A Descriptive Study of Lectin Histochemistry of the Placenta in Cattle following Inoculation of Neospora caninum

Dorsch, Matías A.; Yaniz, M.G. de; Fiorani, Franco; Hecker, Yanina P.; Odeón, Anselmo Carlos; Morrell, Eleonora; Campero, C.M.; Barbeito, CG; Moore, D.P.

doi:10.1016/j.jcpa.2018.10.172

Cited by 15 publications

(11 citation statements)

References 43 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Three of the 4 cows with different N. caninum genotype belonged to the same herd; therefore, genetic differences would not be attributed to the origin of the animals. In addition, 2 sequences obtained for MS10 (cow #2 and #6) revealed no identity with N. caninum cattle isolates obtained from Argentina ( 50 , 51 ) nor sequences reported on Nespora -aborted fetus DNA ( 52 ). The other MS10 sequence (cow #3) has already been reported ( 52 ).…”

Section: Discussionmentioning

confidence: 82%

Parasitemia and Associated Immune Response in Pregnant and Non-Pregnant Beef Cows Naturally Infected With Neospora caninum

et al. 2022

View full text Add to dashboard Cite

The aim of this longitudinal study was to characterize the parasitemia of Neospora caninum and the associated immunological parameters in naturally infected beef cows for 10 months. The following groups were established: Neospora caninum seropositive pregnant cows (+Preg, n = 7), seropositive non-pregnant cows (+Npreg, n = 7), seronegative pregnant cows (−Preg, n = 4), and seronegative non-pregnant cows (−Npreg, n = 4). Several samples were obtained for absolute and relative leukocyte counting, cytokines IL-10, IL-12, α-TNF, and γ-IFN quantification, specific IgG, IgG1, and IgG2 and avidity and N. caninum DNA molecular detection and quantification. The +Preg group had a higher frequency and concentration of N. caninum DNA in PBMC in the last third of pregnancy compared to +Npreg (p <0.05), with 22 and 8% of detection, respectively. Parasitemia correlated positively with IgG titers and negatively with IgG1/IgG2 ratio (p <0.05). On day 222 of the assay, the +Preg group had the lowest total leukocyte counting (p <0.05). The +Preg group had a higher concentration of IgG and higher avidity in the last third of gestation compared to +Npreg (p <0.05). Avidity correlated with total IgG and IgG2 (p <0.05). All +Preg cows gave birth to clinically healthy but seropositive calves before colostrum intake, therefore, the congenital transmission was 100% efficient. Only a complete N. caninum genotype from a placenta and a partial genotype from cow #3 of the group +Preg were achieved by multilocus microsatellite analysis. Overall, N. caninum parasitemia is frequent in seropositive beef cows during the last third of gestation. This correlates with higher antibody levels and a decrease in total leukocyte counting. The precise timing of the parasitemia may be used for diagnosis purposes and/or for design strategies to avoid vertical transmission. Further studies are needed to identify the immune molecular mechanisms that favor parasitemia during gestation in chronically infected cattle.

show abstract

Section: Discussionmentioning

confidence: 82%

Parasitemia and Associated Immune Response in Pregnant and Non-Pregnant Beef Cows Naturally Infected With Neospora caninum

et al. 2022

View full text Add to dashboard Cite

show abstract

“…Thus, infection of immortalized bovine trophoblasts with N. caninum induces the expression MMPs involved in ECM degradation (Cui et al, 2017;Horcajo et al, 2017). Additionally, infection by N. caninum alters the glycosylation pattern in the glycocalyx and apical cytoplasm of trophoblast (Dorsch et al, 2019; Figure 4A). The BNCs and the uterine epithelium also shows changes in sugars, including α-D-GalNAc, α-D-Man, β-D-Gal, α-D-Gluc, and NeuNac (Vonlaufen et al, 2004;Dorsch et al, 2019; Figure 4A).…”

Section: Adhesion Of N Caninum To the Placentamentioning

confidence: 96%

“…Modulation of gene expression in the host cell is also a mechanism for N. caninum to invade and cross the placental barrier (Horcajo et al, 2017;Dorsch et al, 2019). Thus, infection of immortalized bovine trophoblasts with N. caninum induces the expression MMPs involved in ECM degradation (Cui et al, 2017;Horcajo et al, 2017).…”

Section: Adhesion Of N Caninum To the Placentamentioning

confidence: 99%

“…Additionally, infection by N. caninum alters the glycosylation pattern in the glycocalyx and apical cytoplasm of trophoblast (Dorsch et al, 2019; Figure 4A). The BNCs and the uterine epithelium also shows changes in sugars, including α-D-GalNAc, α-D-Man, β-D-Gal, α-D-Gluc, and NeuNac (Vonlaufen et al, 2004;Dorsch et al, 2019; Figure 4A). The expression of the adhesion molecules E-selectin, P-selectin, VCAM-1, and ICAM-1 is also altered, possibly through paracrine activation, through the pro-inflammatory cascade activation, which involves the release of IL-1b, IL-8, and MCP-1 leading to cell activation and subsequent expression of the adhesion molecules (Taubert et al, 2006).…”

Section: Adhesion Of N Caninum To the Placentamentioning

confidence: 99%

See 1 more Smart Citation

Congenital Transmission of Apicomplexan Parasites: A Review

et al. 2021

View full text Add to dashboard Cite

Apicomplexans are a group of pathogenic protists that cause various diseases in humans and animals that cause economic losses worldwide. These unicellular eukaryotes are characterized by having a complex life cycle and the ability to evade the immune system of their host organism. Infections caused by some of these parasites affect millions of pregnant women worldwide, leading to various adverse maternal and fetal/placental effects. Unfortunately, the exact pathogenesis of congenital apicomplexan diseases is far from being understood, including the mechanisms of how they cross the placental barrier. In this review, we highlight important aspects of the diseases caused by species of Plasmodium, Babesia, Toxoplasma, and Neospora, their infection during pregnancy, emphasizing the possible role played by the placenta in the host-pathogen interaction.

show abstract

“…A marked change in a group of host factors at the feto-maternal interface in cows has been detected in several studies. Lectin-binding architectural patterns were disrupted by N. caninum (Nc-1) infection [199]. Reduction of pregnancy associated proteins 1 and 2 in pregnant cattle have been associated with a higher rate of Neospora-induced abortion in cows [200].…”

Section: Dendritic Cells and Macrophagesmentioning

confidence: 99%

From Signaling Pathways to Distinct Immune Responses: Key Factors for Establishing or Combating Neospora caninum Infection in Different Susceptible Hosts

Fereig

Nishikawa

2020

Pathogens

View full text Add to dashboard Cite

Neospora caninum is an intracellular protozoan parasite affecting numerous animal species. It induces significant economic losses because of abortion and neonatal abnormalities in cattle. In case of infection, the parasite secretes numerous arsenals to establish a successful infection in the host cell. In the same context but for a different purpose, the host resorts to different strategies to eliminate the invading parasite. During this battle, numerous key factors from both parasite and host sides are produced and interact for the maintaining and vanishing of the infection, respectively. Although several reviews have highlighted the role of different compartments of the immune system against N. caninum infection, each one of them has mostly targeted specific points related to the immune component and animal host. Thus, in the current review, we will focus on effector molecules derived from the host cell or the parasite using a comprehensive survey method from previous reports. According to our knowledge, this is the first review that highlights and discusses immune response at the host cell–parasite molecular interface against N. caninum infection in different susceptible hosts.

show abstract

A Descriptive Study of Lectin Histochemistry of the Placenta in Cattle following Inoculation of Neospora caninum

Cited by 15 publications

References 43 publications

Parasitemia and Associated Immune Response in Pregnant and Non-Pregnant Beef Cows Naturally Infected With Neospora caninum

Parasitemia and Associated Immune Response in Pregnant and Non-Pregnant Beef Cows Naturally Infected With Neospora caninum

Congenital Transmission of Apicomplexan Parasites: A Review

From Signaling Pathways to Distinct Immune Responses: Key Factors for Establishing or Combating Neospora caninum Infection in Different Susceptible Hosts

Contact Info

Product

Resources

About