A differentiation antigen expressed selectively by a proportion of human blood cells: detection with a monoclonal antibody

Brooks, Doug A.; Bradley, John; Zola, Heddy

doi:10.3109/00313028209069036

Cited by 40 publications

(6 citation statements)

References 21 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The reactivity of B-CLL cells with FMC8 was rather variable and difficult to correlate with other parameters such as SIg or TI expression. FMC8 did, however, stain a large proportion of (Brooks et al, 1982). All lymphomas and leukemias tested reacted with BA1 and Y29.55.…”

Section: That Of the T I + B-nhl Ormentioning

confidence: 91%

Expression of the T1 (CD5, p67) surface antigen in B‐CLL and B‐NHL and its correlation with other B‐cell differentiation markers

Delia

Bonati

Giardini

et al. 1986

Hematological Oncology

View full text Add to dashboard Cite

The T1 surface antigen (CD5,p67) expression on blood lymphocytes (PBL) and lymphoid cells from lymph node biopsies (LN) from 31 patients with B-cell chronic lymphocytic leukemia (B-CLL) and 79 with B non-Hodgkin lymphoma (B-NHL), was detected in 25 B-CLL (80 per cent) and in 11 B-NHL (13 per cent) belonging to the following histologic subtypes: lymphocytic of CLL type (DLWD) one case, lymphoplasmacytoid (DLWD) four cases, centrocytic (DLPD) five cases, immunoblastic (DH) one case. All B-CLL and the T1 + B-NHL were also tested with monoclonal antibodies against the Common Acute Lymphoblastic Leukemia Antigen, B cells (FMC7, FMC8, BA1, Y29-55), T cells (OKT11a), HLA-DR and HLA-DQ monomorphic determinants. All the B-CLL and the T1+ B-NHL were CALLA-, BA1+, Y29.55+. FMC7+ cells were detected in large numbers six B-CLL (three T1+ and three T1-) and in four centrocytic lymphomas. FMC8 reacted with 70 per cent of leukemias (where it stained 30 per cent of neoplastic cells) and with 8/9 T+ B-NHL. HLA-DR and HLA-DQ molecules were detected in 100 per cent and 90 per cent of cases respectively. In vitro treatment of HLA-DQ- or T1- B-CLL with phorbol ester TPA led to the expression of these antigens as well as of the receptors for Interleukin 2 and MLR3 activation antigen. Surface membrane Ig (SIg) was detected in 79 per cent of cases, its density measured by FACS analysis varied, even markedly, from case to case. Among the B-CLL, cells with high SIg content were either T1+ or T1- and more likely FMC7+. The SIg- cases were seven B-CLL (five T1+ and two T1-) and two B-NHL, in which, however, cytoplasmic IgM was detected. This study reveals the existence of four major B-CLL subgroups: T1- SIg-, T1+ SIg+, T1+ SIg+, T1- SIg+. It also indicates that the T1 antigen may be transitionally present during B-cell differentiation and that its expression may precede that of SIg as supported by the in vitro studies. In addition, the finding that some B-NHL are T1+ suggests that they derive similarly to the B-CLL from a common progenitor.

show abstract

Section: That Of the T I + B-nhl Ormentioning

confidence: 91%

Expression of the T1 (CD5, p67) surface antigen in B‐CLL and B‐NHL and its correlation with other B‐cell differentiation markers

Delia

Bonati

Giardini

et al. 1986

Hematological Oncology

View full text Add to dashboard Cite

show abstract

“…The immunological phenotype was consistent with a myeloid origin as shown by the reactivity with My9 (20, 21) and MCS.2 (CD13). In addition these cells expressed 3C5, found in early myeloblasts and lymphoblasts (20), class I1 HLA determinants, and 2 non-lineage restricted antigens identified by FMC8 and RFB1, both found in early haemopoietic precursors (22,23). Tests with a large panel of B and T lymphoid-specific…”

Section: Discussionmentioning

confidence: 97%

Tseudo‐lymphoid‘ leukaemia with unusual features: Ultrastructural, immunological, cytogenetic and molecular studies

Matutes

Foroni

Amin

et al. 1987

European J of Haematology

View full text Add to dashboard Cite

An unusual case of ‘pseudo‐lymphoid’ leukaemia is described. The leukaemic cells resembled small, mature lymphocytes but lacked B‐ and T‐cell membrane markers as well as immunoglobulin (Ig) and T‐cell receptor gene rearrangements. They showed, instead, features of early myeloid cells since they expressed 2 myeloid antigens, CDW13 and My9, and displayed peroxidase activity demonstrable by electron microscopy (EM) on unfixed cells. Cytogenetic studies showed monosomy 5, t(4;17) (p12;p11), t(2;3)(p23;q14) and an abnormal chromosome 12. Abnormalities of chromosomes 4 and 5 have been previously associated with ‘pseudo‐lymphoid’ leukaemias. This case illustrates the value of sensitive methods for the characterization of blast cells and for the precise diagnosis of leukaemias with apparent ‘lymphoid’ morphology.

show abstract

“…The specificities of the McAb used were as follows: OKT, (CD3, T, p 19-29; mature T-cells) OKT4 (CD4, T, p55; helperhnducer T-cells), OKT6 (CDI, thy, p45; common thymocyte), OKTll (CD2, T, p50, E-receptor), OKT9 (transferrin receptor) OKTlO (immature thymocyte and replicating cells) (10). 3Al (CD7, T, p41; pan-T lymphocytes) (1 1); Leu-1 (CD5, T, p67; pan-T lymphocytes and a subset of neoplastic B-cells) (12); OKIa (class I1 HLA determinants); B1 (CD20, B, p35; B-lymphocytes) (14); FMC7 (a subpopulation of B-lymphocytes (15); FMC8 (CD9, nT-nB, p24), monocytes, platelets and immature cells) (16); J5 (CD10, anti-calla antigen nT-nB, gp 100, pre-B cells and granulocytes) (17).…”

Section: Methodsmentioning

confidence: 99%

Coexpression of T‐ and B‐markers in a lymphoproliferative disorder

Miguel

Foroni²,

González

et al. 1986

Scandinavian Journal of Haematology

View full text Add to dashboard Cite

An atypical case of lymhoproliferative disorder in which T‐ and B‐cell antigens were coexpressed in the neoplastic cells is described. The disease was characterised by hepatosplenomegaly, lymphadenopaty, a low WBC (5 × 109/1) and bone marrow infiltration. The predominant cell population (> 70%) comprised lymphoid cells with a range of nuclear irregularities and included some blast cells. 90% of the peripheral blood lymphocytes showed a mature T‐helper phenotype (E+, T11+, T3+, T4+, T8‐, T6‐, TdT‐) with coexpression of the specific B‐markers Bl and FMC7, in 90% and 50% of cells, respectively. HLA‐DR antigens were present in 55% of cells while surface and cytoplasmic immunoglobulins (Ig) were detected in < 10% of cells. Molecular investigations with appropiate probes showed evidence of T‐cell receptor gene rearrangement but no rearrangement for the genes of the Ig‐heavy and ‐ light chains. Cytogenetic studies revealed a translocation t(10;19) (p12; q13) in all the metaphases analyzed. This case demonstrates that the study of neoplastic cells with a battery of monoclonal antibodies may disclose the existence of a hitherto unrecognised lymphoid cell population with atypical expression of B‐ and T‐cell antigens. On the other hand, the presence of T‐cell receptor gene rearrangement indicates that this is a T‐cell disorder with the aberrant co‐expression of specific B‐cell markers.

show abstract

A differentiation antigen expressed selectively by a proportion of human blood cells: detection with a monoclonal antibody

Cited by 40 publications

References 21 publications

Expression of the T1 (CD5, p67) surface antigen in B‐CLL and B‐NHL and its correlation with other B‐cell differentiation markers

Expression of the T1 (CD5, p67) surface antigen in B‐CLL and B‐NHL and its correlation with other B‐cell differentiation markers

Tseudo‐lymphoid‘ leukaemia with unusual features: Ultrastructural, immunological, cytogenetic and molecular studies

Coexpression of T‐ and B‐markers in a lymphoproliferative disorder

Contact Info

Product

Resources

About