A distinct profile of myogenic regulatory factor detection within Pax7<sup>+</sup> cells at S phase supports a unique role of Myf5 during posthatch chicken myogenesis

Day, Kenneth; Paterson, Bruce M.; Yablonka–Reuveni, Zipora

doi:10.1002/dvdy.21903

Cited by 51 publications

(66 citation statements)

References 48 publications

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“…In porcine primary cells the expression of both Myf5 and MyoD decreased significantly during differentiation (Wilschut et al, 2010). This is in contrast to the observations in chicken primary cells, where Myf5 expression declined during differentiation while MyoD expression continued well into the differentiation step (Day et al, 2009). The latter is in line with our results.…”

Section: Discussioncontrasting

confidence: 75%

The combination of glycosaminoglycans and fibrous proteins improves cell proliferation and early differentiation of bovine primary skeletal muscle cells

et al. 2013

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Primary muscle cell model systems from farm animals are widely used to acquire knowledge about muscle development, muscle pathologies, overweight issues and tissue regeneration. The morphological properties of a bovine primary muscle cell model system, in addition to cell proliferation and differentiation features, were characterized using immunocytochemistry, western blotting and real-time PCR. We observed a reorganization of the Golgi complex in differentiated cells. The Golgi complex transformed to a highly fragmented network of small stacks of cisternae positioned throughout the myotubes as well as around the nucleus. Different extracellular matrix (ECM) components were used as surface coatings in order to improve cell culture conditions. Our experiments demonstrated improved proliferation and early differentiation for cells grown on surface coatings containing a mixture of both glycosaminoglycans (GAGs) and fibrous proteins. We suggest that GAGs and fibrous proteins mixed together into a composite biomaterial can mimic a natural ECM, and this could improve myogenesis for in vitroin vitro cell cultures.Abbreviations: used: ECMextracellular (The author would like to point out that there should be a space between the abbrevations and the subsequent explanation. ) matrix; GAGsglucosaminoglycans;MRFGAGsglucosaminoglycans, MRF, myogenic regulatory transcription factor; PGproteoglycan

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Section: Discussioncontrasting

confidence: 75%

The combination of glycosaminoglycans and fibrous proteins improves cell proliferation and early differentiation of bovine primary skeletal muscle cells

et al. 2013

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“…Satellite cell behavior is under stringent regulatory control in order to balance various actively maintained states, including quiescence, entry into proliferation and continuity of the cell cycle, and terminal differentiation Day et al 2009). Furthermore, apart from their ability to fortify myofibers and contribute to muscle regeneration, satellite cells have the capacity to replenish a reserve pool and self-renew, qualifying them as tissue-specific stem cells Sacco et al 2008).…”

Section: Functional Satellite Cells Are Required Throughout Lifementioning

confidence: 99%

“…In particular, expression of MyoD and myogenin has been used extensively in conjunction with Pax7 (Yablonka-Reuveni and Rivera 1994; Zammit, Carvajal et al 2004;Shefer et al 2006;Day et al 2009). Fig.…”

Section: Detection Of Satellite Cell Progeny By Temporal Expression Pmentioning

confidence: 99%

The Skeletal Muscle Satellite Cell

Yablonka–Reuveni

2011

J Histochem Cytochem.

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130

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SummaryThe skeletal muscle satellite cell was first described and named based on its anatomic location between the myofiber plasma and basement membranes. In 1961, two independent studies by Alexander Mauro and Bernard Katz provided the first electron microscopic descriptions of satellite cells in frog and rat muscles. These cells were soon detected in other vertebrates and acquired candidacy as the source of myogenic cells needed for myofiber growth and repair throughout life. Cultures of isolated myofibers and, subsequently, transplantation of single myofibers demonstrated that satellite cells were myogenic progenitors. More recently, satellite cells were redefined as myogenic stem cells given their ability to self-renew in addition to producing differentiated progeny. Identification of distinctively expressed molecular markers, in particular Pax7, has facilitated detection of satellite cells using light microscopy. Notwithstanding the remarkable progress made since the discovery of satellite cells, researchers have looked for alternative cells with myogenic capacity that can potentially be used for whole body cell-based therapy of skeletal muscle. Yet, new studies show that inducible ablation of satellite cells in adult muscle impairs myofiber regeneration. Thus, on the 50th anniversary since its discovery, the satellite cell's indispensable role in muscle repair has been reaffirmed. (J Histochem Cytochem 59:1041-1059, 2011

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“…For BrdU incorporation assays, cells were grown in DM for 4 days and further incubated for 3 hr in the presence of 5 mm BrdU. Cells were then fixed and treated as described (Day et al, 2009) before immunostaining with anti-BrdU antibody (DSHB, contributed by S.J. Kaufman).…”

Section: Experimental Procedures Antibodiesmentioning

confidence: 99%

Transitin is required for the differentiation of avian QM7 myoblasts into myotubes

Jalouli

Lapierre

Guérette

et al. 2010

Developmental Dynamics

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*Transitin is a nestin-like intermediate filament protein co-expressed with vimentin in the precursor cells of the myogenic and neurogenic lineages of the avian embryo. To understand its role in myogenesis, stable cell lines expressing transitin-targeted siRNAs were derived from the quail muscle cell line QM7. When cells were cultured in differentiation medium, we found that transitin knockdown prevented myoblast fusion and myotube formation. MyoD mRNA could be detected in transitin siRNA-transfected cells, but upregulation of myogenin and desmin expression was impaired compared to control cells. In addition, transitin siRNA cells maintain high levels of Pax7 expression suggesting that QM7 myoblasts into which transitin expression has been attenuated display a muscle progenitor cell phenotype (Pax7). These observations indicate that transitin plays an important role in the initiation of the myogenic program in avian muscle progenitor cells in acting downstream of MyoD and upstream of myogenin during the lineage progression. V C

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A distinct profile of myogenic regulatory factor detection within Pax7⁺ cells at S phase supports a unique role of Myf5 during posthatch chicken myogenesis

Cited by 51 publications

References 48 publications

The combination of glycosaminoglycans and fibrous proteins improves cell proliferation and early differentiation of bovine primary skeletal muscle cells

The combination of glycosaminoglycans and fibrous proteins improves cell proliferation and early differentiation of bovine primary skeletal muscle cells

The Skeletal Muscle Satellite Cell

Transitin is required for the differentiation of avian QM7 myoblasts into myotubes

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A distinct profile of myogenic regulatory factor detection within Pax7+ cells at S phase supports a unique role of Myf5 during posthatch chicken myogenesis

Cited by 51 publications

References 48 publications

The combination of glycosaminoglycans and fibrous proteins improves cell proliferation and early differentiation of bovine primary skeletal muscle cells

The combination of glycosaminoglycans and fibrous proteins improves cell proliferation and early differentiation of bovine primary skeletal muscle cells

The Skeletal Muscle Satellite Cell

Transitin is required for the differentiation of avian QM7 myoblasts into myotubes

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Product

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A distinct profile of myogenic regulatory factor detection within Pax7⁺ cells at S phase supports a unique role of Myf5 during posthatch chicken myogenesis