A Distal to Proximal Gradient of Human Choroid Plexus Development, with Antagonistic Expression of Glut1 and AQP1 in Mature Cells vs. Calbindin and PCNA in Proliferative Cells

Castañeyra-Ruiz, Leandro; González-Marrero, Ibrahim; Hernández-Abad, Luis G.; Carmona-Calero, Emilia M.; Meyer, Gundela; Castañeyra-Perdomo, Agustín

doi:10.3389/fnana.2016.00087

Cited by 14 publications

(6 citation statements)

References 22 publications

(40 reference statements)

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“…The choroid plexus is active in secretion of the cerebrospinal fluid (CSF) and in barrier functions early in corticogenesis (Castañeyra‐Ruiz et al. ). The secretion of signalling molecules from the ChP into the CSF can regulate the behaviour of neural stem cells, which are in direct contact with the CSF.…”

Section: Discussionmentioning

confidence: 99%

Cajal‐Retzius neurons are required for the development of the human hippocampal fissure

et al. 2019

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Cajal-Retzius neurons (CRN) are the main source of Reelin in the marginal zone of the developing neocortex and hippocampus (HC). They also express the transcription factor p73 and are complemented by laterappearing GABAergic Reelin + interneurons. The human dorsal HC forms at gestational week 10 (GW10), when it develops a rudimentary Ammonic plate and incipient dentate migration, although the dorsal hippocampal fissure (HF) remains shallow and contains few CRN. The dorsal HC transforms into the indusium griseum (IG), concurrently with the rostro-caudal appearance of the corpus callosum, by GW14-17. Dorsal and ventral HC merge at the site of the former caudal hem, which is located at the level of the future atrium of the lateral ventricle and closely connected with the choroid plexus. The ventral HC forms at GW11 in the temporal lobe. The ventral HF is wide open at GW14-16 and densely populated by large numbers of CRNs. These are in intimate contact with the meninges and meningeal blood vessels, suggesting signalling through diverse pathways. At GW17, the fissure deepens and begins to fuse, although it is still marked by p73/Reelin + CRNs. The p73KO mouse illustrates the importance of p73 in CRN for HF formation. In the mutant, Tbr1/Reelin + CRNs are born in the hem but do not leave it and subsequently disappear, so that the mutant cortex and HC lack CRN from the onset of corticogenesis. The HF is absent, which leads to profound architectonic alterations of the HC. To determine which p73 isoform is important for HF formation, isoform-specific TAp73-and DeltaNp73deficient embryonic and early postnatal mice were examined. In both mutants, the number of CRNs was reduced, but each of their phenotypes was much milder than in the global p73KO mutant missing both isoforms. In the TAp73KO mice, the HF of the dorsal HC failed to form, but was present in the ventral HC. In the DeltaNp73KO mice, the HC had a mild patterning defect along with a shorter HF. Complex interactions between both isoforms in CRNs may contribute to their crucial activity in the developing brain.Sections were deparaffinized, hydrated, and boiled in 10 mM citrate buffer (pH 6) for 20 min for antigen retrieval, rinsed in Trisbuffered saline (TBS, pH 7.6, 0.05 M), and incubated in the primary antibodies overnight in a humid chamber. After rinsing, they were incubated in the corresponding biotinylated secondary antibodies (rabbit anti-mouse IgG or goat anti-rabbit IgG; Dako, Glostrup, Denmark), diluted at 1 : 200 in TBS, followed by incubation with avidin-biotin complex (ABC, DAKO) in TBS. Bound peroxidase was

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Section: Discussionmentioning

confidence: 99%

Cajal‐Retzius neurons are required for the development of the human hippocampal fissure

et al. 2019

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“…In the Lmx1aCre:: β-catenin LOF telencephalon, the CP appears dysmorphic and disorganized (Figure 2). From E14.5, some regions of the CP lose their characteristic ribbon-like appearance and fail to express the transcription factor Otx2 , which is required for CP specification (Johansson et al 2013), or Aquaporin 1 ( Aqp1 ), a channel critical for CSF production (Kim and Jung 2012; Castañeyra-Ruiz et al 2016). Though TTR expression appears relatively unaffected, the expression of E-CADHERIN is perturbed, consistent with the abnormally folded appearance of the CP.…”

Section: Resultsmentioning

confidence: 99%

Constitutive activation of canonical Wnt signaling disrupts choroid plexus epithelial fate

Parichha

Suresh

Chatterjee

et al. 2020

Preprint

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The choroid plexus (CP) secretes cerebrospinal fluid and is critical for the development and function of the brain. In the telencephalon, the CP epithelium (CPe) arises from the Wnt- and Bmp- expressing cortical hem. We examined the role of canonical Wnt signaling in CPe development and report that the mouse and human embryonic CPe expresses molecules in this pathway. Either loss of function or constitutive activation of β-catenin, a key mediator of canonical Wnt signaling, causes a profound disruption of mouse CPe development. Loss of β-catenin results in a dysmorphic CPe, while constitutive activation of β-catenin causes a loss of CPe identity and a transformation of this tissue to a hippocampal-like identity. Aspects of this phenomenon are recapitulated in human embryonic stem cell (hESC)-derived organoids. Our results indicate that canonical Wnt signaling is required in a precisely regulated manner for normal CP development in the mammalian brain.

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“…Expressions of various AQPs in different tissues have been shown to controlled by hormones (1,(18)(19)(20). Herak-Kramberger et al (21) found that post-pubertal AQP1 expressions in males were higher than females, in their study of sex differences in AQP1 in rat kidneys.…”

Section: Discussionmentioning

confidence: 99%

Are There Any Sex-Depended Differences in Water Transporting Proteins of Choroid Plexus in Mice?

Sözen¹,

Aytaç²,

Demır³

et al. 2020

J Basic Clin Health Sci

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Background: It is known that sex hormones show modulatory effects on the central nervous system (CNS). Various CNS pathologies show sex-related differences in terms of rates, symptoms, and the course of the disease. Some of the functions of choroid plexus (CP) are under the control of sex hormones. The aim of this descriptive study was to evaluate sex effects on CP water channels.Methods: Expressions of Aquaporin (AQP) 1, 2 and 4 proteins in CP were compared between in male and female Balb/C mice brains; and also in different stages of the estrous cycle, with immunohistochemistry.Results: No difference is observed for AQP 1, 2 and 4 immunoreactivities in male and female brain, including different stages of the estrous cycle. Conclusion:In conclusion, to the best of our knowledge, AQP2 expression was shown in the CP for the first time, and no sex hormonedependent differences were found in terms of expressions of AQP1, 2 and 4.

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A Distal to Proximal Gradient of Human Choroid Plexus Development, with Antagonistic Expression of Glut1 and AQP1 in Mature Cells vs. Calbindin and PCNA in Proliferative Cells

Cited by 14 publications

References 22 publications

Cajal‐Retzius neurons are required for the development of the human hippocampal fissure

Cajal‐Retzius neurons are required for the development of the human hippocampal fissure

Constitutive activation of canonical Wnt signaling disrupts choroid plexus epithelial fate

Are There Any Sex-Depended Differences in Water Transporting Proteins of Choroid Plexus in Mice?

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