A Different Point of View of Glutathione Peroxidase: its Relationship to the Metabolic Changes Associated with Nutritional Management in Assaf ovine breed

Abstract

SummaryWe considered the role played by selenium-dependent glutathione peroxidase (GSH-Px) in relation to the metabolic changes due to nutritional management in a flock ofthe Assaf breed in Spain, housed in an intensive management system. Our objective was to examine the relationship between the nutritional management of this flock during different productive stages and GSH-Px. The modifications of ASAT and CK activities, closely connected to the pathological changes in GSH-Px values, were also recorded. The animals were divided into two groups: control and experimental ewes. Our results showed that neither physiological condition nor litter size had any influence on the GSH-Px values; the main modifications were due to the diet of these animals, especially if the diet was rieh in essential fatty acids. The evolution of ASAT and CK activities, and their relationship to GSH-Px and nutrition, is also described.Key Words: glutathione peroxidase, metabolism, ewe, pregnancy Zusammenfassung Titel der Arbeit: Ein anderer Aspekt der Glutathionperoxidase: Ihre Beziehung zu metabolischen Veränderungen im Zusammenhang mit dem Futterungsmanagement bei Assaf-Schafen Untersucht wurde die Bedeutung der Selen-abhängigen Glutathion-Peroxidase(GSH-Px) in Bezug auf füt-terungsbedingte Stoffwechselveränderungen in einer Herde von Assaf-Schafen unter intensiven Haltungsbedingungen. Fragestellung war ein möglicher Zusammenhang zwischen dem FUtterungsmanagement in verschiedenen Produktionsphasen und der GSH-Px-Aktivität. Desweiteren wurden Änderungen der AST-und CKAktivität berücksichtigt, welche mit der GSH-Px eng verknüpft sind. Die untersuchten Mutterschafe wurden in Kontroll-und Versuchsgruppe unterteilt. Die Ergebnisse zeigen, daß die GSH-Px-Werte weder durch den physiologischen Zustand noch durch die Zahl der Lämmer beeinflusst wurden. Die stärksten Veränderungen ergaben sich durch die Fütterung, vor allem durch Rationen mit hohem Gehalt an essentiellen Fettsäuren. Die Entwicklung der AST und CK-Aktivität sowie deren Beziehung zur GSH-Px werden ebenfalls beschrieben.

Values of plasma lipid hydroperoxides and total antioxidant status in healthy dairy cows: preliminary observations

“…This fact itself can justify the differences found in LOOH values between groups, although we cannot exclude the influence of diet at this moment on this parameter. In ruminant nutrition, this kind of diet, rich in essential fatty acids, has adverse effects like the increased conversion of polyunsaturated fatty acids, derived from essential fatty acids, to lipid peroxides (HUSVÉTH et al, 2000;CASTILLO et al, 2001b). But normally the body is protected by a wide range of antioxidant systems that neutralise harmful oxidants (NEMEC et al, 2000).…”

Effect of fasting on lipid metabolism and oxidative stability in fattening chicken fed a diet supplemented with organic selenium

“…Antioxidants protect biological molecules from damages occurring either in physiologic conditions or upon exposure to the action of oxidative compounds. Dietary antioxidants such as vitamins A, C and E, and selenium are an important component of antioxidative protection (Temple 2000, Castillo et al 2001, Kolodziej & Jacyno 2005, Balicka-Ramisz et al 2006. Selenium is taken to the body with food in its organic (selenomethionine, selenocysteine) or inorganic (selenates, selenites) form.…”

Erythrocyte potassium, sodium and glutathione concentrations and their relationship with reproduction, body weight and fleece weight traits in Awassi sheep

“…On the other hand, in a study performed on Black Bengal goats authors did not find correlation with reproductive traits and K e concentration (MANDAL et al, 1988). Concentration of GSH has also important role on some productivity traits (CASTILLO et al, 2001;BALICKA-RAMICZ et al, 2006). In the present experiment, GSH H type Awassi sheep had higher milk production capacity and lower fleece weight.…”

“…An integral component of the enzyme glutathione peroxidase is selenium. The physiological function and peroxidant role of this enzyme have been precisely determined (SZILAGYI et al, 1994;CASTILLO et al, 2001;KOLEDZIEJ et al, 2005). In some sheep breeds, levels of GSH show a distinct bimodal distribution and as potassium and sodium concentrations it is possible to divide animals into two groups according to their erythrocyte GSH concentrations.…”

“…GSH concentration is regulated by a pair of autosomal alleles; however this genetic effect is modulated by environmental and some other genetic factors. (CASTILLO et al, 2001;SWIDERSKA-KOLACZ et al, 2001). Several studies have been conducted to find out correlations between GSH concentration and different production/ reproduction parameters.…”