A developed high-performance thin-layer chromatography method for the determination of baicalin in Oroxylum indicum L. and its antioxidant activity

Abstract

A simple, accurate, precise, and specific high-performance thin-layer chromatography (HPTLC) method for the quantitative determination and validation of baicalin in different extracts of Oroxylum indicum has been developed for the first time. The mobile phase of acetone‒ethyl acetate‒water‒formic acid (2:10:0.5:0.5, V/V ) was used for achieving good separation. Densitometric determination was carried out at 318 nm. The calibration curves were found to be linear in the range between 200 and 1000 ng per spot. During the analysis, the ethanolic extract of O. indicum showed higher content of baicalin than acetone, DMSO, and DMF extracts. Further, the antioxidant potential of different extracts of O. indicum were assessed with the 2,2-diphenyl-1-picrylhydrazyl (DPPH) method. The developed method of HPTLC was validated for specificity, accuracy, precision and linearity. The ethanolic extract has unveiled significant antioxidant activity with a percentage inhibition of 67.34%. Graphic Abstract

Nanotransethosomes for enhanced transdermal delivery of mangiferin against rheumatoid arthritis: formulation, characterization, invivo pharmacokinetic and pharmacodynamic evaluation

“…Mangiferin (MNF) is a natural xanthanoid chiefly present in Mangifera indica, Iris unguicularis, Salacia chinensis, Cyclopia genistoides, Anemarrhena asphodeloides , and Bombax ceiba (Khare et al., 2016 ; Tang et al., 2014 ). MNF has been copiously used in myriads of therapeutic fields because of its profuse pharmacological profile including antidiabetic, antibacterial, antiarthritic, antitumor, antiviral, anti-inflammatory, antitumor, immunomodulatory, lipometabolism regulating, hepatoprotective, cardioprotective, anti hyperuricemic, antiaging, antioxidative, neuroprotective, antipyretic, and analgesic activity MNF evince promising antiarthritic potential by virtue of its anti-oxidant, anti-inflammatory and analgesic property (Adin et al., 2022a , 2022b , 2022c, 2022d; Adin et al, 2023 ; Gupta et al., 2022a , 2022b , 2022c , 2022d, 2022e ). Luczkiewicz et.…”

Spanlastics as a Potential Approach for Enhancing the Nose-To-Brain Delivery of Piperine: In Vitro Prospect and In Vivo Therapeutic Efficacy for the Management of Epilepsy

“…Piperine (PIP) is an alkaloid that is chiefly present in plants: Piper nigrum , Piper longum , and Piper officinarum [ 9 ]. PIP has been copiously explored in diverse therapeutic fields due to its multifaceted biological profile that includes antihypertensive, antioxidant, antipyretic, antitumor, anti-asthmatics, analgesic, anti-inflammatory, antidiarrheal, anxiolytic, hepato-protective, antibacterial, antispasmodic, immunomodulatory, antifungal, neuroprotective, antimutagenic, and antimetastatic activity [ 10 , 11 , 12 , 13 , 14 , 15 , 16 ]. PIP manifests promising antiepileptic potential by dint of its antioxidant and neuroprotective potential [ 17 ].…”

QbD Based Extraction of Naringin from Citrus sinensis L. Peel and its Antioxidant Activity

“…[34] It is a labor, and time-saving method compared to other optimization methods and requires fewer experiments to assess multiple factors and their interactions. [24] For the Optimization of extraction parameters of various phytoconstituents like flavonoids from Vitis vinifera, [25] quercitrin from Herba Polygoni capitati, [26] karanjin from Pongamia pinnata, [27] embelin from Emblica ribes, [28] lupeol from Ficus racemosa, [29] carthamin from Carthamus tinctorious, [30] glycyrrhizic acid from Glycyrrhiza glabra, [31] atropine from Atropa balledona, [32] mangiferin from Mangifera indica, [37] piperine from Piper longum, [38] and baicalin from Oroxylum indicum, [33,35,36] researchers have exploited this technique.…”