A disposable, integrated loop-mediated isothermal amplification cassette with thermally actuated valves

Liu, Changchun; Mauk, Michael G.; Bau, Haim H.

doi:10.1007/s10404-011-0788-3

Cited by 45 publications

(26 citation statements)

References 50 publications

(57 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Also, two-step reverses transcription (RT)-LAMP assays were developed for identifying RNA of the pathogen as the viability marker [9]. Recently, microfluidic LAMP assay was introduced as a rapid and portable identification test [10]; however, the microfluidic LAMP would be useful in the point-of-care for diagnostic purposes [11].…”

Section: Introductionmentioning

confidence: 99%

Microfluidic method for rapid turbidimetric detection of the DNA of Mycobacterium tuberculosis using loop-mediated isothermal amplification in capillary tubes

Rafati

Gill

2014

Microchim Acta

View full text Add to dashboard Cite

We describe a microfluidic method for rapid isothermal turbidimetric detection of the DNA of Mycobacterium tuberculosis. Loop-mediated isothermal amplification is accomplished in capillary tubes for amplifying DNA in less than 15 min, and sensitivity and specificity were compared to conventional loop-mediated isothermal amplification (LAMP). The method can detect as little as 1 pg mL −1 DNA in a sample. Results obtained with clinical specimens indicated 90 % sensitivity and 95 % specificity for microfluidic LAMP in comparison to culture methods. No interference occurred due to the presence of nonspecific DNAs. The findings demonstrate the power of the new microfluidic LAMP test for rapid molecular detection of microorganisms even when using bare eyes.

show abstract

Section: Introductionmentioning

confidence: 99%

Microfluidic method for rapid turbidimetric detection of the DNA of Mycobacterium tuberculosis using loop-mediated isothermal amplification in capillary tubes

Rafati

Gill

2014

Microchim Acta

View full text Add to dashboard Cite

show abstract

“…Nucleic acid-based molecular diagnostics have been simplified with the advent of isothermal amplification methods such as loop-mediated isothermal amplification (LAMP), reverse transcription loop-mediated isothermal amplification (RT-LAMP) [62,63], nucleic acid sequence based amplification (NASBA) [64,65], recombinase polymerase amplification (RPA) [66,67], and helicase dependent amplification (HDA) [68]. Isothermal amplification assays are very suitable for chip-based POC diagnostic applications due to their simple operation, high amplification efficiency, and easy detection.…”

Section: Isothermal Nucleic Acids Amplification Methodsmentioning

confidence: 99%

Miniaturized devices for point of care molecular detection of HIV

et al. 2017

Self Cite

View full text Add to dashboard Cite

The HIV pandemic affects 36.7 million people worldwide, predominantly in resource-poor settings. Nucleic acid-based molecular detection of HIV plays a significant role in antiretroviral treatment monitoring for HIV patients, as well as diagnosis of HIV infection in infants. Currently available molecular diagnostic methods are complex, time-consuming and relatively expensive, thus limiting their use in resource-poor settings. Recent advances in microfluidics technology have made possible low-cost integrated miniaturized devices for molecular detection and quantification of HIV at the point of care. We review recent technical advances in molecular testing of HIV using microfluidic technology, with a focus on assays based on isothermal nucleic acid amplification. Microfluidic components for sample preparation, isothermal amplification and result detection are discussed and compared. We also discuss the challenges and future directions for developing an integrated “sample-to-result” microfluidic platform for HIV molecular detection.

show abstract

“…The sensitivity referred to less than 24 CFU/mL for bacteria and 8.6 fg/µL for DNA [57]. Chuang et al demonstrated a simple, low-cost surface plasmon resonance sensing cartridge based on the LAMP method for the one-site detection of hepatitis B virus [58][59][60][61][62][63][64][65][66]. The HBV template could be detected by an SPR-LAMP system in 17 minutes even at the detection limit of 2 fg/mL.…”

Section: Strategies To Miniaturize Isothermal Amplification Reactionsmentioning

confidence: 99%

Isothermal Amplification and Quantification of Nucleic Acids and its Use in Microsystems

Tröger¹,

Niemann²,

Gärtig³

et al. 2015

J Nanomed Nanotechnol

View full text Add to dashboard Cite

Nucleic acid amplification technologies (NAATs) offer the most sensitive tests in the clinical laboratory. These techniques are used as a powerful tool for screening and diagnosis of infectious diseases. Isothermal methods, as an alternative to polymerase chain reaction (PCR), require no thermocycling machine and can mostly be performed with reduced time, high throughput, and accurate and reliable results.However, current molecular diagnostic approaches generally need manual analysis by qualified and experienced personal which is a highly complex, time-consuming and labor-intensive task. Thus, the demand for simpler, miniaturized systems and assays for pathogen detection is steadily increasing. Microfluidic platforms and lab-on-a-chip devices have many advantages such as small sample volume, portability and rapid detection time and enable point-of-care diagnosis.In this article, we review several isothermal amplification methods and their implementation in microsystems in relation to quantification of nucleic acids. miniaturized systems can be diverse. The majority of publications are focussed on clinical diagnostics including foodborne organisms for environmental monitoring [10][11][12]. In case of an infectious disease, cultivation and phenotypic characterization are still the standard methods of microbiologists which need days up to weeks to obtain a diagnostic result. Hence, scientists started to make use of nucleic acid amplification methods to accelerate the analysis. The most widespread and well known technique for this purpose is the polymerase chain reaction (PCR) [10,13], which exploits the activity of the DNA polymerase. The method is based on a thermal cycling process consisting of repeated cycles of heating and cooling steps allowing for DNA melting, sequence specific primer binding and enzymatic amplification of the target nucleic acid. The quantitative assessment of target copies is possible by using a real-time PCR approach, where a concentration dependent signal (e.g. fluorescence) increases over time [14]. The latter enables the user to easily assess the pathogen load of patient samples [15][16][17][18]. Since the first example of a PCR on a miniaturized system in 1994 [19,20], numerous devices were presented, which integrate not just the amplification, but also sample preparation and detection steps [9,[21][22][23][24][25][26][27][28]. Just a few of those microsystems have reached the market so far, but some have shown a fascinating potential to replace the classical laboratory-oriented state-of-the art [29][30][31][32][33]. setups has been shown to obtain a similar result [34]. A smaller heat capacity allows for rapid changes in temperature beneficial for the PCR time as well as a higher parallelism of multiple genetic samples [34].However, a major drawback of the integration of PCR to microsystems is the necessity of sophisticated instrumentation. The reaction requires a thermal cycling instrumentation, space and considerable expertise [35]. Therefore, isothermal reactions for the ta...

show abstract

A disposable, integrated loop-mediated isothermal amplification cassette with thermally actuated valves

Cited by 45 publications

References 50 publications

Microfluidic method for rapid turbidimetric detection of the DNA of Mycobacterium tuberculosis using loop-mediated isothermal amplification in capillary tubes

Microfluidic method for rapid turbidimetric detection of the DNA of Mycobacterium tuberculosis using loop-mediated isothermal amplification in capillary tubes

Miniaturized devices for point of care molecular detection of HIV

Isothermal Amplification and Quantification of Nucleic Acids and its Use in Microsystems

Contact Info

Product

Resources

About