A Dissolution Test for Finasteride in Immediate-Release Capsules

Santos, Olimpia Maria Martins; Santos, Ana Laura; Pereira, Gislaine Ribeiro; Bonfílio, Rudy; Araújo, Magali Benjamim

doi:10.14227/dt200313p25

Cited by 5 publications

(2 citation statements)

References 24 publications

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“…For curves to be considered similar, F2 values had to be close to 100. Generally, F2 values greater than 50 (i.e., 50–100) ensured the equivalence of the two curves. , …”

Section: Experimental Sectionmentioning

confidence: 95%

“…This is a consequence of RAHB in both S 1 S2, Supporting Information). Table S2 shows 24 In most entries (22), strong acids such as perchloric, hydrochloric, hydrobromic, nitric, or sulfuric acids are crystallized together. This reflects that a proton is mainly transferred to the amide carbonyl oxygen in the presence of strong acids, as occurs in the salts with the same protonation pattern described in this study.…”

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confidence: 99%

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Protonation Pattern, Tautomerism, Conformerism, and Physicochemical Analysis in New Crystal Forms of the Antibiotic Doxycycline

Santos¹,

Silva²,

Martins

et al. 2014

Crystal Growth & Design

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Doxycycline (DOX) is a tetracycline class drug that is used worldwide as a broad-spectrum antibiotic. Although its clinical importance and use have been known since the 1960s, only four crystal forms have been reported until now. These are doxycycline hyclate (DOX.HYC), which is a hydrochloride salt hemiethanolate-hemihydrate; its isomorphous hydrobromide, hydrochloride salt dihydrate (DOX·HCl·2H2O); and doxycycline monohydrate (DOX·H2O). Here we report the preparation of two new multicomponent molecular crystal forms of doxycycline and their crystal structure determination along with their melting temperature, aqueous solubility, and time-dependent dissolution profile. These crystal forms are a hydronitrate salt hemihydrate (DOX·HNO3·0.5H2O) and an acetic acid solvate dihydrate (DOX·HAc·2H2O). The two new doxycycline crystal forms were compared with known forms, including DOX·HCl·2H2O, the structure of which was redetermined in this work. The structural variability of the protonation patterns, tautomerism of the keto–enolate moieties, and conformation of the amide groups was observed for these compounds. While intramolecular rings assembled through resonance-assisted hydrogen bond (RAHB) were observed in both fused keto–enol moieties of all structures, DOX·HCl·2H2O and DOX·HNO3·0.5H2O have another RAHB encompassing the protonated amide carbonyl oxygen and the enolate oxygen. These two crystal forms have a net positive charge on their drug molecule as DOX·HYC. They crystallize with the N,N -dimethylamine and amide carbonyl groups protonated and the neighboring hydroxyl group deprotonated. DOX, by contrast, crystallizes as a zwitterion in DOX·HAc·2H2O similarly to DOX·H2O. Their amide carbonyl oxygens are not protonated, which differs from the salt forms. DOX·HNO3·0.5H2O presents as two tautomers that are similar to those of DOX·HYC, namely, T1, in which the enolate oxygen is next to the protonated amine group, and T2, with the carbonyl oxygen close to the protonated amine group. These tautomers also differ in their amide conformations due to a rotation of ca. 180° on the C–C bond axis of the amide group, which directs the protonated carbonyl oxygen toward the enolate oxygen. DOX·HCl·2H2O has only one T1-like tautomer and therefore only one amide conformation similar to that of T1. A T1-like keto–enolate tautomer is present in DOX·HAc·2H2O, which exhibits an amide conformation similar to that of T2. Thermal (DSC and TG) and infrared analysis and equilibrium solubility, dissolution profiles, and forced degradation studies were performed to both new and known DOX forms. The results were correlated with their structural features. DOX·HNO3·0.5H2O was the most soluble form. This new form was also more stable than the commercial DOX·HYC in the oxidation test and more stable than commercial DOX·H2O against acid and basic hydrolysis and in the photostability study. DOX·HNO3·0.5H2O and DOX·HYC (commercial form) were observed to have similar drug release behaviors from capsules (F2 > 50) and therefore they could be i...

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“…For curves to be considered similar, F2 values had to be close to 100. Generally, F2 values greater than 50 (i.e., 50–100) ensured the equivalence of the two curves. , …”

Section: Experimental Sectionmentioning

confidence: 95%

mentioning

confidence: 99%