A detailed quantitative outcome measure of glycosaminoglycans in human articular cartilage for cell therapy and tissue engineering strategies

Kuiper, Nicola J.; Sharma, Aarti

doi:10.1016/j.joca.2015.07.011

Cited by 36 publications

(39 citation statements)

References 36 publications

(67 reference statements)

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“…GAGs in articular cartilage change as a function of development, age and disease [16][17][18][19][20][21][22][23] . In adult cartilage, 50-80% of GAGs are CS chains which are composed of glucuronic acid and N-acetylgalactosamine monosaccharides.…”

Section: Introductionmentioning

confidence: 99%

“…In this age range, CS chains are ~25kD and almost entirely terminated with a 4-sulphated N-acetylgalactosamine 18 . During adulthood, CS chains tend to have more C6S than C4S, the chains are shorter at ~16kD, and they are terminated with a 4-or 6-sulphated N-acetylgalactosamine 8,22 , or a 4,6-sulphated N-acetylgalactosamine 18 19 . KS chains also demonstrate an age-related increase in chain length 20 .…”

Section: Introductionmentioning

confidence: 99%

“…KS chains also demonstrate an age-related increase in chain length 20 . HA has been reported to account for 1-10% of GAGs in articular cartilage although higher values have been reported 8,[21][22][23] .…”

Section: Introductionmentioning

confidence: 99%

“…Immunological assessment of ACI biopsies 20,24 have defined the distribution of GAGs but these approaches are limited in characterising sulphation pattern or the real quantitation of GAGs. Fluorophore-Assisted Carbohydrate Electrophoresis (FACE) has evolved over the last decade to be a highly sensitive technique for profiling GAGs 8,22,[25][26][27] . Our lab has used FACE in a range of applications 7 including describing age-related changes in GAGs in a limited number of ACI biopsies 8 and through the zones of normal, healthy cartilage 22 .…”

Section: Introductionmentioning

confidence: 99%

“…Methods Biopsies were taken from one patient (25 years old) at 12 months and 20 months post ACI-treatment and from three normal cadavers (21,22 and 25 years old). Fluorophore-assisted carbohydrate electrophoresis (FACE) was used to quantitatively assess the individual glycosaminoglycans.…”

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confidence: 99%

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A case study: Glycosaminoglycan profiles of autologous chondrocyte implantation (ACI) tissue improve as the tissue matures

et al. 2017

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

mentioning

confidence: 99%

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A case study: Glycosaminoglycan profiles of autologous chondrocyte implantation (ACI) tissue improve as the tissue matures

et al. 2017

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Advances in Bioadhesive Hydrogels for Musculoskeletal Tissue Application

Zhang,

Chien,

Fan

et al. 2024

Adv Funct Materials

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The musculoskeletal system, which is responsible for weight‐bearing, movement, and organ protection, faces many disorders arising from injuries, diseases, and trauma that affect millions of people worldwide, resulting in a decreased quality of life and socioeconomic burden. Tissue engineering is at the forefront of current research on tissue regeneration and demonstrates great potential for musculoskeletal tissue repair. Among the numerous grafts available, adhesive hydrogels have demonstrated potential for tissue applications. Despite the surge in the development of bioadhesive hydrogel formulations in recent years, the absence of an evaluation protocol for their formulation has led to the emergence of numerous similar products that do not fully meet the clinical requirements for applicability in musculoskeletal tissue regeneration. This review aims to address this gap by first discussing the design considerations for an ideal bioadhesive hydrogel relevant to successful musculoskeletal tissue repair. By thoroughly reviewing recent research advances in bioadhesive hydrogels, with a particular focus on their applications in facilitating musculoskeletal tissue repair, improvements are proposed in the current evaluation criteria for the development of novel bioadhesive hydrogels for musculoskeletal tissue applications, and several key challenges and research directions for their implementation are summarized.

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Sweet but Challenging: Tackling the Complexity of GAGs with Engineered Tailor‐Made Biomaterials

Le Pennec,

Picart,

Vivès

et al. 2023

Advanced Materials

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Glycosaminoglycans (GAGs) play a crucial role in tissue homeostasis by regulating the activity and diffusion of bioactive molecules. Incorporating GAGs into biomaterials has emerged as a widely adopted strategy in medical applications, owing to their biocompatibility and ability to control the release of bioactive molecules. Nevertheless, immobilized GAGs on biomaterials can elicit distinct cellular responses compared to their soluble forms, underscoring the need to understand the interactions between GAG and bioactive molecules within engineered functional biomaterials. By controlling critical parameters such as GAG type, density, and sulfation, it becomes possible to precisely delineate GAG functions within a biomaterial context and to better mimic specific tissue properties, enabling tailored design of GAG‐based biomaterials for specific medical applications. However, this requires access to pure and well‐characterized GAG compounds, which remains challenging. This review focuses on different strategies for producing well‐defined GAGs and explores high‐throughput approaches employed to investigate GAG–growth factor interactions and to quantify cellular responses on GAG‐based biomaterials. These automated methods hold considerable promise for improving the understanding of the diverse functions of GAGs. In perspective, the scientific community is encouraged to adopt a rational approach in designing GAG‐based biomaterials, taking into account the in vivo properties of the targeted tissue for medical applications.

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A detailed quantitative outcome measure of glycosaminoglycans in human articular cartilage for cell therapy and tissue engineering strategies

Abstract: As an outcome measure, FACE offers the potential of a complete, detailed assessment of all GAGs and offers more information that the widely used 1,9-dimethylmethylene blue (DMMB) dye assay. FACE could be very useful in the evolving cartilage regeneration field.

Cited by 36 publications

References 36 publications

A case study: Glycosaminoglycan profiles of autologous chondrocyte implantation (ACI) tissue improve as the tissue matures

A case study: Glycosaminoglycan profiles of autologous chondrocyte implantation (ACI) tissue improve as the tissue matures

Advances in Bioadhesive Hydrogels for Musculoskeletal Tissue Application

Sweet but Challenging: Tackling the Complexity of GAGs with Engineered Tailor‐Made Biomaterials

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