A direct non-competitive idiometric enzyme immunoassay for serum oestradiol

Marès, Anne-Marie; Boever, J. De; Osher, J; Quiroga, Santiago; Barnard, Geoff; Kohen, Förtüne

doi:10.1016/0022-1759(94)00332-q

Cited by 26 publications

(14 citation statements)

References 9 publications

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“…Attempts to establish reagent excess assays for such molecules have anyway been made. These include the use of anti-idiotypic antibodies, anti-immunocomplex (anti-IC) antibodies, − and recombinant VH (variable heavy domain of antibody) and VL (variable light domain of antibody) to make an open sandwich immunoassay. , Among these attempts, the use of anti-IC binders closely resembles the two-site immunometric assay. However, development of anti-IC antibodies for small molecules by traditional animal immunization is a tedious and problematic procedure with low success rate .…”

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confidence: 99%

Broad-Spectrum Noncompetitive Immunocomplex Immunoassay for Cyanobacterial Peptide Hepatotoxins (Microcystins and Nodularins)

et al. 2016

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A broad-spectrum noncompetitive immunoassay allowing sensitive and simple detection of a group of similar compounds would be an ideal tool for screening low-molecular weight analytes (<2000 Da) having many variants. However, the development of an essential antibody pair capable of sandwich-type recognition of the analytes' small generic core structure is a demanding task due to limited space available for simultaneous binding of two different antibodies. We report here a generic noncompetitive assay for cyanobacterial microcystins (MCs) and nodularins (Nod), a group of structurally related small cyclic peptides (∼1000 Da) with more than 100 naturally occurring analogs. The assay is based on the unique combination of a generic anti-immunocomplex (anti-IC) single-chain fragment of antibody variable domain (scFv) and a monoclonal antibody capable of binding to an Adda-group (3-amino-9-methoxy-2,6,8-trimethyl-10-phenyldeca-4(E),6(E)-dienoic acid) present in all MCs/Nod. The anti-IC scFv was isolated from a large synthetic antibody library with phage display and used to develop a single-step sandwich-type noncompetitive immunocomplex assay. The sensitive time-resolved immunofluorometry-based assay is capable of detecting all the 11 tested commonly occurring hepatotoxins (MC-LR, -dmlR, -RR, -dmRR, -LA, -LY, -LF, -LW, -YR, -WR, and Nod-R) at concentration below 0.1 μg/L in a 1 h assay. Using MC-LR, the most studied toxic and widely distributed of the toxins, the calculated detection limits (based on blank + 3SD response) are ∼0.026 μg/L in 1 h and ∼0.1 μg/L in 10 min assay time. This is by far the fastest reported immunoassay for MCs and Nod with a detection limit far below the World Health Organization's guideline limit (1 μg/L of MC-LR equivalent in drinking water). The assay was validated with spiked tap and lake water as well as with environmental surface water samples. The developed assay provides a simple, rapid, and highly sensitive tool for the quantitative detection of MCs/Nod with the additional benefit of automation and high-throughput possibilities for large scale screening of drinking and environmental surface water samples. Furthermore, the study describes the first demonstration of the assay intended for the detection of an analyte group comprising similar low-molecular weight compounds exhibiting the benefits of a reagent excess type assay.

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confidence: 99%

Broad-Spectrum Noncompetitive Immunocomplex Immunoassay for Cyanobacterial Peptide Hepatotoxins (Microcystins and Nodularins)

et al. 2016

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“…Since the establishment of this method, Barnard's group has applied it to the detection of estradiol (Barnard & Kohen, 1990;Barnard et al, 1991;Mares et al, 1995) and progesterone (Barnard et al, 1995a) in serum and Estrone-3 Glucuronide (Barnard et al, 1995b) in urine， Kobayashi et al have applied it to the detection of UDCA 7-NAG (a bile acid metabolite) (Kobayashi et al, 2000;Kobayashi et al, 2003 a) and 11-deoxycortisol (Kobayashi et al 2003 b). More recently, Niwa et al (2009) established a noncompetitive-type ELISA for cortisol based on idiometric assay model.…”

Section: Anti-idiotype Antibody Based Noncompetitive Immunoassay (Idimentioning

confidence: 99%

Recent Progress in Noncompetitive Hapten Immunoassays: A Review

Fan¹

2012

Trends in Immunolabelled and Related Techniques

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“…Idiometric assay ( Figure 8a) and open sandwich immunoassay (Figure 8b) are novel noncompetitive immunoassay formats with small molecules detection potential. The former format has sucessfully applied in the detection of small molecules such as estradiol (Barnard & Kohen, 1990;Barnard et al, 1991;Mares et al, 1995), progesterone (Barnard et al, 1995), UDCA 7-NAG (a bile acid metabolite) (Kobayashi et al, 2000;, 11-deoxycortisol (Kobayashi et al 2003 b) and cortisol (Niwa et al, 2009). And the latter has successfully applied in the detection of gibberellin (Lee et al, 2008), benzaldehyde (Shirasu et al, 2009), zearalenone (Suzuki et al, 2007) and 11-Deoxycortisol (Ihara et al, 2009).…”

Section: Noncompetitive Immunoassay Of Pesticidesmentioning

confidence: 99%

Pesticides Immunoassay

Fan¹

2011

Pesticides - Strategies for Pesticides Analysis

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A direct non-competitive idiometric enzyme immunoassay for serum oestradiol

Cited by 26 publications

References 9 publications

Broad-Spectrum Noncompetitive Immunocomplex Immunoassay for Cyanobacterial Peptide Hepatotoxins (Microcystins and Nodularins)

Broad-Spectrum Noncompetitive Immunocomplex Immunoassay for Cyanobacterial Peptide Hepatotoxins (Microcystins and Nodularins)

Recent Progress in Noncompetitive Hapten Immunoassays: A Review

Pesticides Immunoassay

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