A direct role for Wnt8 in ventrolateral mesoderm patterning

Baker, Kevin D.; Ramel, Marie‐Christine; Lekven, Arne C.

doi:10.1002/dvdy.22419

Cited by 26 publications

(30 citation statements)

References 50 publications

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“…Wnt8a (Lekven et al, 2001;Hikasa and Sokol, 2013;Baker et al, 2010) signaling plays additional roles during mesodermal development. During gastrulation, Wnt8a signaling regulates the expression of several mesodermal ventrolateral markers required for the maintenance and proliferation of non-axial mesoderm (Baker et al, 2010;Ramel et al, 2005). In addition, Wnt signaling originating in the lateral mesodermal precursors acts on anterior neuroectoderm to posteriorize the neural tissue (Erter et al, 2001).…”

Section: Introductionmentioning

confidence: 99%

PIAS-like protein Zimp7 is required for the restriction of the zebrafish organizer and mesoderm development

Moreno-Ayala

Schnabel

Salas‐Vidal

et al. 2015

Developmental Biology

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The Zmiz2 (Zimp7) protein and its homolog Zmiz1 (Zimp10) were initially identified in humans as androgen receptor co-activators. Sequence analysis revealed the presence of an SP-RING/Miz domain, which is highly conserved in members of the PIAS family and confers SUMO-conjugating activity. Zimp7 has been shown to interact with components of the Wnt/β-Catenin signaling pathway and with Brg1 and BAF57, components of the ATP-dependent mammalian SWI/SNF-like BAF chromatin-remodeling complexes. In this work, we analyze the role of zygotic Zimp7 in zebrafish development. We describe evidence indicating that Zimp7 is required for mesoderm development and dorsoventral patterning. Morpholino-mediated reduction of zygotic Zimp7 produced axial mesodermal defects that were preceded by up-regulation of organizer genes such as bozozok, goosecoid and floating head at the onset of gastrulation and by down-regulation of the ventral markers vox, vent and eve1 indicating loss of the ventrolateral mesoderm. Consistently, embryos overexpressing zimp7 RNA exhibited midline defects such as loss of forebrain and cyclopia accompanied by transcriptional changes directly opposite of those found in the morphants. In addition, the patterning of ventralized embryos produced by the overexpression of vox and vent was restored by a reduction of Zimp7 activity. Altogether, our findings indicate that Zimp7 is involved in transcriptional regulation of factors that are essential for patterning in the dorsoventral axis.

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Section: Introductionmentioning

confidence: 99%

PIAS-like protein Zimp7 is required for the restriction of the zebrafish organizer and mesoderm development

Moreno-Ayala

Schnabel

Salas‐Vidal

et al. 2015

Developmental Biology

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“…In zebrafish embryos, maternal Wnt/β-catenin signaling promotes the dorsal organizer formation before gastrulation (Bellipanni et al, 2006;Schneider et al, 1996). By contrast, zygotic Wnt/β-catenin signaling promotes ventrolateral mesodermal development to limit the organizer after the onset of gastrulation, and thereafter promotes posterior neural development (Baker et al, 2010;Erter et al, 2001;Lekven et al, 2001;Ramel et al, 2005).…”

Section: Introductionmentioning

confidence: 99%

Glutathione peroxidase 4 inhibits Wnt/β-catenin signaling and regulates dorsal organizer formation in zebrafish embryos

et al. 2017

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The Wnt/β-catenin signaling pathway plays pivotal roles in axis formation during embryogenesis and in adult tissue homeostasis. Glutathione peroxidase 4 (GPX4) is a selenoenzyme and participates in the reduction of peroxides. Its synthesis depends on the availability of the element selenium. However, the roles of GPX4 in vertebrate embryonic development and underlying mechanisms are largely unknown. Here, we show that maternal loss of zebrafish gpx4b promotes embryonic dorsal organizer formation, whereas overexpression of gpx4b inhibits the development of the dorsal organizer. Depletion of human GPX4 and zebrafish gpx4b (GPX4/ gpx4b) increases, while GPX4/gpx4b overexpression decreases, Wnt/β-catenin signaling in vivo and in vitro. Functional and epistatic studies showed that GPX4 functions at the Tcf/Lef level, independently of selenocysteine activation. Mechanistically, GPX4 interacts with Tcf/Lefs and inhibits Wnt activity by preventing the binding of Tcf/Lefs to the promoters of Wnt target genes, resulting in inhibitory action in the presence of Wnt/β-catenin signaling. Our findings unravel GPX4 as a suppressor of Wnt/β-catenin signals, suggesting a possible relationship between the Wnt/β-catenin pathway and selenium via the association of Tcf/Lef family proteins with GPX4.

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“…We evaluated whether Mcc can inhibit β-catenin-dependent activation of the −0.8 kb Xenopus Siamois luciferase reporter, and found that Mcc overexpression has no effect on reporter activation -neither synergy nor inhibition -upon co-injection of mRNA encoding either the canonical Wnt8 ligand, which normally suppresses dorsal organizer genes and patterns ventrolateral mesoderm, or its effector β-catenin (Baker et al, 2010) (supplementary material Fig. S2E).…”

Section: Introductionmentioning

confidence: 99%

The PDZ domain protein Mcc is a novel effector of non-canonical Wnt signaling during convergence and extension in zebrafish

et al. 2014

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During vertebrate gastrulation, a complex set of mass cellular rearrangements shapes the embryonic body plan and appropriately positions the organ primordia. In zebrafish and Xenopus, convergence and extension (CE) movements simultaneously narrow the body axis mediolaterally and elongate it from head to tail. This process is governed by polarized cell behaviors that are coordinated by components of the non-canonical, β-catenin-independent Wnt signaling pathway, including Wnt5b and the transmembrane planar cell polarity (PCP) protein Vangl2. However, the intracellular events downstream of Wnt/PCP signals are not fully understood. Here, we show that zebrafish mutated in colorectal cancer (mcc), which encodes an evolutionarily conserved PDZ domain-containing putative tumor suppressor, is required for Wnt5b/Vangl2 signaling during gastrulation. Knockdown of mcc results in CE phenotypes similar to loss of vangl2 and wnt5b, whereas overexpression of mcc robustly rescues the depletion of wnt5b, vangl2 and the Wnt5b tyrosine kinase receptor ror2. Biochemical experiments establish a direct physical interaction between Mcc and the Vangl2 cytoplasmic tail. Lastly, CE defects in mcc morphants are suppressed by downstream activation of RhoA and JNK. Taken together, our results identify Mcc as a novel intracellular effector of non-canonical Wnt5b/ Vangl2/Ror2 signaling during vertebrate gastrulation.

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A direct role for Wnt8 in ventrolateral mesoderm patterning

Cited by 26 publications

References 50 publications

PIAS-like protein Zimp7 is required for the restriction of the zebrafish organizer and mesoderm development

PIAS-like protein Zimp7 is required for the restriction of the zebrafish organizer and mesoderm development

Glutathione peroxidase 4 inhibits Wnt/β-catenin signaling and regulates dorsal organizer formation in zebrafish embryos

The PDZ domain protein Mcc is a novel effector of non-canonical Wnt signaling during convergence and extension in zebrafish

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