A Disease Resistance Gene in Arabidopsis with Specificity for Two Different Pathogen Avirulence Genes

Bisgrove, Sherryl R.; Simonich, Michael T.; Smith, Nadine M.; Sattler, Airlie; Innes, Roger W.

doi:10.2307/3870003

Cited by 67 publications

(81 citation statements)

References 14 publications

(18 reference statements)

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“…Wild-type plants expressed gene-for-gene resistance against PstDC3000(avrB) due to the presence of the CC-NBS-LRR gene RPM1 (28,29). Although the response of the atgsnor1-1 line was indistinguishable from wild-type plants, RPM1-mediated protection was abolished in the atgsnor1-3 mutant line (Fig.…”

Section: Gsnor1 Controls Cellular Sno Levelsmentioning

confidence: 99%

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A central role forS-nitrosothiols in plant disease resistance

Feechan

Kwon

Yun

et al. 2005

Proc. Natl. Acad. Sci. U.S.A.

511

553

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Animal S-nitrosoglutathione reductase (GSNOR) governs the extent of cellular S-nitrosylation, a key redox-based posttranslational modification. Mutations in AtGSNOR1, an Arabidopsis thaliana GSNOR, modulate the extent of cellular S-nitrosothiol (SNO) formation in this model plant species. Loss of AtGSNOR1 function increased SNO levels, disabling plant defense responses conferred by distinct resistance (R) gene subclasses. Furthermore, in the absence of AtGSNOR1, both basal and nonhost disease resistance are also compromised. Conversely, increased AtGSNOR1 activity reduced SNO formation, enhancing protection against ordinarily virulent microbial pathogens. Here we demonstrate that AtGSNOR1 positively regulates the signaling network controlled by the plant immune system activator, salicylic acid. This contrasts with the function of this enzyme in mice during endotoxic shock, where GSNOR antagonizes inflammatory responses. Our data imply SNO formation and turnover regulate multiple modes of plant disease resistance.S-nitrosylation ͉ salicylic acid ͉ nitric oxide

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Section: Gsnor1 Controls Cellular Sno Levelsmentioning

confidence: 99%

“…In Col-0 plants, the product of the bacterial avirulence gene avrB is recognized by a single dominant R gene, RPM1 (28,29). Thus, PstDC3000 expressing avrB elicit a gene-for-gene resistance response in this Arabidopsis accession, leading to a reduction in bacterial growth.…”

Section: Gsnor1 Controls Cellular Sno Levelsmentioning

confidence: 99%

A central role forS-nitrosothiols in plant disease resistance

Feechan

Kwon

Yun

et al. 2005

Proc. Natl. Acad. Sci. U.S.A.

511

553

View full text Add to dashboard Cite

show abstract

“…Pseudomonas syringae pv. tomato (Pst) DC3000 expressing the avirulence gene, avrB (18), is recognized by the R protein RPM1 (18,19), triggering the expression of race-specific disease resistance in Arabidopsis accession, Col-0. Challenge of this plant line with avirulent PstDC3000(avrB) modified the S-nitrosylation status of a series of proteins including A. thaliana SA-binding protein 3 (AtSABP3; At3g01500) (20).…”

Section: Atsabp3 Is S-nitrosylated In Vivo and In Vitro-mentioning

confidence: 99%

S-Nitrosylation of AtSABP3 Antagonizes the Expression of Plant Immunity

Wang

Feechan

Yun

et al. 2009

Journal of Biological Chemistry

218

143

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“…avrB was originally cloned from P. syringae pv. glycinea and later was found to mediate the elicitation of an RPM1-dependent HR and resistance in A. thaliana Col (44). Unlike DC3000, hrpA and hrpS mutants fail to elicit an HR in Rio-Grande PtoR (Table 1).…”

Section: Production Of Multiple Exps By Dc3000 In a Hrp-inducingmentioning

confidence: 99%

Hrp pilus: An hrp -dependent bacterial surface appendage produced by Pseudomonas syringae pv. tomato DC3000

Roine

Wei

Yuan

et al. 1997

Proc. Natl. Acad. Sci. U.S.A.

323

279

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Hypersensitive response and pathogenicity (hrp) genes control the ability of major groups of plant pathogenic bacteria to elicit the hypersensitive response (HR) in resistant plants and to cause disease in susceptible plants. A number of Hrp proteins share significant similarities with components of the type III secretion apparatus and f lagellar assembly apparatus in animal pathogenic bacteria. Here we report that Pseudomonas syringae pv. tomato strain DC3000 (race 0) produces a filamentous surface appendage (Hrp pilus) of 6-8 nm in diameter in a solid minimal medium that induces hrp genes. Formation of the Hrp pilus is dependent on at least two hrp genes, hrpS and hrpH (recently renamed hrcC), which are involved in gene regulation and protein secretion, respectively. Our finding of the Hrp pilus, together with recent reports of Salmonella typhimurium surface appendages that are involved in bacterial invasion into the animal cell and of the Agrobacterium tumefaciens virB-dependent pilus that is involved in the transfer of T-DNA into plant cells, suggests that surface appendage formation is a common feature of animal and plant pathogenic bacteria in the infection of eukaryotic cells. Furthermore, we have identified HrpA as a major structural protein of the Hrp pilus. Finally, we show that a nonpolar hrpA mutant of P. syringae pv. tomato DC3000 is unable to form the Hrp pilus or to cause either an HR or disease in plants.Major groups of Gram-negative plant pathogenic bacteria belonging to genera Erwinia, Pseudomonas, Ralstonia, and Xanthomonas contain hypersensitive reaction and pathogenicity (hrp) genes. These genes control the ability of these bacteria to initiate interactions with plants, including elicitation of the hypersensitive reaction (HR), characterized by rapid localized death of plant cells at the pathogen infection site in resistant plants and causation of disease in susceptible plants (1, 2).hrp genes of Pseudomonas syringae are expressed in planta as a result of a regulatory cascade involving the gene products of hrpS and hrpR, positive transcriptional regulators, and of hrpL, an alternative sigma factor (3, 4). HrpL recognizes a consensus sequence motif (''harp box'') that has been identified in the upstream regions of many hrp and avr genes (4). The expression of hrp genes of many P. syringae pathovars can also be induced in vitro when bacteria are grown in defined minimal medium with low pH and containing certain sugars or sugar alcohols as carbon sources (5-7).The 25-kb hrp͞hrmA gene cluster of Pseudomonas syringae pv. syringae strain 61 is sufficient to enable nonpathogenic strains of Pseudomonas fluorescens and Escherichia coli to elicit the HR in nonhost plants (8). Sixteen of the 25 genes in this completely sequenced hrp͞hrmA gene cluster are either predicted or shown to be required for secretion of harpin Pss , a proteinaceous elicitor of the HR encoded by hrpZ (9, 10). Nine of these hrp genes, recently renamed hrc genes (11), are broadly conserved among P. syringae pathovars, Erwi...

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A Disease Resistance Gene in Arabidopsis with Specificity for Two Different Pathogen Avirulence Genes

Cited by 67 publications

References 14 publications

A central role forS-nitrosothiols in plant disease resistance

A central role forS-nitrosothiols in plant disease resistance

S-Nitrosylation of AtSABP3 Antagonizes the Expression of Plant Immunity

Hrp pilus: An hrp -dependent bacterial surface appendage produced by Pseudomonas syringae pv. tomato DC3000

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