A differential evolution approach to estimate parameters in a temperature-dependent kinetic model for second generation ethanol production under high cell density with Spathaspora passalidarum

Biazi, Luiz Eduardo; Martinez-Jimenez, Fernan David; Bonan, C.I.D.G.; Soares, Lauren B.; Morais, Edvaldo Rodrigo de; Ienczak, Jaciane Lutz; Costa, Aline Carvalho da

doi:10.1016/j.bej.2020.107586

Cited by 15 publications

(37 citation statements)

References 52 publications

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“…Biazi et al (2020) and Bonan et al (2020) worked with hemicellulosic hydrolysates obtained from acid pretreatment and S. passalidarum . Biazi et al (2020) worked with a 1.4 L bioreactor and 900 mL of working volume using 30 g/L glucose, 75 g/L xylose, and 20 g/L of initial cell concentration at 30 °C, 48 h, 200 rpm, and a k L a of 8.0 h –1 . On the other hand, Bonan et al (2020) used 27.7 g/L of glucose and 66.3 g/L of xylose in a 3L bioreactor containing 1.4L of working volume, 17.2 g/L of initial cell concentration, at 30 °C, 48 h, 200 rpm, and a k L a of 4.9 h –1 .…”

Section: Resultsmentioning

confidence: 99%

“…Fermentation assays were performed using Spathaspora passalidarum NRRL Y-27907 kept on solid YPD agar medium at 4 °C. Inoculation was performed in two-steps: , first one stock was transferred to 250 mL Erlenmeyer flasks containing 100 mL of a medium containing (g/L): yeast extract (10.0); peptone (20.0); and glucose (20.0). The flasks were kept in a shaker for 24 h at 30 °C and 200 rpm.…”

Section: Methodsmentioning

confidence: 99%

“…Cell propagation was performed in 1000 mL Erlenmeyer flasks containing 250 mL of working media with the following composition (g/L): yeast extract (3.0); peptone (5.0); urea (5.0); glucose (30.0); and KH 2 PO 4 (2.0) with a pulse of glucose (30.0) at 24 h. The culture was kept in a shaker for 48 h at 30 °C and 200 rpm. After this time, cells were centrifuged at 4000 rpm for 20 min and resuspended in a minimum volume of sterile distilled water. , …”

Section: Methodsmentioning

confidence: 99%

“…After this time, cells were centrifuged at 4000 rpm for 20 min and resuspended in a minimum volume of sterile distilled water. 52,58 The cell suspension (8 g/L) was transferred to the fermentation medium containing (g/L): hydrolysate (50.0); yeast extract (3.0); urea (2.3); and MgSO 4 •7H 2 O (1.0). A control fermentation was carried out for comparison using the following fermentation medium (g/L): glucose (36.0) and xylose (14.0).…”

Section: ■ Materials and Methodsmentioning

confidence: 99%

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Effect of Protic Ionic Liquids in Sugar Cane Bagasse Pretreatment for Lignin Valorization and Ethanol Production

Rabelo

et al. 2021

ACS Sustainable Chem. Eng.

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Ionic liquids have been called designer solvents because it is possible to fine-tune their properties from a huge range of possible combinations of cations and anions. In this work, we evaluate the potential of protic ionic liquids (PILs) in the fractionation of sugar cane bagasse in a biorefinery context. The PILs were designed to selectively solubilize lignin and leave a polysaccharides solid fraction. The carbohydrate fraction was further hydrolyzed to obtain pentose and hexose sugars, which were fermented using a pentose-consuming yeast. The results have shown that, differently from pretreatments that solubilize hemicelluloses, pentoses could be fermented together with hexoses without a prior detoxification step with high yield and productivity. The lignin recovered was characterized by 2D HSQC NMR, 31 P NMR, and GPC, showing that the design of the PIL influences lignin characteristics, but pretreatment temperature and time do not. The lignins obtained are sulfur-free, and the richness of their functional groups makes them attractive for conversion into a diversity of value-added products, which can make a biorefinery based on the proposed process viable.

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Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: ■ Materials and Methodsmentioning

confidence: 99%

See 2 more Smart Citations

Effect of Protic Ionic Liquids in Sugar Cane Bagasse Pretreatment for Lignin Valorization and Ethanol Production

Rabelo

et al. 2021

ACS Sustainable Chem. Eng.

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“…Long et al [36] and Hou [22] reported the same behavior for sugar consumption, who used free cells of S. passalidarum at the initial concentration of 1.23 g/L. However, the effect of catabolite repression, characterized by the sequential consumption of glucose and xylose is reported in the literature for this microorganism [23,34,40,41]. Catabolite repression is observed in the metabolism of most microorganisms and is the phenomenon that occurs when the consumption of glucose present in the environment prevents the expression of genes that encode enzymes necessary for the metabolization of other sugars [36].…”

Section: Sbf With Immobilized and Free S Passalidarum Cellsmentioning

confidence: 99%

Immobilization of Spathaspora passalidarum NRRL Y-27907 in Calcium Alginate Aiming the Production of Second-Generation Ethanol

et al. 2021

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The immobilization of S. passalidarum in calcium alginate beads for second-generation ethanol production (E2G) was evaluated in a medium that simulated a hemicellulosic hydrolysate of sugarcane bagasse pretreated with diluted sulfuric acid in terms of sugars composition. Three sets of sequential batch fermentations (SBF) were carried out with free cells, or immobilized cells in high (HSC) and moderate (MSC) initial sugar concentration (120 and 70 g/L, respectively). SBF were characterized by five consecutive batches performed in shaker, at 30 °C and 110 rpm. Better results were observed for the SBF with immobilized cells in MSC medium when compared to HSC (Y'P/S of 0.27 ± 0.02 and 0.19 ± 0.03 g/g, respectively), in the second batch cycle. The value for YP/S in MSC was similar to the obtained with free cells (0.30 ± 0.02 to 0.33 ± 0.02 g/g). However, QP was lower for MSC, reaching 0.81 ± 0.04 g/L.h in in the second batch, while for free cells the QP varied from 1.06 ± 0.02 to 1.16 ± 0.22 g/L.h. A technique for determining the concentration of immobilized cells in the alginate beads was applied, which made it possible to determine the specific rates for the SBF performed. According to the results obtained, it was possible to demonstrate that S. passalidarum can be immobilized in calcium alginate and reuse through SBF, with performance similar to free cells, which can be a good strategy for fermentation of hemicellulosic hydrolysates.

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First‐ and second‐generation integrated process for bioethanol production: Fermentation of molasses diluted with hemicellulose hydrolysate by recombinant Saccharomyces cerevisiae

de Oliveira Pereira,

dos Santos,

Guimarães

et al. 2024

Biotech & Bioengineering

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The integration of first‐ (1G) and second‐generation (2G) ethanol production by adding sugarcane juice or molasses to lignocellulosic hydrolysates offers the possibility to overcome the problem of inhibitors (acetic acid, furfural, hydroxymethylfurfural and phenolic compounds), and add nutrients (such as salts, sugars and nitrogen sources) to the fermentation medium, allowing the production of higher ethanol titers. In this work, an 1G2G production process was developed with hemicellulosic hydrolysate (HH) from a diluted sulfuric acid pretreatment of sugarcane bagasse and sugarcane molasses. The industrial Saccharomyces cerevisiae CAT‐1 was genetically modified for xylose consumption and used for co‐fermentation of sucrose, fructose, glucose, and xylose. The fed‐batch fermentation with high cell density that mimics an industrial fermentation was performed at bench scale fermenter, achieved high volumetric ethanol productivity of 1.59 g L−1 h−1, 0.39 g g−1 of ethanol yield, and 44.5 g L−1 ethanol titer, and shown that the yeast was able to consume all the sugars present in must simultaneously. With the results, it was possible to establish a mass balance for the global process: from pretreatment to the co‐fermentation of molasses and HH, and it was possible to establish an effective integrated process (1G2G) with sugarcane molasses and HH co‐fermentation employing a recombinant yeast.

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A differential evolution approach to estimate parameters in a temperature-dependent kinetic model for second generation ethanol production under high cell density with Spathaspora passalidarum

Cited by 15 publications

References 52 publications

Effect of Protic Ionic Liquids in Sugar Cane Bagasse Pretreatment for Lignin Valorization and Ethanol Production

Effect of Protic Ionic Liquids in Sugar Cane Bagasse Pretreatment for Lignin Valorization and Ethanol Production

Immobilization of Spathaspora passalidarum NRRL Y-27907 in Calcium Alginate Aiming the Production of Second-Generation Ethanol

First‐ and second‐generation integrated process for bioethanol production: Fermentation of molasses diluted with hemicellulose hydrolysate by recombinant Saccharomyces cerevisiae

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