A detailed restriction endonuclease site map of theZea mays plastid genome

Larrinua, Ignacio M.; Muskavitch, Karen M. T.; Gubbins, Earl J.; Bogorad, Lawrence

doi:10.1007/bf01578513

Cited by 78 publications

(61 citation statements)

References 26 publications

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“…The organelle genome of maize chloroplast is a 139-kb circular molecule of double-strand DNA [17] which occurs in many identical copies within the organelle [18]. A physical map of the maize genome, sites therein for restriction enzymes BamHI, EcoRI, PstI and SalI and their coordinates in the molecule have been published (171.…”

Section: Resultsmentioning

confidence: 99%

“…Bam6 (5.3 kb long) spans the junction of the inverted repeat (IRII) and the large single-copy region, while Bam17' (2.5 kb) lies contiguous to Bam6 in the large single-copy region of the maize chloroplast genome [17]. The known orientation of this ribosomal protein gene cluster in tobacco [4] and Marchuntin [3] indicated that Baml7' should carry rpS8 and rpLI4, while Bam6 should contain the beginning regions (i. e. 5' part) of the cluster.…”

Section: Resultsmentioning

confidence: 99%

“…1). They are located in the large single-copy region of this genome [17] on the restriction fragment designated Baml7'. The coordinates of the coding regions calculated from the data in Fig.…”

Section: Discussionmentioning

confidence: 99%

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Nucleotide sequence and linkage map position of the genes for ribosomal proteins L14 and S8 in the maize chloroplast genome

Markmann-Mulisch

Subramanian

1988

European Journal of Biochemistry

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The nucleotide sequence of a 1287-base-pair segment of the maize (Zea mays) chloroplast DNA, encoding chloroplast ribosomal proteins L14, S8 and the C-terminal part of L16, has been determined using the dideoxychain-termination method. These data from a monocot plant are compared to the corresponding data from a dicot and a lower plant and from two bacteria. The deduced amino acid sequence of maize chloroplast L14 shows 80%, 81%, 51% and 52% and that of S8 shows 75%, 58%, 39% and 38% sequence identity, respectively, to the correspondiny sequences of Nicotiana tabacum, Marchantia polymorpha, Bacillus stearothermophilus and Escherichiu coli. The starting map coordinates of rpLI4 and rpS8 in the physical map of the maize chloroplast DNA [Larrinua, I. M., Muskavitch, K. M. T., Plant Mol. Biol. 2, 129-1401 are 31.330 and 31.841. The gene order is rpL16-spacer-rpLI4-spacer-rpS8. Shine-Dalgarno sequences (GGA and AGGAGG) and computer-derived stem-loop structures of dyad symmetry are present in the spacers and the 3' downstream region of rpS8, respectively, but a chloroplast promoter-like sequence could not be detected suggesting that the latter might be located further upstream in this ribosomal protein gene cluster in maize chloroplast DNA.Chloroplasts and mitochondria contain specific translation systems which serve to synthesize the limited number of proteins encoded in the DNA of these organelles. Chloroplast ribosomes contain approximately 58 ribosomal proteins [l] whose genes are divided between the nuclear and chloroplast genomes [2]. Recent work on the complete nucleotide sequence of the chloroplast genomes of tobacco and Marchantia has revealed the coding sequences of approximately twenty ribosomal protein genes in the organelle DNA [3, 41. Additional work (e.g. [5, 61) is necessary to establish the functional standing of each of these putative chloroplast genes.Our laboratory has been working on subcloning, nucleotide sequencing and linkage mapping of the ribosomal protein genes in maize chloroplast DNA [7 -91 with the aim of determining experimentally the operon organization and promoter strengths of these chloroplast protein genes [9]. The clustering pattern of ribosomal protein genes in the chloroplast [3, 41 is significantly different from that in Escherichiu coli [lo]. There exist also some differences (e.g. presence/absence of S16 and L21 genes, single copy/two copies of the S19 gene) between monocot and dicot plants and between them and the lower plant Marchantia.In this paper we report the nucleotide sequence and linkage map position data for the chloroplast ribosomal protein genes rpLf4 and rpS8 from maize (Zea mays). These data (the first of this kind from a monocot plant) are compared to the corresponding data from two other plants and two eubacteria.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

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Nucleotide sequence and linkage map position of the genes for ribosomal proteins L14 and S8 in the maize chloroplast genome

Markmann-Mulisch

Subramanian

1988

European Journal of Biochemistry

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“…Probes for transcripts of PG32 (or psbA, coding for the QB protein), the gene for the large subunit for RuBP carboxylase, and the 16 S rRNA gene were pZmc427 (pBR322 combined with a 1.9 kb EcoRI-BamHI fragment from EcoO'), pZmc461 (pBR322 and a 0.58 kb PstI fragment from Bam 9), and pZmc532 (pBR322 and the 3.1 kb fragment Bam 13), respectively. A long region (2.5 kb) containing genes for the a subunit ofCF, and subunits I and III ofCFo (26) on the maize plastid chromosome of most of the fragments used here have been reported earlier ( 14).…”

Section: Methodsmentioning

confidence: 71%

Nuclear Gene-Regulated Expression of Chloroplast Genes for Coupling Factor One in Maize

Kobayashi¹,

Bogorad²,

Miles³

1987

Plant Physiol.

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In order to gain a better understanding of the interaction between the chloroplast and nuclear genomes in controlling the expression of plastid genes and the biosynthesis of chloroplast proteins, maize (Zea mays) Plastids of higher plants can differentiate into a number of forms including photosynthetically active chloroplasts, starchstoring amyloplasts, and carotenoid crystal-containing chloroplasts. Plastid metabolism requires the products of hundreds of genes but the plastid genome encodes many fewer. Most plastid proteins are products of the nuclear-cytoplasmic system; they must be imported from outside of the organelle. Genes for polypeptides of multimeric components of plastids including ribosomes, RuBP3 carboxylase/oxygenase, CF1 (coupling factor one for photophosphorylation), thylakoids, and others are dispersed in the nuclear and plastid genomes. Such dispersal of genes seems to be a feature of eukaryotic cell biology (2). Understanding the mechanisms for integration of nuclear and organelle gene expression, particularly for these multimeric components, is a central problem of eukaryotic cell biology.

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“…DNA fragments encoding Rubisco large subunit (rbcL), actin (mac), D2 (psbD), the 34-kD subunit of the OEC (oeel) and the 16-kD subunit of the OEC (oee3) were cloned from maize (13,24,26) and DNA encoding the Dl protein (psbA) was cloned from Amaranthus hybridus (9). rbcL, psbA, psbD, and mac DNA fragments were radioactively labeled by the random hexamer method and oeel and oee3 were labeled by in vitro generation of radioactively labeled RNA with a Stratagene RNA synthesis kit.…”

Section: Electron Transport Studiesmentioning

confidence: 99%

Characterization of the Expression of the Photosystem II-Oxygen Evolving Complex in C₄ Species of Flaveria

Ketchner¹,

Sayre²

1992

Plant Physiol.

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We have determined the levels of photosystem 11 activity and polypeptide abundance in whole leaves and isolated bundle sheath and mesophyll cells of C4, "C4-like," and C3 species of the genus Flaverla (Asteraceae). On a chlorophyll basis, the whole leaf levels of the DI, D2, and 34-kilodalton photosystem 11 polypeptides were similar for each Flaveria species. Photosystem II activity varied twofold, but was not correlated with photosynthetic type (C3 or C4). The bundle sheath cell levels of photosystem 11 activity and associated polypeptides in C4-like and C4 Flaveria species were approximately one-half those observed in mesophyll cells but equivalent to those in bundle sheath cells of the C3 species, Flaveria cronquistll. Analyses of the steady-state levels of transcripts encoding photosystem 11 polypeptides indicated that there were no differences in transcript abundance between mesophyll and bundle sheath cells of the C4 Flaveria species. This pattem was in contrast to the three-to tenfold higher levels of transcripts encoding photosystem 11 polypeptides in mesophyll versus bundle sheath cells of maize. It is apparent that the higher mesophyll cell to bundle sheath ratio of photosystem 11 polypeptides in C4-and C4-like species of Flaveria is the result of higher levels of photosystem 11 expression in mesophyll cells rather than lower levels of expression in bundle sheath cells.generation ofelevated CO2 concentrations in the BSC, leading to a reduction in photorespiratory activities (6).The tissue-specific expression of C3 and C4 cycle enzymes is determined by developmentally regulated processes as well as by environmental (light) signals and may be regulated at the transcriptional and/or posttranscriptional level (4,10,12,15,20,23,25,28). In addition, the expression of some enzymes, e.g. Rubisco, requires the coordinate synthesis and assembly of subunits encoded by two separate genomes (chloroplast and nuclear) (4,15,25,26,28).To date, the molecular characterization of the expression of multi-subunit enzyme complexes in C4 plants has largely focused on this two subunit enzyme complex, Rubisco (4,25,27). Another multi-subunit enzyme complex that is expressed in a tissue-specific manner in NADP-ME type C4 plants is 11,26,28). The PSII complex is composed of more than 10 polypeptides which are both nuclear and chloroplast encoded and is expressed predominantly in the MC ofNADP-ME enzyme type C4 plants (17,23,26). Studies on the localization and expression of the PSII activity and polypeptides in C4 plants have, however, been limited to monocots such as maize and sorghum. In this study we have characterized the pattern of expression of PSII activity and polypeptides in C3, "C4-like," and C4 species of Flaveria (dicot).A characteristic feature of C4 plants is the differentiation of the photosynthetic tissues ofleaves into two distinct cell types, MC3 and BSC. These cells contain different complements of enzymes active in photosynthetic CO2 fixation (5). For example, PEPCase, the C4 cycle enzyme that catalyz...

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A detailed restriction endonuclease site map of theZea mays plastid genome

Cited by 78 publications

References 26 publications

Nucleotide sequence and linkage map position of the genes for ribosomal proteins L14 and S8 in the maize chloroplast genome

Nucleotide sequence and linkage map position of the genes for ribosomal proteins L14 and S8 in the maize chloroplast genome

Nuclear Gene-Regulated Expression of Chloroplast Genes for Coupling Factor One in Maize

Characterization of the Expression of the Photosystem II-Oxygen Evolving Complex in C₄ Species of Flaveria

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A detailed restriction endonuclease site map of theZea mays plastid genome

Cited by 78 publications

References 26 publications

Nucleotide sequence and linkage map position of the genes for ribosomal proteins L14 and S8 in the maize chloroplast genome

Nucleotide sequence and linkage map position of the genes for ribosomal proteins L14 and S8 in the maize chloroplast genome

Nuclear Gene-Regulated Expression of Chloroplast Genes for Coupling Factor One in Maize

Characterization of the Expression of the Photosystem II-Oxygen Evolving Complex in C4 Species of Flaveria

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Characterization of the Expression of the Photosystem II-Oxygen Evolving Complex in C₄ Species of Flaveria