A developmental and histochemical study of the female reproductive system in Ornithogalum caudatum Ait. using light and electron microscopy

Abstract

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Florogenesis and Female Gametophyte Development in <i>Allium cepa</i> L. cv. Krishnapuram

“…The existence of short lived antipodal cells has also been reported in Chichoriumintybus [31] and Agave tequilana [32]. In addition to that, the close contact of the egg apparatus with the micropyle is highly similar to what was observed in the ovular apparatus of Ornithogalum caudatum [33]. Moreover, as observed in many other angiosperms, the egg cell possesses a highly dense nucleus placed towards the chalazal extreme of the embryo sac [34].…”

OVULE DEVELOPMENT IN ORNITHOGALUM CAUDATUM (LILIACEAE) WITH A REVIEW OF SELECTED PAPERS ON ANGIOSPERM REPRODUCTION

“…It therefore is not surprising to find starch stored in the nucellus of Ornithogalum ovules. What is surprising, however, is the lack of lipid reserves in Ornithogalum nucelli because lipids occur so abundantly as storage material elsewhere in the gynoecium (Tilton, 1978). We have no explanation for this except to speculate that storage of starch here may be more efficient.…”

“…Tissues were processed in glutaraldhyde and OSO4 buffered with sodium cacodylate, triple-fixed with unbuflFered uranyl acetate, and embedded in Luft's epon (Luft, 1961). Sections for LM were stained with either Aniline blue black (total proteins; Fisher, 1968), Napthol black (total proteins; Tilton, 1978) or Methylene blue-azure II (nucleic acids; Humphrey and Pittman, 1974) and counterstained with basic fuchsin (carbonates; Humphrey and Pittman, 1974). Tissues for scanning electron microscopy (SFM) were cryofractured in liquid Ng, critical point dried with COg, and then coated with carbon and gold.…”

OVULE DEVELOPMENT IN ORNITHOGALUM CAUDATUM (LILIACEAE) WITH A REVIEW OF SELECTED PAPERS ON ANGIOSPERM REPRODUCTION

“…Sections were stained with either Aniline blue black (total proteins; Fisher, 1968), Napthol black (total proteins; Tilton, 1978), or Methylene blue-azure II (nucleic acids; Humphrey and Pittman, 1974) and counterstained with basic fuchsin (carbohydrates; Humphrey and Pittman, 1974). F'ollowing staining, slides were rinsed with double-distilled water, air dried at room temperature, and immersed in xylene overnight.…”