A device for the electrostatic precipitation of bacteria and fungus spores upon culture plates

Rooks, Roland

doi:10.1016/0021-8707(48)90053-7

Cited by 4 publications

(2 citation statements)

References 4 publications

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“…Subsequently, a large number of studies were carried out to investigate the airborne microbial agents (Keegan 1918;Tolman et al 1919;Greenburg and Smith 1922;Rooks 1948;Rosenstern 1948;Cown et al 1957). These studies used various samplers, including an all-glass impinger (Greenburg and Smith 1922), a device utilizing the electrical force for collecting bacteria and fungus (Rooks 1948), and a critical-orifice liquid Impinger (Cown et al 1957).…”

Section: Overviewmentioning

confidence: 99%

“…These studies used various samplers, including an all-glass impinger (Greenburg and Smith 1922), a device utilizing the electrical force for collecting bacteria and fungus (Rooks 1948), and a critical-orifice liquid Impinger (Cown et al 1957). A key moment for the bioaerosol research came in 1933 when fungal spores, algae, and diatoms were found in the air samples collected during the flight from Maine to Denmark by the US Department of Agriculture.…”

Section: Overviewmentioning

confidence: 99%

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Bioaerosol Science, Technology, and Engineering: Past, Present, and Future

Shen

et al. 2011

Aerosol Science and Technology

134

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Section: Overviewmentioning

confidence: 99%

Section: Overviewmentioning

confidence: 99%

Bioaerosol Science, Technology, and Engineering: Past, Present, and Future

Shen

et al. 2011

Aerosol Science and Technology

134

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The value and limitations of mold surveys by the culture plate method

Rooks

Shapiro

1958

Journal of Allergy

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Enhancing Bioaerosol Sampling by Andersen Impactors Using Mineral-Oil-Spread Agar Plate

Wei

et al. 2013

PLoS ONE

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As a bioaerosol sampling standard, Andersen type impactor is widely used since its invention in 1950s, including the investigation of the anthrax attacks in the United States in 2001. However, its related problems such as impaction and desiccation stress as well as particle bounce have not been solved. Here, we improved its biological collection efficiencies by plating a mineral oil layer (100 µL) onto the agar plate. An Andersen six-stage sampler and a BioStage impactor were tested with mineral-oil-spread agar plates in collecting indoor and outdoor bacterial and fungal aerosols. The effects of sampling times (5, 10 and 20 min) were also studied using the BioStage impactor when sampling environmental bioaerosols as well as aerosolized Bacillus subtilis (G+) and Escherichia coli (G-). In addition, particle bounce reduction by mineral-oil-plate was also investigated using an optical particle counter (OPC). Experimental results revealed that use of mineral-oil-spread agar plate can substantially enhance culturable bioaerosol recoveries by Andersen type impactors (p-values<0.05). The recovery enhancement was shown to depend on bioaerosol size, type, sampling time and environment. In general, more enhancements (extra 20%) were observed for last stage of the Andersen six-stage samplers compared to the BioStage impactor for 10 min sampling. When sampling aerosolized B. subtilis, E. coli and environmental aerosols, the enhancement was shown to increase with increasing sampling time, ranging from 50% increase at 5 min to ∼100% at 20 min. OPC results indicated that use of mineral oil can effectively reduce the particle bounce with an average of 66% for 10 min sampling. Our work suggests that enhancements for fungal aerosols were primarily attributed to the reduced impaction stress, while for bacterial aerosols reduced impaction, desiccation and particle bounce played major roles. The developed technology can readily enhance the agar-based techniques including those high volume portable samplers for bioaerosol monitoring.

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A device for the electrostatic precipitation of bacteria and fungus spores upon culture plates

Cited by 4 publications

References 4 publications

Bioaerosol Science, Technology, and Engineering: Past, Present, and Future

Bioaerosol Science, Technology, and Engineering: Past, Present, and Future

The value and limitations of mold surveys by the culture plate method

Enhancing Bioaerosol Sampling by Andersen Impactors Using Mineral-Oil-Spread Agar Plate

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