A digital image-based micro-flow-batch analyzer

Abstract

This paper introduces for the first time the combination of digital images taken with a webcam and a microflow-batch analyzer as a novel strategy for implementing quantitative chemical analysis. The digital imagebased micro-flow-batch analyzer (DIB-μFBA) was formed using urethane-acrylate resin, glass slides and ultraviolet lithography. The glass slides were used as sealant layers on both sides of the substrate urethane-acrylate and provide the necessary transparency to the microsystem to conduct studies using digital images. DIB-μFBA uses digital images obtained from a webcam with CCD sensor, based on the RGB (red-green-blue) color model. The analyzer was used for determining the tannins in green tea employing the well-known ferrous tartrate method. All standard solutions were prepared in-line, and all analytical processes were completed by simply changing the operational parameters in DIB-μFBA control software. The paired t test, at a 95% confidence level, showed no statistically significant differences between results obtained by DIB-μFBA and the spectrophotometric reference method. The proposed microsystem presented satisfactory physical and chemical properties while keeping the flexibility, versatility, robustness and multi-task characteristics of conventional flow-batch analysis. Therefore, it was possible to build a low-cost device with high sample throughput (about 190 h −1 ) and reduced reagent consumption (about 300 times less than the reference method), contributing to the basic principles of green chemistry and the advancement of micro-analytical procedures.

Estimate Mass Flow Rate of Dense Phased Powder Solids

“…If is the velocity value obtained from cross-correlation, and the value of load cell attached at the base of the known length channel -observing a steady flow is . The equation 5 can compute the average mass flow rate at a crosssection of the pipe (5) The equation is valid only for the steady state flow of the solid particles sliding along the bottom surface of the channel as a discrete dense phase material under gravity. If the particles pneumatically conveyed in air, they dispersed, and the chances of errors in load cell measurement are possible.…”

“…Although hazardous to health by radiation leakage and require proper shielding, the results of radiometric methods are quite promising in the case of high concentration of solid particles. [4], [5]. If an economical, trustworthy, simple, and robust solution is required, the electrostatic and capacitive sensors are the best choices.…”

Simple Cost-Effective Sequential Injection Lab at Valve with Remote Control Employing Everyday Communication Technology with a Webcam Camera Detector for the Determination of Iron and Phosphate as Model Analytes

“…Although, there are published works on the use of webcam cameras for colorimetric detection in flow analysis systems (Rodrigues and Lapa, 2010;Wongwilai et al, 2010;Andrade et al, 2013;Lima et al, 2013;Lima et al, 2014), thus far there has been no report of their use in a remotely controlled flow system.…”

Classification and quantitative optical analysis of liquid and solid samples using a mobile phone as illumination source and detector

“…Reagent mediated analyses have been extensively studied in which digital images describing color development of one-shot sensing spots were evaluated [4,6,8,10]. There has also been work done using digital images of solutions based on their spectral properties [11][12][13][14][15][16].…”