A detailed analysis of biodegradable nanospheres by different techniques—A combined approach to detect particle sizes and size distributions

Augsten, Christian; Киселев, М. А.; Gehrke, Rainer; Hause, Gerd; Mäder, Karsten

doi:10.1016/j.jpba.2007.12.031

Cited by 17 publications

(9 citation statements)

References 23 publications

(26 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The explorable length-scale depends primarily on the employed light/radiation wavelength. The small-angle scattering of X-rays and neutrons typically resolves structures measuring 1 nm to 100 nm in size (14)(15)(16)(17)(18)(19). Such limited explorable size, the high cost of the involved equipment, and/or the scarcity of suitable radiation sources restricts applicability of such scattering techniques in the pharmaceutical field.…”

Section: Introductionmentioning

confidence: 99%

Turbidity Spectroscopy for Characterization of Submicroscopic Drug Carriers, Such as Nanoparticles and Lipid Vesicles: Size Determination

Elsayed

Cevc²

2011

Pharm Res

View full text Add to dashboard Cite

show abstract

Section: Introductionmentioning

confidence: 99%

Turbidity Spectroscopy for Characterization of Submicroscopic Drug Carriers, Such as Nanoparticles and Lipid Vesicles: Size Determination

Elsayed

Cevc²

2011

Pharm Res

View full text Add to dashboard Cite

show abstract

“…The size information obtained from AsFlFFF appeared to be very accurate, especially for nanoparticles smaller than 250–300 nm. For the size characterization of poly( d , l -lactide- co -glycolide) nanosuspensions, Augsten et al [72] used small-angle X-ray scattering, photon correlation spectroscopy, and AsFlFFF–MALS. AsFlFFF allowed easy detection of the maximum particle size.…”

Section: Biomacromoleculesmentioning

confidence: 99%

Application of flow field-flow fractionation for the characterization of macromolecules of biological interest: a review

Qureshi

Kok

2010

Anal Bioanal Chem

View full text Add to dashboard Cite

An overview is given of the recent literature on (bio) analytical applications of flow field-flow fractionation (FlFFF). FlFFF is a liquid-phase separation technique that can separate macromolecules and particles according to size. The technique is increasingly used on a routine basis in a variety of application fields. In food analysis, FlFFF is applied to determine the molecular size distribution of starches and modified celluloses, or to study protein aggregation during food processing. In industrial analysis, it is applied for the characterization of polysaccharides that are used as thickeners and dispersing agents. In pharmaceutical and biomedical laboratories, FlFFF is used to monitor the refolding of recombinant proteins, to detect aggregates of antibodies, or to determine the size distribution of drug carrier particles. In environmental studies, FlFFF is used to characterize natural colloids in water streams, and especially to study trace metal distributions over colloidal particles. In this review, first a short discussion of the state of the art in instrumentation is given. Developments in the coupling of FlFFF to various detection modes are then highlighted. Finally, application studies are discussed and ordered according to the type of (bio) macromolecules or bioparticles that are fractionated.

show abstract

“…When combined with various detectors, AF4 offers the possibility to simultaneously obtain a number of characterization parameters of the sample in a single measurement. So far AF4 has successfully been used for characterization of polymers, biopolymers, nanoparticles, colloidal suspensions, delivery systems, lipoplexes, polyelectrolyte complexes …”

Section: Introductionmentioning

confidence: 99%

Asymmetric Flow Field-Flow Fractionation Investigation of Magnetopolyplexes

Haladjova

Rangelov

Geisler

et al. 2015

Macromol. Chem. Phys.

View full text Add to dashboard Cite

Asymmetric flow field‐flow fractionation (AF4) is an analytical separation technique capable of providing information on different parameters of complex systems in a single measurement. Although it shows promise to be an important tool in the field of gene therapy, AF4 has only occasionally been used to study gene delivery vectors such as polyplexes. Here, AF4 is applied to investigate novel vector systems based on hybrid polymer–magnetic micelles, magnetopolyplexes. These are multicomponent systems, composed of small magnetic nanoparticles loaded in cationic block copolymer micelles that are complexed with DNA; they exhibit enhanced transfection efficiency in the presence of a magnetic field. It is demonstrated that the application of AF4 to these systems provides complementary information related to the fractionation of the samples, the disintegration of the vectors, the presence of fractions of small particles, unbound micelles, and DNA, and the composition of the magnetopolyplexes, which can hardly be obtained by conventional methods.

show abstract

A detailed analysis of biodegradable nanospheres by different techniques—A combined approach to detect particle sizes and size distributions

Cited by 17 publications

References 23 publications

Turbidity Spectroscopy for Characterization of Submicroscopic Drug Carriers, Such as Nanoparticles and Lipid Vesicles: Size Determination

Turbidity Spectroscopy for Characterization of Submicroscopic Drug Carriers, Such as Nanoparticles and Lipid Vesicles: Size Determination

Application of flow field-flow fractionation for the characterization of macromolecules of biological interest: a review

Asymmetric Flow Field-Flow Fractionation Investigation of Magnetopolyplexes

Contact Info

Product

Resources

About