A Demonstration of the Consistency of Maize Stover Pretreatment by Soaking in Aqueous Ammonia from Bench to Pilot-Scale

Athmanathan, Arun; Fallahi, Parisa; Lash, Terry; Trupia, Sabrina

doi:10.1007/s12155-018-9954-8

Cited by 3 publications

(1 citation statement)

References 47 publications

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“…Enzymatic hydrolysis at high solids loading increases the economic feasibility of the bioconversion process as it reduces the operating cost for hydrolysis and fermentation and minimizes energy requirements for other downstream processes, such as distillation [11,14,15]. However, as solids loading increases, the water available to facilitate the diffusion of enzymes into the biomass and the diffusion of sugars out into solution decreases [16][17][18]. Water availability is also important to reduce the viscosity of the slurry, thereby reducing the energy required for mixing.…”

Section: Introductionmentioning

confidence: 99%

A high solids field-to-fuel research pipeline to identify interactions between feedstocks and biofuel production

Chandrasekar

Joshi

Krieg

et al. 2021

Biotechnol Biofuels

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Background Environmental factors, such as weather extremes, have the potential to cause adverse effects on plant biomass quality and quantity. Beyond adversely affecting feedstock yield and composition, which have been extensively studied, environmental factors can have detrimental effects on saccharification and fermentation processes in biofuel production. Only a few studies have evaluated the effect of these factors on biomass deconstruction into biofuel and resulting fuel yields. This field-to-fuel evaluation of various feedstocks requires rigorous coordination of pretreatment, enzymatic hydrolysis, and fermentation experiments. A large number of biomass samples, often in limited quantity, are needed to thoroughly understand the effect of environmental conditions on biofuel production. This requires greater processing and analytical throughput of industrially relevant, high solids loading hydrolysates for fermentation, and led to the need for a laboratory-scale high solids experimentation platform. Results A field-to-fuel platform was developed to provide sufficient volumes of high solids loading enzymatic hydrolysate for fermentation. AFEX pretreatment was conducted in custom pretreatment reactors, followed by high solids enzymatic hydrolysis. To accommodate enzymatic hydrolysis of multiple samples, roller bottles were used to overcome the bottlenecks of mixing and reduced sugar yields at high solids loading, while allowing greater sample throughput than possible in bioreactors. The roller bottle method provided 42–47% greater liquefaction compared to the batch shake flask method for the same solids loading. In fermentation experiments, hydrolysates from roller bottles were fermented more rapidly, with greater xylose consumption, but lower final ethanol yields and CO2 production than hydrolysates generated with shake flasks. The entire platform was tested and was able to replicate patterns of fermentation inhibition previously observed for experiments conducted in larger-scale reactors and bioreactors, showing divergent fermentation patterns for drought and normal year switchgrass hydrolysates. Conclusion A pipeline of small-scale AFEX pretreatment and roller bottle enzymatic hydrolysis was able to provide adequate quantities of hydrolysate for respirometer fermentation experiments and was able to overcome hydrolysis bottlenecks at high solids loading by obtaining greater liquefaction compared to batch shake flask hydrolysis. Thus, the roller bottle method can be effectively utilized to compare divergent feedstocks and diverse process conditions.

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Section: Introductionmentioning

confidence: 99%

A high solids field-to-fuel research pipeline to identify interactions between feedstocks and biofuel production

Chandrasekar

Joshi

Krieg

et al. 2021

Biotechnol Biofuels

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Chemical and thermochemical methods on lignocellulosic biorefinery

Balan

Jin

Ubanwa

2020

Recent Advances in Bioconversion of Lignocellulose to Biofuels and Value-Added Chemicals Within the Biorefinery Concept

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Emerging trends in high-solids enzymatic saccharification of lignocellulosic feedstocks for developing an efficient and industrially deployable sugar platform

Baral

Kumar

Agrawal

2021

Critical Reviews in Biotechnology

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For the techno-commercial success of any lignocellulosic biorefinery, the cost-effective production of fermentable sugars for the manufacturing of bio-based products is indispensable. High-solids enzymatic saccharification (HSES) is a straightforward approach to developing an industrially deployable sugar platform.Economic incentives such as reduced capital and operational expenditure along with environmental benefits in the form of reduced effluent discharge make this strategy more lucrative for exploitation. However, HSES suffers from the drawback of non-linear and disproportionate sugar yields with increased substrate loadings. To overcome this bottleneck, researchers tend to perform HSES at high enzyme loadings. Nonetheless, the production cost of cellulases is one of the key contributors that impair the entire process economics. This review highlights the relentless efforts made globally to attain a high-titre of sugars and their fermentation products by performing efficient HSES at low cellulase loadings. In this context, technical innovations such as advancements in new pretreatment strategies, next-generation cellulase cocktails, additives, accessory enzymes, novel reactor concepts and enzyme recycling studies are especially showcased. The review further covers new insights, learnings and prospects in the area of lignocellulosic bioprocessing.

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A Demonstration of the Consistency of Maize Stover Pretreatment by Soaking in Aqueous Ammonia from Bench to Pilot-Scale

Cited by 3 publications

References 47 publications

A high solids field-to-fuel research pipeline to identify interactions between feedstocks and biofuel production

A high solids field-to-fuel research pipeline to identify interactions between feedstocks and biofuel production

Chemical and thermochemical methods on lignocellulosic biorefinery

Emerging trends in high-solids enzymatic saccharification of lignocellulosic feedstocks for developing an efficient and industrially deployable sugar platform

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