A digital microfluidic diluter-based microalgal motion biosensor for marine pollution monitoring

Han, Shuang; Zhang, Qian; Zhang, Xingcai; Liu, Xianming; Lü, Ling; Wei, Junfeng; Li, Yuancheng; Wang, Yunhua; Zheng, Guang

doi:10.1016/j.bios.2019.111597

Cited by 62 publications

(33 citation statements)

References 16 publications

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“…Some species of microalgae are auto-fluorescent and the presence of these compounds affects their fluorescent emission spectrum [15]. These mechanisms are used to create a variety of label-free and amplification-free biosensors [16,17].…”

Section: Introductionmentioning

confidence: 99%

Single Chlamydomonas reinhardtii cell separation from bacterial cells and auto‐fluorescence tracking with a nanosieve device

et al. 2020

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A planar, transparent, and adaptable nanosieve device is developed for efficient microalgae/bacteria separation. In the proposed method, a sacrificial layer is applied with dual photolithography patterning to achieve a 1D channel with a very low aspect ratio (1:10 000). A microalgae/bacteria mixture is then introduced into the deformable PDMS nanochannel. The hydrodynamic deformation of the nanochannel is regulated to allow the bacteria cells to pass through while leaving the microalgae cells trapped in the device. At a flow rate of 4 μL/min, the supernatant collected from the device is indistinguishable from a control solution, indicating that nearly all the microalgae cells are trapped in the device. Additionally, this device is capable of single cell auto-fluorescence tracking. These microalgae cells demonstrate minimal photobleaching over 250 s laser exposure and could be used to monitor hazardous compounds in the sample with a continuous flow. This method will be valuable to purify microalgae samples containing contaminations and study single-cell heterogeneity.

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Section: Introductionmentioning

confidence: 99%

Single Chlamydomonas reinhardtii cell separation from bacterial cells and auto‐fluorescence tracking with a nanosieve device

et al. 2020

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“…Thus microalgal lipid screening could benefit from a more miniaturized, streamlined and integrated approach. The maneuverable electrowetting-on-dielectric (EWOD) behavior of micro-droplets, on variable-electrized surface, has impelled the development of digital microfluidic (DMF) systems for high-throughput micro-reactors (Gao et al, 2013), which have prop up a wide range of biological and chemical applications in sub-microliter/or nanoliter drop scales, such as cellbased screenings (Han et al, 2019), immunoassays (Rackus et al, 2015), polymerase chain reactions (Schell et al, 2012), and single crystal synthesis and crystallization (Josep et al, 2014). DMF achieves a droplet manipulation via surface-tension modulation induced by electrodes coated with dielectric and hydrophobic materials.…”

Section: Introductionmentioning

confidence: 99%

“…The first one is that the imperfect profiling of droplet hydrodynamics are inspired by the capacitance-sensing concept. In this work, the droplet-derived capacitance was determined in real time and was used to track position (Zhang et al, 2019). Thus, droplets can be automatically addressed in real time, without human observation, pre-calibration (like in voltage feedback, Shih et al, 2011 ), or image capturing/processing (like in visual feedback loop, Shin and Lee, 2010).…”

Section: Introductionmentioning

confidence: 99%

An integrated digital microfluidic bioreactor for fully automatic screening of microalgal growth and stress-induced lipid accumulation

Wang

Zhao

Liu

et al. 2020

Preprint

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Algae are promising feedstock of biofuel. The screening of competent species and proper fertilizer supply are of the most important tasks. To accelerate this rather slow and laborious step, we developed an integrated high-throughput digital microfluidic (DMF) system that uses discrete droplet to serve as micro-bioreactor, encapsulating microalgal cells. Based on the fundamental understanding of various droplet hydrodynamics induced by the existence of different sorts of ions and biological species, an incorporation of capacitance-based position estimator, electrode-saving-based compensation and deterministic splitting approach was performed to optimize the DMF bioreactor. Thus, it enables all processes (e.g. nutrient gradient generation, algae culturing and analyzing of growth and lipid accumulation) occurring automatically on-chip especially in a high-fidelity way. The ability of the system to compare different micro algal strains on chip was investigated. Also, the Chlorella sp. were stressed by various conditions and then growth and oil accumulation were analyzed and compared, which demonstrated its potential as a powerful tool to investigate microalgal lipid accumulation at significantly lower laborites and reduced time.

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“…Some species of microalgae are auto-fluorescent and the presence of these compounds affects their fluorescent emission spectrum (Marty et al, 1992). These mechanisms are used to create a variety of label-free and amplification-free biosensors (Gosset et al, 2018; Han et al, 2019).…”

Section: Introductionmentioning

confidence: 99%

Single Chlamydomonas reinhardi (C. reinhardtii) cell separation from bacterial cells and auto-fluorescence tracking with a nano-sieve device

Korensky

Chen

Bao

et al. 2020

Preprint

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16A planar, transparent, and adaptable nano-sieve device is developed for efficient 17 microalgae/bacteria separation. In our strategy, a sacrificial layer is applied in the dual 18 photolithography patterning to achieve a one-dimensional channel with a very low aspect 19 ratio (1:10,000). Microalgae/bacteria mixture is then introduced into the deformable 20 polydimethylsiloxane (PDMS) nano-channel. The hydrodynamic deformation of the nano-21 channel is regulated to allow the bacteria cells to pass through while leaving the microalgae 22 cells trapped in the device. At a flow rate of 4 μl/min, ~100% of the microalgae cells are 23 trapped in the device. Additionally, this device is capable of immobilizing single cells in a 24 transparent channel for auto-fluorescence tracking. These microalgae cells demonstrate 25 minimal photo-bleaching over 250 s laser exposure and can be used to monitor hazardous 26 *Manuscript Click here to view linked References 2 3 4 5 6 7 8 compounds in the sample with a continuous flow fashion. Our method will be valuable to 27 purify microalgae samples containing contaminations and study single cell heterogeneity. 28 29

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A digital microfluidic diluter-based microalgal motion biosensor for marine pollution monitoring

Cited by 62 publications

References 16 publications

Single Chlamydomonas reinhardtii cell separation from bacterial cells and auto‐fluorescence tracking with a nanosieve device

Single Chlamydomonas reinhardtii cell separation from bacterial cells and auto‐fluorescence tracking with a nanosieve device

An integrated digital microfluidic bioreactor for fully automatic screening of microalgal growth and stress-induced lipid accumulation

Single Chlamydomonas reinhardi (C. reinhardtii) cell separation from bacterial cells and auto-fluorescence tracking with a nano-sieve device

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