A device for the liberation and determination of 14CO2

Schadewaldt, Peter; Förster, Malte; Münch, Ulrich; Staib, W.

doi:10.1016/0003-2697(83)90026-x

Cited by 14 publications

(4 citation statements)

References 27 publications

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“…were prepared essentially as described by Ruderman et al (1971). Non-recirculating per-fusion with a medium containing 0.15 mM-sodium pyruvate was performed as described previously (Schadewaldt et al, 1983a). Under standard perfusion conditions, tracer doses of [1-14C]pyruvate (750-775 MBq/mmol) were infused to give final arterial specific radioactivities of 1.3-1.5Bq/nmol.…”

Section: Methodsmentioning

confidence: 99%

“…Lactate (Gutmann & Wahlefeld, 1974), pyruvate (Czok & Lamprecht, 1974) and glycogen (Seifter et al, 1950) were determined by spectrophotometric methods. '4C02 production was measured as described previously (Schadewaldt et al, 1983a). 14CO2 release was calculated from 'the 14CO2 production on the basis of the specific-radioactivity of [1-14C]pyruvate in the perfusate.…”

Section: Methodsmentioning

confidence: 99%

“…The radioactivity in these metabolites was determined simultaneously (Odessey & Goldberg, 1979). Samples were incubated with an equal volume of 2 M-citric acid/3 M-H202 solution and 14CO2 was measured as described elsewhere (Schadewaldt et al, 1983a). In a number of experiments, lactate was isolated from HC104 extracts of the effluent.…”

Section: Methodsmentioning

confidence: 99%

“…The sources for the radioactive and unlabelled material were as in Schadewaldt et al (1983a). Results are means ± S.D.…”

Section: Methodsmentioning

confidence: 99%

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Evidence for the compartmentation of pyruvate metabolism in perfused rat skeletal muscle

Schadewaldt¹,

Münch²,

Staib³

1983

Biochemical Journal

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In rat hindlimbs perfused with [1-14C]pyruvate and 5 mM-dichloroacetate, the calculated apparent rate of pyruvate decarboxylation was decreased with increasing perfusate pyruvate concentrations. However, in the absence of dichloroacetate the apparent rate of decarboxylation increased under these conditions. Dichloroacetate enhanced [1-14C]pyruvate uptake, but decreased the specific radioactivity of effluent lactate. Glycogen metabolism remained unaffected. The results were not consistent with a common pyruvate pool, but provide evidence for the compartmentation of pyruvate metabolism.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

“…The sources for the radioactive and unlabelled material were as in Schadewaldt et al (1983a). Results are means ± S.D.…”

Section: Methodsmentioning

confidence: 99%

See 2 more Smart Citations

Evidence for the compartmentation of pyruvate metabolism in perfused rat skeletal muscle

Schadewaldt¹,

Münch²,

Staib³

1983

Biochemical Journal

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Duration of Improved Muscle Glucose Uptake After Acute Exercise in Obese Zucker Rats

et al. 1993

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Skeletal muscle is insulin resistant in the obese Zucker rat. Endurance training reduces muscle insulin resistance, but the effects of a single acute exercise session on muscle insulin resistance in the obese Zucker rat are unknown. Therefore, insulin responsiveness of muscle glucose uptake was measured in 15-week-old obese rats either 1, 48, or 72 hours after two hours of intermittent exercise (30:30 min; work:rest). Hindlimbs of sedentary lean (LS) and obese (OS) rats and exercised obese (OE) rats were perfused after a 10-hour fast under both basal (0 mU x ml(-1)) and maximal (20 mU x ml(-1)) insulin concentrations to measure net glucose uptake. Insulin responsiveness of net glucose uptake was significantly reduced in OS compared to LS (8.5 +/- 1.6 vs 15.3 +/- 2.0 micromol x g(-1) x h(-1), respectively). Compared to OS, insulin responsiveness of net glucose uptake was significantly increased by 56% and 80% at 1 hour and 48 hours after acute exercise. However, 72 hours after acute exercise, the increased insulin responsiveness of net glucose uptake was no longer evident. These results indicate that improved responsiveness of muscle glucose uptake persists for at least 48 hours after two hours of acute intermittent exercise in 15-week-old obese Zucker rats.

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Method for the estimation of the intracellular dilution of [1-14C]pyruvate in rat hindlimb perfusion studies

et al. 1984

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A device for the liberation and determination of 14CO2

Cited by 14 publications

References 27 publications

Evidence for the compartmentation of pyruvate metabolism in perfused rat skeletal muscle

Evidence for the compartmentation of pyruvate metabolism in perfused rat skeletal muscle

Duration of Improved Muscle Glucose Uptake After Acute Exercise in Obese Zucker Rats

Method for the estimation of the intracellular dilution of [1-14C]pyruvate in rat hindlimb perfusion studies

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