A designed peptide-based vaccine to combat Brucella melitensis, B. suis and B. abortus: Harnessing an epitope mapping and immunoinformatics approach

Tarrahimofrad, Hossein; Zamani, Javad; Hamblin, Michael R.; Darvish, Maryam; Mirzaei, Hamed

doi:10.1016/j.biopha.2022.113557

Cited by 9 publications

(5 citation statements)

References 117 publications

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“…These advancements have also facilitated the development of the novel vaccines creation approach, enabling the vaccines targeting a broad spectrum of bacterial and viral infections. Our prior research, has demonstrated that multi-epitope peptide vaccines can use more diverse and higher effective factors against infectious agents compared to vaccines based on single epitopes [ 16 , 30 ]. In line with this approach, the present study concentrated on multiepitope subunit vaccines.…”

Section: Discussionmentioning

confidence: 99%

“…Reverse vaccinology is a recent breakthrough that, in addition to the conventional and laborious vaccine creation approaches, integrates immunogenomics and bioinformatics to quickly generate unique multi-epitope-based subunit vaccines. This approach offers the potential to expedite the vaccine development process [ 15 , 16 ]. Today, there are extensive studies based on the use of bioinformatics and immunoinformatics tools to design new-generation vaccines [ 17 – 23 ].…”

Section: Introductionmentioning

confidence: 99%

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A computational approach to design a multiepitope vaccine against H5N1 virus

Dashti,

Raisi,

Pourali

et al. 2024

Virol J

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Since 1997, highly pathogenic avian influenza viruses, such as H5N1, have been recognized as a possible pandemic hazard to men and the poultry business. The rapid rate of mutation of H5N1 viruses makes the whole process of designing vaccines extremely challenging. Here, we used an in silico approach to design a multi-epitope vaccine against H5N1 influenza A virus using hemagglutinin (HA) and neuraminidase (NA) antigens. B-cell epitopes, Cytotoxic T lymphocyte (CTL) and Helper T lymphocyte (HTL) were predicted via IEDB, NetMHC-4 and NetMHCII-2.3 respectively. Two adjuvants consisting of Human β-defensin-3 (HβD-3) along with pan HLA DR-binding epitope (PADRE) have been chosen to induce more immune response. Linkers including KK, AAY, HEYGAEALERAG, GPGPGPG and double EAAAK were utilized to link epitopes and adjuvants. This construct encodes a protein having 350 amino acids and 38.46 kDa molecular weight. Antigenicity of ~ 1, the allergenicity of non-allergen, toxicity of negative and solubility of appropriate were confirmed through Vaxigen, AllerTOP, ToxDL and DeepSoluE, respectively. The 3D structure of H5N1 was refined and validated with a Z-Score of − 0.87 and an overall Ramachandran of 99.7%. Docking analysis showed H5N1 could interact with TLR7 (docking score of − 374.08 and by 4 hydrogen bonds) and TLR8 (docking score of − 414.39 and by 3 hydrogen bonds). Molecular dynamics simulations results showed RMSD and RMSF of 0.25 nm and 0.2 for H5N1-TLR7 as well as RMSD and RMSF of 0.45 nm and 0.4 for H5N1-TLR8 complexes, respectively. Molecular Mechanics Poisson-Boltzmann Surface Area (MM/PBSA) confirmed stability and continuity of interaction between H5N1-TLR7 with the total binding energy of − 29.97 kJ/mol and H5N1-TLR8 with the total binding energy of − 23.9 kJ/mol. Investigating immune response simulation predicted evidence of the ability to stimulate T and B cells of the immunity system that shows the merits of this H5N1 vaccine proposed candidate for clinical trials.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

A computational approach to design a multiepitope vaccine against H5N1 virus

Dashti,

Raisi,

Pourali

et al. 2024

Virol J

Self Cite

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“…The modified LPS with a defective core purified from Brucella carrying a mutated wadC gene potentiated cytokine secretion, representing a potential for vaccine development ( Conde-Álvarez et al., 2012 ; Conde-Álvarez et al., 2013 ; Zhao et al., 2018 ). Based on the TLR4, some groups design the multi-epitope vaccine candidates against Brucella ( Li, M. et al., 2022 ; Tarrahimofrad et al., 2022 ; Jalal et al., 2023 ; Malik et al., 2023 ; Yin et al., 2023 ). B. abortus Omp16 lipoprotein would be able to induce a protective immune response via a TLR4-dependent manner and is a promising self-adjuvanting vaccine against brucellosis ( Pasquevich et al., 2010 ).…”

Section: Role Of Tlrs In Brucella Infectionmentioning

confidence: 99%

Brucella infection and Toll-like receptors

Yu,

Gu,

Wang

et al. 2024

Front. Cell. Infect. Microbiol.

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Brucella consists of gram-negative bacteria that have the ability to invade and replicate in professional and non-professional phagocytes, and its prolonged persistence in the host leads to brucellosis, a serious zoonosis. Toll-like receptors (TLRs) are the best-known sensors of microorganisms implicated in the regulation of innate and adaptive immunity. In particular, TLRs are transmembrane proteins with a typical structure of an extracellular leucine-rich repeat (LRR) region and an intracellular Toll/interleukin-1 receptor (TIR) domain. In this review, we discuss Brucella infection and the aspects of host immune responses induced by pathogens. Furthermore, we summarize the roles of TLRs in Brucella infection, with substantial emphasis on the molecular insights into its mechanisms of action.

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“…By releasing IFN-γ, CD4 cells can promote neutrophil infiltration in infected areas and assist T cells in producing CXC chemokines. The protective response against Brucella is enhanced by stimulation of CD4+ and CD8+ T cells, DCs, macrophages (M), and inflammatory cytokines (IFN-γ and IFN) (Tarrahimofrad et al 2022).…”

Section: Immune Response To Brucella Sppmentioning

confidence: 99%

“…New strategies are now being researched to enhance the immune response against brucellosis because live attenuated bacterial vaccines prepared with antibiotic treatment have been shown to have negative effects. Therefore, the focus of current research is disease control by Brucella vaccination (Tarrahimofrad et al 2022).…”

Section: Introductionmentioning

confidence: 99%

Cytokine Response in Brucella Abortus Bovine Infection: Literature Review

Rahmawati¹,

Destiawan²,

Ningtyas³

et al. 2023

JBP

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Brucella Abortus infection in cattle is an infection that has a strategic effect on society, especially the economic sector. This disease is caused by gram-negative bacteria, namely Brucella abortion. Treatment of Brucella Abortus Infection in cattle is still not effective. Therapy based on the immune response caused by Brucella Abortus Infection, especially the cytokine response, is an effective choice considering that each type of cow has a different immune response in dealing with Brucella Abortus Infection. The purpose of writing this literature review is to identify cytokines that play a role in Brucella Abortus Infection in cattle. Method: automated PUBMED database search using keywords “Brucella Abortus Bovine”, “Cytokine”, and “Brucellosis”. Results: 11 articles that are relevant and meet the inclusion criteria to analyze the cytokines that play a role in Brucella Abortus Infection in cattle. Conclusion: Cytokines that play a role in Brucella Abortus infection in cattle are generally IL-1, IL-6, IL-12, IL-8, TNF-α, and IFN-γ.

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A designed peptide-based vaccine to combat Brucella melitensis, B. suis and B. abortus: Harnessing an epitope mapping and immunoinformatics approach

Cited by 9 publications

References 117 publications

A computational approach to design a multiepitope vaccine against H5N1 virus

A computational approach to design a multiepitope vaccine against H5N1 virus

Brucella infection and Toll-like receptors

Cytokine Response in Brucella Abortus Bovine Infection: Literature Review

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