A Device for the Measurement of Rotor Temperature in the Air-Driven Ultracentrifuge

Ecker, Paul; Blum, Josef; Hiatt, C. W.

doi:10.1063/1.1741393

Cited by 9 publications

(3 citation statements)

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“…Systems have been developed that were based on electrical conductance [6,19], optical scanning [8], thermal infrared radiation [9,10], and radio telemetry [20]. Taking advantage of recent developments in integrated circuits, it has become possible to omit any information transfer from the spinning rotor, and instead store the measurements for later retrieval.…”

Section: Discussionmentioning

confidence: 99%

“…Unfortunately, a measurement of the sample solution temperature in a spinning rotor is non-trivial and many methods have been developed during the long history of AUC. These include devices based on calibrated thermocouples [1,5,6], methods based on the observation of the melting point of solids [7,8], radiometers [9,10], and thermochromic solutions [11]. A radiometer is currently implemented in the ProteomeLab analytical ultracentrifuge (Beckman Coulter, Indianapolis, IN).…”

Section: Introductionmentioning

confidence: 99%

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Improved measurement of the rotor temperature in analytical ultracentrifugation

Zhao

Balbo

Metger

et al. 2014

Analytical Biochemistry

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Sedimentation velocity is a classical method for measuring the hydrodynamic, translational friction coefficient of biological macromolecules. In a recent study, comparing various analytical ultracentrifuges, we have shown that external calibration of the scan time, radial magnification, and temperature are critically important for accurate measurements (Anal. Biochem., 2013, doi: 10.1016/j.ab.2013.05.011). To achieve accurate temperature calibration, we have introduced the use of an autonomous miniature temperature logging integrated circuit (Maxim Thermochron iButton ™) that can be inserted in an ultracentrifugation cell assembly and spun at low rotor speeds. In the present work, we developed an improved holder for the temperature sensor located in the rotor handle. This has the advantage of not reducing the rotor capacity and allows for a direct temperature measurement of the spinning rotor during high-speed sedimentation velocity experiments up to 60,000 rpm. We demonstrate the sensitivity of this approach by monitoring the adiabatic cooling due to rotor stretching during rotor acceleration, and the reverse process upon rotor deceleration. Based on this, we developed a procedure to approximate isothermal rotor acceleration for better temperature control.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Improved measurement of the rotor temperature in analytical ultracentrifugation

Zhao

Balbo

Metger

et al. 2014

Analytical Biochemistry

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“…These include devices based on calibrated thermocouples [52–54], infrared radiation [51,55], the melting point of solids [41,48,52], and the use of solutions with temperature-sensitive absorbance properties [49]. Radiometers are now used in the commercial instruments, and routine temperature verification by the manufacturer is performed by a post-centrifugal temperature analysis using a calibrated thermometer.…”

Section: Introductionmentioning

confidence: 99%

Improving the thermal, radial, and temporal accuracy of the analytical ultracentrifuge through external references

Ghirlando

Balbo

Piszczek

et al. 2013

Analytical Biochemistry

106

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Sedimentation velocity (SV) is a method based on first-principles that provides a precise hydrodynamic characterization of macromolecules in solution. Due to recent improvements in data analysis, the accuracy of experimental SV data emerges as a limiting factor in its interpretation. Our goal was to unravel the sources of experimental error and develop improved calibration procedures. We implemented the use of a Thermochron iButton® temperature logger to directly measure the temperature of a spinning rotor, and detected deviations that can translate into an error of as much as 10% in the sedimentation coefficient. We further designed a precision mask with equidistant markers to correct for instrumental errors in the radial calibration, which were observed to span a range of 8.6%. The need for an independent time calibration emerged with use of the current data acquisition software (Zhao et al., doi 10.1016/j.ab.2013.02.011) and we now show that smaller but significant time errors of up to 2% also occur with earlier versions. After application of these calibration corrections, the sedimentation coefficients obtained from eleven instruments displayed a significantly reduced standard deviation of ∼ 0.7 %. This study demonstrates the need for external calibration procedures and regular control experiments with a sedimentation coefficient standard.

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Ultrazentrifugen

Elias

1964

Chemie Ingenieur Technik

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A Device for the Measurement of Rotor Temperature in the Air-Driven Ultracentrifuge

Cited by 9 publications

References 0 publications

Improved measurement of the rotor temperature in analytical ultracentrifugation

Improved measurement of the rotor temperature in analytical ultracentrifugation

Improving the thermal, radial, and temporal accuracy of the analytical ultracentrifuge through external references

Ultrazentrifugen

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