A disposable electrochemical sensor based on protein G for High-Density Lipoprotein (HDL) detection

Chammem, Hanen; Hafaid, Imen; Bohli, Nadra; Garcı́a, Agustı́n Costa; Meilhac, Olivier; Abdelghani, Adnane; Mora, Laurence

doi:10.1016/j.talanta.2015.06.009

Cited by 11 publications

(5 citation statements)

References 26 publications

(30 reference statements)

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“…This directly allowed for combining higher amounts of the half-IgG antibody receptors [51,54], which had a crucial role in the sensitivity of the immunosensor. Therefore, these kinds of modifications based on covalent bonding from each side between Au and S can definitely guarantee a better and more stable SAM layer compared to the other immobilization systems based on the G or A proteins [31][32][33][34][35], which have relatively higher resistivity and, consequently, lower electrical signals towards the covalent immobilization of receptors through metal nanoparticles. Moreover, there are various methods for the determination of the collagen content, as summarised in Table 2.…”

Section: Discussionmentioning

confidence: 99%

“…For this purpose, it is fundamental to modify the surface of the working electrode to enhance the connection with the antibody receptor. Common methods are based on 'lock and key' approaches, such as G and A proteins, which enable immobilization of the receptor antibody on the surface of the transducer [31][32][33][34][35]. The application of G and A proteins is highly efficient for non-covalently binding antibodies, also providing to orientate them on-tail [36].…”

Section: Introductionmentioning

confidence: 99%

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Impedimetric and Plasmonic Sensing of Collagen I Using a Half-Antibody-Supported, Au-Modified, Self-Assembled Monolayer System

et al. 2021

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This research presents an electrochemical immunosensor for collagen I detection using a self-assembled monolayer (SAM) of gold nanoparticles (AuNPs) and covalently immobilized half-reduced monoclonal antibody as a receptor; this allowed for the validation of the collagen I concentration through two different independent methods: electrochemically by Electrochemical Impedance Spectroscopy (EIS), and optically by Surface Plasmon Resonance (SPR). The high unique advantage of the proposed sensor is based on the performance of the stable covalent immobilization of the AuNPs and enzymatically reduced half-IgG collagen I antibodies, which ensured their appropriate orientation onto the sensor’s surface, good stability, and sensitivity properties. The detection of collagen type I was performed in a concentration range from 1 to 5 pg/mL. Moreover, SPR was utilized to confirm the immobilization of the monoclonal half-antibodies and sensing of collagen I versus time. Furthermore, EIS experiments revealed a limit of detection (LOD) of 0.38 pg/mL. The selectivity of the performed immunosensor was confirmed by negligible responses for BSA. The performed approach of the immunosensor is a novel, innovative attempt that enables the detection of collagen I with very high sensitivity in the range of pg/mL, which is significantly lower than the commonly used enzyme-linked immunosorbent assay (ELISA).

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Impedimetric and Plasmonic Sensing of Collagen I Using a Half-Antibody-Supported, Au-Modified, Self-Assembled Monolayer System

et al. 2021

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“…Apo-A1 is a major constituent of high-density lipoproteins encoded on chromosome 11q23-q24 [17,18] and has a well-known anti-atherogenic function that protects against cardiovascular diseases [19][20][21][22][23]. The role of Apo-A1 in HCC is still unclear, but few recent reports have discussed the potential diagnostic role of Apo-A1 for early detection of HCV-related HCC [24,25].…”

Section: Discussionmentioning

confidence: 99%

Noninvasive prediction of hepatitis C-associated hepatocellular carcinoma using circulating apolipoproteins

et al. 2019

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Background and aims Hepatocellular carcinoma (HCC) is one of the leading causes of cancer-related mortality worldwide. We investigated the potential usefulness of circulating apolipoproteins (Apo-A1 and Apo-A4) in HCC screening and diagnosis. Patients and methods We included 60 adult patients with hepatitis C virus-related chronic liver disease including HCC, in addition to 20 healthy controls. Patients were stratified into three equal groups, with 20 patients each: chronic hepatitis C, posthepatitis C cirrhosis (liver cirrhosis), and HCC. All patients and controls underwent full clinical assessment, laboratory investigations, and evaluation of candidate apolipoproteins by enzyme-linked immunoassay. Results Significantly higher Apo-A1 and Apo-A4 levels were detected in patients with HCC than in those with liver cirrhosis (P<0.001). Receiver operator characteristic curve showed that for HCC diagnosis, a cutoff of 78.6 mg/dl for Apo-A1 yielded 90% sensitivity and 100% specificity and a cutoff of 16.5 mg/dl for Apo-A4 yielded 85% sensitivity and 80% specificity. Furthermore, within HCC group, Apo-A1 was significantly higher in patients with small HCC (>2 cm) than those with large tumors (P=0.01). Lower Apo-A1 level correlated significantly with pylethrombosis (P=0.007). Conclusion Apo-A1 and Apo-A4 are novel biomarkers for HCC screening and diagnosis, with a special discriminative ability for Apo-A1 for those with small tumors and those with pylethrombosis.

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“…Diabetes has been shown to accelerate the progression of atherosclerosis, which promotes the progression of coronary artery disease [10,11] and arteriosclerosis obliterans [12]. However, our understanding of the roles of insulin resistance and hyperglycemia in atherosclerosis is relatively incomplete [13,14]. Recently, some studies have discovered the factors involved in pyroptosis-induced atherosclerosis, such as shear stress, mircoRNAs (miRNAs) and nicotine [15][16][17].…”

Section: Introductionmentioning

confidence: 99%

CircRNA-DAPK1 promotes vascular cell pyroptosis in diabetes by regulating the circ-DAPK1/miR-4454/TXNIP axis.

Chen

Zheng

et al. 2021

Preprint

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Background Circular RNAs have been demonstrated to play an important role in the development of vascular diseases. However, little is known about the role of circ-021774, also named circ-DAPK1, in vascular cell pyroptosis. Methods Circ-DAPK1 was selected from circular RNA sequencing data of HUVECs treated with high glucose medium and normal medium. RT-qPCR was used to determine the expression of circ-DAPK1 in vivo and in vitro. Dual luciferase reporter assay, fluorescence in situ hybridization (FISH) and RNA immunoprecipitation (RIP) were performed to prove the interaction of circ-DAPK1, miRNA-4454 and thioredoxin-interactingprotein (TXNIP). Adeno-associated virus (AAV) was injected intravenously to establish mouse models. PI staining, western-blot and transmission electron microscopy (TEM) analyses were performed to identify the role of circ-DAPK1 in promoting pyroptosis. Results We found that circ-DAPK1 was highly expressed in high glucose medium cultured HUVECs and db/db mice. In vitro and in vivo experiments demonstrated that circ-DAPK1 knockdown decreased the number of PI+ cells, the expression of ASC, NLRP3, GSDMD-N, cleaved caspase-1, IL-18 and IL-1β. In a mechanistic study, the circ-DAPK1/miRNA-4454/TXNIP signaling axis was demonstrated to promote vascular cell pyroptosis in diabetes. Conclusions Circ-DAPK1 functions as a promoter of vascular cell pyroptosis in diabetes via the circ-DAPK1/miRNA-4454/TXNIP signaling axis.

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A disposable electrochemical sensor based on protein G for High-Density Lipoprotein (HDL) detection

Cited by 11 publications

References 26 publications

Impedimetric and Plasmonic Sensing of Collagen I Using a Half-Antibody-Supported, Au-Modified, Self-Assembled Monolayer System

Impedimetric and Plasmonic Sensing of Collagen I Using a Half-Antibody-Supported, Au-Modified, Self-Assembled Monolayer System

Noninvasive prediction of hepatitis C-associated hepatocellular carcinoma using circulating apolipoproteins

CircRNA-DAPK1 promotes vascular cell pyroptosis in diabetes by regulating the circ-DAPK1/miR-4454/TXNIP axis.

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