A Dilute-and-Shoot LC–MS/MS Method for Urinary Glyphosate and AMPA

Jaikwang, Pittaya; Junkuy, Anongphan; Sapbamrer, Ratana; Seesen, Mathuramat; Khacha-ananda, Supakit; Mueangkhiao, Patthawee; Wunnapuk, Klintean

doi:10.1007/s10337-019-03853-3

Cited by 16 publications

(12 citation statements)

References 17 publications

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“…Further studies are needed to explore the possibility of analyzing histamine with relatively recent extraction methods, such as “Quick, Easy, Cheap, Effective, Rugged and Safe” (QuEChERS), which is used for the determination of polar and nonpolar compounds [ 7 , 47 ] and “dilute and shoot”, which is useful to reduce the waste of solvents during analysis [ 48 , 49 ], in accordance with the principles of GAC.…”

Section: Resultsmentioning

confidence: 99%

Development of a Rapid and Eco-Friendly UHPLC Analytical Method for the Detection of Histamine in Fish Products

Cicero

Galluzzo

Cammilleri

et al. 2020

IJERPH

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We developed, validated, and confirmed with proficiency tests a fast ultra-high-performance liquid chromatography with diode array detector (UHPLC-DAD) method to determine histamine in fish and fishery products. The proposed method consists of two successive solid–liquid extractions: one with a dilute solution of perchloric acid (6%) and the second only with water. The instrumental analysis with UHPLC provides a very fast run time (only 6 min) with a retention time of approximately 4 min, a limit of quantification (LOQ) of 7.2 mg kg−1, a limit of detection (LOD) of 2.2 mg kg−1, a recovery around 100%, a relative standard deviation (RSD%) between 0.5 and 1.4, and an r2 of calibration curve equal to 0.9995. The method detected optimal values of the validation parameters and required a limited number of reagents in comparison to other methods reported in the literature. Furthermore, the method could detect histamine in a very short time compared with other methods. This method, in addition to being validated, precise, specific, and accurate, avoids wasting time, money, and resources, and limits the use of organic solvents.

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Section: Resultsmentioning

confidence: 99%

Development of a Rapid and Eco-Friendly UHPLC Analytical Method for the Detection of Histamine in Fish Products

Cicero

Galluzzo

Cammilleri

et al. 2020

IJERPH

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“…The analytical technique described by Jaikwang et al was used for glyphosate analysis [ 21 ] using liquid chromatography-tandem mass spectrometry (LC–MS/MS). The system used was the Agilent 1290 Infinity high-performance liquid chromatography system coupled with an Agilent 6460 triple quadrupole mass spectrometer and electrospray ionization (Agilent Technologies, Inc., Palo Alto, CA, USA).…”

Section: Methodsmentioning

confidence: 99%

“…The accuracy and precision were between 86–105%. The analytical limit of quantification (LOQ) of this method was 5 g/L, with a 2.5 g/L limit of detection (LOD) [ 21 ]. The samples with concentrations less than LOD were given the value LOD/square root 2 [ 22 ].…”

Section: Methodsmentioning

confidence: 99%

Effects of exposure to glyphosate on oxidative stress, inflammation, and lung function in maize farmers, Northern Thailand

et al. 2022

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Background Glyphosate is a herbicide which is commonly used in agricultural areas. However, previous studies on glyphosate exposure in farmers and their health are still scarce. Methods A longitudinal pre-post study was performed among maize farmers. Information from questionnaires, urine and blood samples, and lung function were collected a day before and a day after glyphosate application in the morning. The urine samples were analyzed using liquid chromatography-tandem mass spectrometry to detect glyphosate levels. Serum samples were analyzed to detect malondialdehyde (MDA), glutathione (GHS), and C-reactive protein (CRP) levels using thiobarbituric acid, dithiobisnitrobenzoic acid, and nephelometry, respectively. Lung function performances were measured using a spirometer. Results A total of 180 maize farmers met the study inclusion criteria. After glyphosate application, it was found that increased urinary glyphosate levels contributed to increased serum MDA (β = 0.024, 95% CI = 0.000, 0.0047) and decreased serum GHS (β = -0.022, 95% CI = -0.037, -0.007), FEV1 (β = -0.134, 95% CI = -0.168, -0.100), FEV1/FVC (β = -0.062, 95% CI = -0.082, -0.042) and PEF (β = -0.952, 95% CI = -1.169, -0.735). Conclusions Exposure to glyphosate during glyphosate application had significant effects on oxidative stress and lung function in maize farmers.

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“…malathion (boiling point 156 °C), dimethoate (boiling point 117 °C), both being challenging candidates for extraction from matrix, liquid chromatography and ESI-MS by virtue of being semi volatile. Also studied were glyphosate, a widely used water soluble, amphoteric, highly polar broad spectrum herbicide and its main metabolite aminomethylphosphonic acid (AMPA) a highly polar, amphoteric, difficult to retain on column compound (Jaikwang, 2020). The vary fact that we were able to grately suppress and control formation of metal ion adducts in such a hetrogenous group of compounds scattered all over the domain of organic chemistry itself speaks of the robustness, efficiency usefulness of the technique developed by us as discussed subsequently.…”

Section: Resultsmentioning

confidence: 99%

Controlling formation of metal ion adducts and enhancing sensitivity in Liquid Chromatography Mass Spectrometry

Marwah

Zimba

2020

JANS

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Formation of metal ion adducts in mass spectrometry, particularly in electrospray ionization liquid chromatography mass spectrometry (ESI-LC-MS), is a nightmare scenario for an analyst dealing with quantitative analysis. We have studied in detail the metal adduct formation and concluded that the use of fluorinated alkanoic acids along with formic acid and volatile ammonium salts was extremely useful in suppressing metal adduct formation in positive ion mode of ESI-LC-MS. The extremely high electronegativity of fluorine atom and unique electrostatic nature of C—F bond coupled with stereo-electronic interactions with neighboring bonds or lone pairs enables the polyfluorinated alkanoic acids in trapping highly electropositive ions (Na+, K+) thereby letting proton do its job efficiently. Addition of formic acid, trifluoroacetic acid, heptafluorobutyric acid and ammonium acetate was found to be extremely effective in controlling metal ion adducts and producing [M+H]+ ions almost exclusively resulting in significant increase in the sensitivity. This technique has been successfully used in our laboratory for the estimation of targeted and nontargeted analysis of pesticides, marine toxins, drugs and pharmaceuticals etc. in various matrices including environmental waters using liquid chromatography-time of flight mass spectrometer operated in all ion acquisition mode and triple quadruples (QQQ) in multiple reaction monitoring (MRM) mode.

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A Dilute-and-Shoot LC–MS/MS Method for Urinary Glyphosate and AMPA

Cited by 16 publications

References 17 publications

Development of a Rapid and Eco-Friendly UHPLC Analytical Method for the Detection of Histamine in Fish Products

Development of a Rapid and Eco-Friendly UHPLC Analytical Method for the Detection of Histamine in Fish Products

Effects of exposure to glyphosate on oxidative stress, inflammation, and lung function in maize farmers, Northern Thailand

Controlling formation of metal ion adducts and enhancing sensitivity in Liquid Chromatography Mass Spectrometry

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