacteria. The reduced fatty acid synthesis in the absence of glutamate as a result of RfaA- and ClpEP-dependent degradation of AccC thus prevents intoxication of the cells by fatty acids. Our findings suggest that the degradation of enzymes that catalyze the committed step of biosynthetic pathways might be an important mechanism to control cellular homeostasis.

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Section: Introductionmentioning

confidence: 99%