A dielectric theory of “multi-stratified shell” model with its application to a lymphoma cell

Irimajiri, Akihiko; Hanai, Toshihiko; Inouye, Akira

doi:10.1016/0022-5193(79)90268-6

Cited by 230 publications

(151 citation statements)

References 12 publications

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“…Bacteria and cells can be modeled to take account of their heterogeneous structures using the so-called multishell model. 15 Erythrocytes are usually discoid in shape, but when suspended in an electrolyte they often take the form of a spherical particle ͑about 7 m in diameter͒ and can be represented as a thin spherical membrane ͑i.e., a single shell͒ surrounding the cytoplasm. To represent cells such as leukocytes, which possess a nucleus, we require a three-shell model ͑first shell the plasma membrane; second shell the cytoplasm; and third shell the membrane envelope surrounding the nucleoplasm͒.…”

Section: ͑14͒mentioning

confidence: 99%

Review Article—Dielectrophoresis: Status of the theory, technology, and applications

2010

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A review is presented of the present status of the theory, the developed technology and the current applications of dielectrophoresis ͑DEP͒. Over the past 10 years around 2000 publications have addressed these three aspects, and current trends suggest that the theory and technology have matured sufficiently for most effort to now be directed towards applying DEP to unmet needs in such areas as biosensors, cell therapeutics, drug discovery, medical diagnostics, microfluidics, nanoassembly, and particle filtration. The dipole approximation to describe the DEP force acting on a particle subjected to a nonuniform electric field has evolved to include multipole contributions, the perturbing effects arising from interactions with other cells and boundary surfaces, and the influence of electrical double-layer polarizations that must be considered for nanoparticles. Theoretical modelling of the electric field gradients generated by different electrode designs has also reached an advanced state. Advances in the technology include the development of sophisticated electrode designs, along with the introduction of new materials ͑e.g., silicone polymers, dry film resist͒ and methods for fabricating the electrodes and microfluidics of DEP devices ͑photo and electron beam lithography, laser ablation, thin film techniques, CMOS technology͒. Around three-quarters of the 300 or so scientific publications now being published each year on DEP are directed towards practical applications, and this is matched with an increasing number of patent applications. A summary of the US patents granted since January 2005 is given, along with an outline of the small number of perceived industrial applications ͑e.g., mineral separation, micropolishing, manipulation and dispensing of fluid droplets, manipulation and assembly of micro components͒. The technology has also advanced sufficiently for DEP to be used as a tool to manipulate nanoparticles ͑e.g., carbon nanotubes, nano wires, gold and metal oxide nanoparticles͒ for the fabrication of devices and sensors. Most efforts are now being directed towards biomedical applications, such as the spatial manipulation and selective separation/enrichment of target cells or bacteria, high-throughput molecular screening, biosensors, immunoassays, and the artificial engineering of three-dimensional cell constructs. DEP is able to manipulate and sort cells without the need for biochemical labels or other bioengineered tags, and without contact to any surfaces. This opens up potentially important applications of DEP as a tool to address an unmet need in stem cell research and therapy.

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Section: ͑14͒mentioning

confidence: 99%

Review Article—Dielectrophoresis: Status of the theory, technology, and applications

2010

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“…1 The most common approach to characterize cells is to use a core-shell dielectric model. [2][3][4] For most biological cells consisting of cytoplasm and plasma membrane, spherical single-shell model has been widely used by researchers to represent blood cells 5,6 and cancer cells. [7][8][9][10][11] In some cases, such as plants and most micro-organisms where the cell is surrounded by a cell wall, two-shell dielectric models were used.…”

Section: Introductionmentioning

confidence: 99%

Dielectrophoretic capture voltage spectrum for measurement of dielectric properties and separation of cancer cells

Wu¹,

Yung²,

Lim³

2012

Biomicrofluidics

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In this paper, a new dielectrophoresis (DEP) method based on capture voltage spectrum is proposed for measuring dielectric properties of biological cells. The capture voltage spectrum can be obtained from the balance of dielectrophoretic force and Stokes drag force acting on the cell in a microfluidic device with fluid flow and strip electrodes. The method was demonstrated with the measurement of dielectric properties of human colon cancer cells . From the capture voltage spectrum, the real part of Clausius-Mossotti factor of HT-29 cells for different frequencies of applied electric field was obtained. The dielectric properties of cell interior and plasma membrane were then estimated by using single-shell dielectric model. The cell interior permittivity and conductivity were found to be insensitive to changes in the conductivity of the medium in which the cells are suspended, but the measured permittivity and conductivity of cell membrane were found to increase with the increase of medium conductivity. In addition, the measurement of capture voltage spectrum was found to be useful in providing the optimum operating conditions for separating HT-29 cells from other cells (such as red blood cells) using dielectrophoresis. V C 2012 American Institute of Physics. [http://dx

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“…Following the determination of the collection rate, the Clausius-Mossotti factor was fitted to the collection spectra was modelled using the multi-shell model 11 ; this method has been applied to yeast cells 12 , viruses 13 , cancer cells 9 and drug-resistant and drug-sensitive bacteria 10 , latex beads 14 Blue test showed that the visible cells were alive and hence not affected by the CuSO 4 ; modelling the data showed that the effective dielectric properties of these cells were the same as those of the untreated cells. However, some cells were observed exhibiting negative dielectrophoresis, which was not observed in the untreated cells and indicating that a population of the algae had changed in their dielectric properties significantly enough to prevent them collecting at the electrode edges.…”

Section: Resultsmentioning

confidence: 99%

Water quality test based on dielectrophoretic measurements of fresh water algae Selenastrum capricornutum

2003

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SummaryA method is described whereby dielectrophoresis of algal cells is used to perform rapid water quality analysis, specifically detecting the presence of CuSO 4 . The dielectric collection spectrum of the fresh water alga Selenastrum capricornutum was determined for a range of concentrations of CuSO 4 between 25 mg L -1 to 0.25 mg L -1 for exposure times of 15 minutes and 18 hours. In all cases increasing the concentration of CuSO 4 reduced cell collection, but a step reduction was observed in collection between 2mg L -1 and 5mg L -1 . This method has potential for forming a rapid, low-cost test for water quality with broad specificity and significantly reduced analysis time compared to current methods.

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A dielectric theory of “multi-stratified shell” model with its application to a lymphoma cell

Cited by 230 publications

References 12 publications

Review Article—Dielectrophoresis: Status of the theory, technology, and applications

Review Article—Dielectrophoresis: Status of the theory, technology, and applications

Dielectrophoretic capture voltage spectrum for measurement of dielectric properties and separation of cancer cells

Water quality test based on dielectrophoretic measurements of fresh water algae Selenastrum capricornutum

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