A direct protein-protein interaction is involved in the suppression of β-catenin transcription by retinoid X receptor α in colorectal cancer cells

Han, Anjia; Tong, Chang; Hu, Dong; Bi, Xiuli; Yang, Wancai

doi:10.4161/cbt.7.3.5455

Cited by 17 publications

(17 citation statements)

References 30 publications

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“…Overall, Achilles tendons lacking Sparc are significantly thinner compared to their age-matched wild type counterparts and seem less densely packed based on histological evaluation. Further, they are characterized by smaller fibrils, reminiscent of what has been described for the dermis of Sparc−/− mice17. Our findings also confirm previously published results that fibril diameters in healthy-aged wild type tendons follow a bimodal distribution with an increase in larger diameter fibrils43.…”

Section: Discussionsupporting

confidence: 91%

Pleiotropic roles of the matricellular protein Sparc in tendon maturation and ageing

Gehwolf

Wagner

Lehner

et al. 2016

Sci Rep

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Acute and chronic tendinopathies remain clinically challenging and tendons are predisposed to degeneration or injury with age. Despite the high prevalence of tendon disease in the elderly, our current understanding of the mechanisms underlying the age-dependent deterioration of tendon function remains very limited. Here, we show that Secreted protein acidic and rich in cysteine (Sparc) expression significantly decreases in healthy-aged mouse Achilles tendons. Loss of Sparc results in tendon collagen fibrillogenesis defects and Sparc−/− tendons are less able to withstand force in comparison with their respective wild type counterparts. On the cellular level, Sparc-null and healthy-aged tendon-derived cells exhibited a more contracted phenotype and an altered actin cytoskeleton. Additionally, an elevated expression of the adipogenic marker genes PPARγ and Cebpα with a concomitant increase in lipid deposits in aged and Sparc−/− tendons was observed. In summary, we propose that Sparc levels in tendons are critical for proper collagen fibril maturation and its age-related decrease, together with a change in ECM properties favors lipid accretion in tendons.

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Section: Discussionsupporting

confidence: 91%

Pleiotropic roles of the matricellular protein Sparc in tendon maturation and ageing

Gehwolf

Wagner

Lehner

et al. 2016

Sci Rep

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“…2, GAAATCAAAGTCAG ATGCTA. As described previously [16], 1.5 × 10 5 cells were plated per 6‐well plate in media containing 10% FBS to reach 50% confluency, and transient transfection of the siRNA was performed to result in a final siRNA concentration of 100 nM for SW620 cell line. The cells were harvested for Western blot analysis.…”

Section: Methodsmentioning

confidence: 99%

“…As described previously [16], whole‐cell lysates were incubated with specific AEG‐1 antibody at 4°C overnight using Catch and Release reversible Immunoprecipitation system (Upstate Biotechnology, Lake Placid, NY), followed by immunoblotting probed with anti‐β‐catenin.…”

Section: Methodsmentioning

confidence: 99%

Astrocyte elevated gene‐1 interacts with β‐catenin and increases migration and invasion of colorectal carcinoma

Zhang¹,

Qiu²,

Meng³

et al. 2012

Molecular Carcinogenesis

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To investigate the astrocyte elevated gene-1 (AEG-1) expression and its relationship with the clinicopathological features of colorectal carcinoma (CRC) and β-catenin signaling pathway. Real-time PCR, Western blot, immunohistochemistry, and immunofluorescence staining were performed to detect AEG-1 expression in CRC cell lines, 8 pairs of fresh CRC and adjacent nontumor tissues (ANT), 120 pairs of paraffin-embedded CRC specimens and ANT tissues, and 60 samples of lymph node metastatic CRC tissues. Scratch wound assay and transwell matrix penetration assay were performed to determine migration and invasion of SW480 cell lines with stable AEG-1 overexpression or SW620 cell lines with AEG-1 knockdown. AEG-1 expression was upregulated in CRC cell lines and tissues compared with ANT. Furthermore, AEG-1 expression level significantly correlated with UICC stage, and the N classification. AEG-1 overexpression significantly enhanced migration and invasion of SW480 cell lines. However, AEG-1 knockdown suppressed migration and invasion of SW620 cell lines. Meanwhile, there was a positive correlation between AEG-1 high expression and β-catenin nuclear expression in CRC. AEG-1 overexpression increased nuclear β-catenin accumulation in CRC cell lines. AEG-1 knockdown decreased nuclear β-catenin accumulation in CRC cell lines. Moreover, we firstly found that AEG-1 interacted with β-catenin in SW480 cell lines. Our results for the first time showed that AEG-1 interacted with β-catenin in CRC cells and AEG-1 expression was closely associated with progression of CRC. AEG-1 might be a potential therapeutic target in CRC.

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“…Both plasmids were purchased from Upstate Biotechnology (Lake Placid, NY, USA). As described previously, 20 LS174, SW480 and A549 cells were cotransfected with 2 µg of each plasmid. To correct for differences in transfection efficiency, cells were cotransfected with a Renilla reporter plasmid using LipofectAMINE 2000 (Invitrogen, Carlsbad, CA, USA) according to the manufacturer's instructions.…”

Section: Methodsmentioning

confidence: 99%

Anticancer activity of Panax notoginseng extract 20(S)‐25‐OCH3‐PPD: Targetting β‐catenin signalling

Bi¹,

Zhao²,

Fang³

et al. 2009

Clin Exp Pharma Physio

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1. The Wnt/beta-catenin pathway plays a critical role in carcinogenesis and so agents that target Wnt/beta-catenin may have potential in cancer prevention and therapy. The aim of the present study was to evaluate the anticancer activity of the novel natural product dammarane-type triterpene sapogenin (20(S)-25-OCH3-PPD; PPD25) isolated from the leaves of Panax notoginseng. 2. The anticancer activity of PPD25 was evaluated in three colon cancer cell lines and in one lung cancer cell line. The effects of PPD25 to inhibit proliferation and to induce apoptosis were evaluated. In addition, the potential mechanisms underlying the effects of PPD25 were investigated. 3. It was found that the addition of 5 or 25 micromol/L PPD25 to the culture medium significantly inhibited cell proliferation and induced apoptosis in all four cancer cell lines. Mechanistic studies revealed that PPD25 significantly reduced the expression of beta-catenin, a key mediator in the Wnt pathway, as well as transcriptional targets of beta-catenin, namely c-myc, cyclin D1, cdk4 and T cell factor (TCF)-4. In addition, beta-catenin/TCF transcriptional activity was significantly suppressed by PPD25. 4. The data demonstrate that the PPD25 exerts its anticancer effect by targetting beta-catenin signalling, suggesting that PPD25 may have potential as a chemotherapeutic and/or chemopreventive agent for colon and lung cancer.

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A direct protein-protein interaction is involved in the suppression of β-catenin transcription by retinoid X receptor α in colorectal cancer cells

Cited by 17 publications

References 30 publications

Pleiotropic roles of the matricellular protein Sparc in tendon maturation and ageing

Pleiotropic roles of the matricellular protein Sparc in tendon maturation and ageing

Astrocyte elevated gene‐1 interacts with β‐catenin and increases migration and invasion of colorectal carcinoma

Anticancer activity of Panax notoginseng extract 20(S)‐25‐OCH3‐PPD: Targetting β‐catenin signalling

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