2012
DOI: 10.1371/journal.pone.0049292
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A Differential Genome-Wide Transcriptome Analysis: Impact of Cellular Copper on Complex Biological Processes like Aging and Development

Abstract: The regulation of cellular copper homeostasis is crucial in biology. Impairments lead to severe dysfunctions and are known to affect aging and development. Previously, a loss-of-function mutation in the gene encoding the copper-sensing and copper-regulated transcription factor GRISEA of the filamentous fungus Podospora anserina was reported to lead to cellular copper depletion and a pleiotropic phenotype with hypopigmentation of the mycelium and the ascospores, affected fertility and increased lifespan by appr… Show more

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Cited by 10 publications
(23 citation statements)
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“…556 genes of the grisea transcriptome were identified to be down-regulated with a factor of at least 3 and a p-value of ≤0.01 for differential expression [22]. The 1,202 genes, identified as being down-regulated during aging in this study, have 89 genes in common.…”
Section: Resultsmentioning
confidence: 66%
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“…556 genes of the grisea transcriptome were identified to be down-regulated with a factor of at least 3 and a p-value of ≤0.01 for differential expression [22]. The 1,202 genes, identified as being down-regulated during aging in this study, have 89 genes in common.…”
Section: Resultsmentioning
confidence: 66%
“…Previously we used SuperSAGE successfully to characterize the transcriptome of a specific long-lived mutant of P. anserina and compared it to the transcriptome of the wild type. Validation by qRT-PCR demonstrated the reliability of this method [22]. The data of the current longitudinal study, in which RNA was isolated from the same fungal individuals after a defined period of growth and subjected to a genome-wide SuperSAGE analyses, identified autophagy as a quality control pathway up-regulated late in the life of P. anserina at a time when transcripts, encoding components of other pathways (e.g., proteasome), are down-regulated.…”
Section: Introductionmentioning
confidence: 87%
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“…Since transcription of PaCtr3 , coding for a high affinity copper transporter, is dependent on the availability and activity of GRISEA, both in senescent wild-type strains as well as in the grisea mutant, copper-uptake is only possible via low affinity import. As a consequence, a switch from a copper-dependent standard to an iron-dependent alternative respiration, a strongly reduced generation of the mitochondrial superoxide anion and pronounced changes in gene expression are observed in the mutant 22 23 41 .…”
Section: Resultsmentioning
confidence: 99%
“…This function may link genetic, environmental and stochastic processes involved in aging and lifespan control and can help to explain unexpected and counter-intuitive experimental data. After having investigated genome-wide transcriptome profiles of P. anserina wild-type cultures of different age and of a long-lived mutant in which mitochondrial ROS generation is reduced compared to the wild type 22 23 , we now set out to analyze the impact of increased oxidative stress on global gene expression. Using paraquat (PQ) as a generator of the superoxide anion at the mitochondrial respiratory chain 24 25 , the site at which this ROS is generated also during normal aging, we experimentally induced strong cellular oxidative stress in wild-type cultures of different age and found that this treatment has profound effects on gene expression, growth and lifespan.…”
Section: Introductionmentioning
confidence: 99%