A designer biomimetic vector with a chimeric architecture for targeted gene transfer

Wang, Yuhua; Mangipudi, Sriramchandra Sastry; Canine, Brenda; Hatefi, Arash

doi:10.1016/j.jconrel.2009.03.005

Cited by 48 publications

(64 citation statements)

References 25 publications

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“…Nevertheless GALA has been utilized in several multi-functional systems as a discrete endosomal-disrupting motif. For example, the GALA peptide was incorporated into a biomimetic vector that also had four repeats of histone proteins to condense DNA, a targeting motif for HER2 and a cathepsin substrate that acts as an intracellular cleavage site (61) . Studies demonstrated that positioning GALA on the N-terminus of the multifunctional vector ensured fusogenic amphipathic activity.…”

Section: Endosomal Escapementioning

confidence: 99%

Designer peptide delivery systems for gene therapy

Loughran

McCrudden

McCarthy

2015

European Journal of Nanomedicine

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: Gene therapy has long been hailed as a revolutionary approach for the treatment of genetic diseases. The enthusiasm that greeted the harnessing of viruses for therapeutic DNA delivery has been tempered by concerns over safety. These concerns led to the development of alternative strategies for nucleic acid delivery to cells. One such strategy is the utilization of cationic peptides for the condensation of therapeutic DNA for delivery to its target. However, success of DNA as a therapy relies on its delivery to the nucleus of target cells, a process that is complicated by the many hurdles encountered following systemic administration. Non-viral peptide gene delivery strategies have sought inspiration from viruses in order to retain DNA delivering potency, but limit virulence. This review summarizes the progression of peptide-based DNA delivery systems, from rudimentary beginnings to the recent development of sophisticated multi-functional vectors that comprise distinct motifs with dedicated barrier evasion functions. The most promising peptides that achieve cell membrane permeabilization, endosomal escape and nuclear delivery are discussed.

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Section: Endosomal Escapementioning

confidence: 99%

Designer peptide delivery systems for gene therapy

Loughran

McCrudden

McCarthy

2015

European Journal of Nanomedicine

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“…The fusion vector was equipped with a HER2 targeting affibody, a DNA condensing motif derived from human histone H2A, and the fusogenic peptide GALA [120,121]. The vector in combination with SR39 and GCV showed 80% toxicity to SKOV-3 cancer cells in vitro.…”

Section: Entry Targeting: Vector-mediated Suicide Gene Therapymentioning

confidence: 99%

Gene-Directed Enzyme Prodrug Cancer Therapy

Karjoo

Ganapathy

Hatefi

2014

Gene Therapy of Cancer

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“…Generally, DBVs possess a DNA-condensing motif (DCM), endosomal disruption motif (EDM), nuclear localisation motif (NLS) and targeting motif (TP) [15,135].…”

Section: Designer Biomimetic Vectorsmentioning

confidence: 99%

Delivery of Nucleic Acids for Cancer Gene therapy: Overcoming extra- and intra-cellular Barriers

2016

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The therapeutic potential of cancer gene therapy has been limited by the difficulty of delivering genetic material to target sites. Various biological and molecular barriers exist which need to be overcome before effective non-viral delivery systems can be applied successfully in oncology.Herein, various barriers are described and strategies to circumvent such obstacles are discussed, considering both the extracellular and intracellular setting. Development of multifunctional delivery systems holds much promise for the progression of gene delivery, and a growing body of evidence supports this approach involving rational design of vectors, with a unique molecular architecture. In addition, the potential application of composite gene delivery platforms is highlighted which may provide an alternative delivery strategy to traditional systemic administration.

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A designer biomimetic vector with a chimeric architecture for targeted gene transfer

Cited by 48 publications

References 25 publications

Designer peptide delivery systems for gene therapy

Designer peptide delivery systems for gene therapy

Gene-Directed Enzyme Prodrug Cancer Therapy

Delivery of Nucleic Acids for Cancer Gene therapy: Overcoming extra- and intra-cellular Barriers

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