A disposable and cost efficient microfluidic device for the rapid chip-based electrical detection of DNA

Schuler, Thomas M.; Kretschmer, Robert; Jessing, Sven; Urban, Matthias; Fritzsche, Wolfgang; Möller, Robert; Popp, Jürgen

doi:10.1016/j.bios.2009.05.040

Cited by 31 publications

(23 citation statements)

References 30 publications

(28 reference statements)

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“…We further evaluated the performance of enzymatically generated silver nanoparticle (EGNP) deposition by immobilizing biotinylated single‐stranded DNA in the hydrogels. As seen in Figure , the silver deposition as an alternative readout increases with higher concentrations of biotinylated DNA in the gel.…”

Section: Resultsmentioning

confidence: 99%

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Easy Daylight Fabricated Hydrogel Array for Colorimetric DNA Analysis

Beyer

Pollok

Berg

et al. 2014

Macromolecular Bioscience

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The fabrication of 3D hydrogel microarrays for DNA analytics that allow simple visual signal readout for on-site applications is described. A convenient one-step polymerization of the hydrogel including in situ capture oligonucleotide immobilization is accomplished by using N,N'-dimethylacrylamide/polyethylene glycol (PEG1900 )-bisacrylamide monomers. The implementation of an acylphosphine-oxide photoinitiator even allows polymerization at daylight, whereas other approaches require exposure with light in the UV-range. This minimizes the risk of UV-caused DNA damages within the capture DNA-strand that could adversely affect the subsequent hybridization step. The porous network of these gel segments allows DNA as well as protein penetration. Thus, the successful in-gel DNA hybridization is monitored by the deposition of silver nanoparticles. These metal particles allow naked eye signal readout.

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Section: Resultsmentioning

confidence: 99%

“…Different approaches have been described in the literature to increase the porosity of gels. Carbon dioxide was used to create cavities within the gels . Also a PEG monomer solution or glycerol were used as none participating reagents in the polymerization reaction to function as spacers.…”

Section: Resultsmentioning

confidence: 99%

Easy Daylight Fabricated Hydrogel Array for Colorimetric DNA Analysis

Beyer

Pollok

Berg

et al. 2014

Macromolecular Bioscience

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“…[13] Compared to chip-based methods [14][15][16][17][18][19][20][21][22] the use of magnetic particles benefits from improved hybridization kinetics. By employing biotin-labeled primers for the amplification of target DNA with polymerase chain reaction (PCR), the PCR products can bind directly onto the magnetic beads.…”

Section: Magnetic Particlesmentioning

confidence: 99%

Discrimination of clostridium species using a magnetic bead based hybridization assay

Pahlow

Seise

Pollok

et al. 2014

Biophotonics: Photonic Solutions for Better Health Care IV

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Clostridium chauvoei is the causative agent of blackleg, which is an endogenous bacterial infection. Mainly cattle and other ruminants are affected. The symptoms of blackleg are very similar to those of malignant edema, an infection caused by Clostridium septicum. [1, 2] Therefore a reliable differentiation of Clostridium chauvoei from other Clostridium species is required. Traditional microbiological detection methods are time consuming and laborious. Additionally, the unique identification is hindered by the overgrowing tendency of swarming Clostridium septicum colonies when both species are present. [1, 3, 4] Thus, there is a crucial need to improve and simplify the specific detection of Clostridium chauvoei and Clostridium septicum.Here we present an easy and fast Clostridium species discrimination method combining magnetic beads and fluorescence spectroscopy. Functionalized magnetic particles exhibit plentiful advantages, like their simple manipulation in combination with a large binding capacity of biomolecules. A specific region of the pathogenic DNA is amplified and labelled with biotin by polymerase chain reaction (PCR). These PCR products were then immobilized on magnetic beads exploiting the strong biotin-streptavidin interaction. The specific detection of different Clostridium species is achieved by using fluorescence dye labeled probe DNA for the hybridization with the immobilized PCR products. Finally, the samples were investigated by fluorescence spectroscopy. [5]

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“…With the nanocolloid assembly, the fluorescently labelled target DNAs were trapped due to the hybridization and b biotin-labelled target DNA hybridizes to its specific probes; c streptavidin-horseradish peroxidase conjugate is bound to the biotin modification; d silver nanoparticles are deposited resulting from the enzymatic reaction. The readout could be performed by either electrical measurement or optical transmission measurement via the properties of the deposited silver nanoparticles (Schüler et al 2009b) concentrated at the cusp for detection. This method offered pico-molar sensitivity, rapid hybridization in less than 1 min at 100 pM concentration with no repeated washing, heating and other complicated hybridization buffers involved.…”

Section: Bead-based Dna Hybridization Assaysmentioning

confidence: 99%

Microfluidic DNA hybridization assays

Weng

Jiang

2011

Microfluid Nanofluid

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DNA hybridization is one of the most powerful techniques applied in diagnostic assays. Microfluidics provides a promising means to analyse small sample volumes, reduce reagent consumption and cost, shorten processing time as well as develop fast, sensitive and portable diagnostic tools. By coupling with the microfluidic technology, DNA hybridization assay can achieve high sensitivity, enhance hybridization kinetics and decrease the non-specific target-probe binding. The microfluidic-based DNA hybridization technology has a great potential for developing low-cost, rapid, automatic and point-of-care diagnostic devices. In this article, we provide an overview and summarize the recent advances on the merging of microfluidics to DNA hybridization assays. The advantages and disadvantages of various methods are discussed. Potential improvements required for these technologies are proposed as well.

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A disposable and cost efficient microfluidic device for the rapid chip-based electrical detection of DNA

Cited by 31 publications

References 30 publications

Easy Daylight Fabricated Hydrogel Array for Colorimetric DNA Analysis

Easy Daylight Fabricated Hydrogel Array for Colorimetric DNA Analysis

Discrimination of clostridium species using a magnetic bead based hybridization assay

Microfluidic DNA hybridization assays

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