A Delayed Antagonistic Effect of Progesterone on the Estradiol-Induced Differentiation of the Oviductal Epithelium in Spayed Cats

Hg, Verhage; Rm, Brenner

doi:10.1095/biolreprod15.5.654

Cited by 12 publications

(6 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…On the other hand, hormones and drugs known to cause shape changes in cultured bone cells (prostaglandins E x and E 2 , PTH, and (B u ) 2 cAMP) have no effect on the degree of tubulin polymerization (35). With regard to reproductive steroid hormones, estrogen is known to promote differentiation of the ciliated oviduct (36)(37)(38), while progesterone antagonizes the effect of estrogen (39). It is not known whether these hormones affect assembly of the ciliary microtubules and/or synthesis of tubulin and the associated proteins.…”

Section: Discussionmentioning

confidence: 99%

Progesterone Withdrawal Stimulates Mammary Gland Tubulin Polymerization in Pregnant Rats*

Loizzi¹

1985

Endocrinology

View full text Add to dashboard Cite

The size of the polymerized tubulin pool in mammary glands of late pregnant rats increased 2.4-fold 24 h after bilateral ovariectomy using a [3H]colchicine binding assay for tubulin. The total (free plus polymerized) tubulin concentration was not altered. Stimulation of tubulin polymerization as well as the induced rise in lactose content were both completely inhibited in rats that had been fitted with progesterone implants at the time of surgery. The increase in polymerized tubulin was not blocked by 17 beta-estradiol implants, although this treatment did partially inhibit the rise in tissue lactose following ovariectomy. The results indicate that increased polymerization during induced lactogenesis reflects a shift of free tubulin into the polymerized pool and that this shift is related to the withdrawal of progesterone.

show abstract

Section: Discussionmentioning

confidence: 99%

Progesterone Withdrawal Stimulates Mammary Gland Tubulin Polymerization in Pregnant Rats*

Loizzi¹

1985

Endocrinology

View full text Add to dashboard Cite

show abstract

“…Results from numerous studies on normally cycling (Brenner, 1969a,b;Verhage et al, 1973a;Odor et al, 19801, ovariectomized (Brenner, 1969a;Brenner et al, 1974;Verhage and Brenner, 1975;Rumery et al, 1978;Pathek et al, 1979;Odor et al, 19801, fetal and prepubertal animals (Allen, 1928;Flerko, 1954;Brenner, 1969a,b;Verhage et al, 1973b) clearly indicate that estradiol (E2) initiates and is required for the development and subsequent maintenance of a fully functional epithelium, i.e., one composed of columnar ciliated and secretory cells. Progesterone (P), on the other hand, antagonizes the growthpromoting effects of E2 and causes the oviductal epithelium to dedifferentiate (Brenner et al, 1974;Verhage and Brenner, 1976;West et al, 1976West et al, , 1977Bareither and Verhage, 1981). As the epithelium regresses there is a loss of cilia and cessation of secretion.…”

mentioning

confidence: 99%

Effects of progesterone on the oviductal epithelium in estrogen‐primed prepubertal beagles: Light and electron microscopic observations

1984

View full text Add to dashboard Cite

A total of 45 prepubertal beagles 6 to 8 weeks of age were used to study the cytological changes that accompany regression of the oviductal epithelium. The oviductal epithelium in untreated pups consisted of undifferentiated low cuboidal cells that measured 10.3 +/- 2.0 microns in height. In response to estradiol (E2), low cuboidal cells underwent hypertrophy, hyperplasia, and cytodifferentiation and gave rise to columnar ciliated and secretory cells. After 12 days of E2 treatment the epithelium was fully differentiated and measured 29.4 +/- 2.6 microns in height with 56% of the cells possessing cilia. When E2 treatment was continued for an additional 12 days, the epithelium was maintained in a differentiated state. However, if E2 treatment was terminated or progesterone (P) given alone or in conjunction with E2, the oviductal epithelium regressed and after 6 days was composed of low cuboidal cells that ranged in height from 9 to 14 microns with approximately 25% of the cells possessing cilia. A variety of cytological changes characterized the process of regression. The most immediate signs that regression was underway was a reduction in the height of the epithelium and the presence of cells with shrunken, pleomorphic nuclei that lacked prominent nucleoli. Degenerative events included: pinching off and shedding of the apical cytoplasm of cells comprising the epithelium, extrusion of whole cells and/or nuclei, and resorption of cilia and basal bodies. During the first 6 days following E2 withdrawal or P treatment, macrophages and cellular debris were frequently present within the lumen of the oviduct. The process of regression did not proceed synchronously throughout the ampulla of the oviduct, nor did all cells appear to degenerate in the same manner. The cytological changes that accompanied oviductal regression following P treatment were identical to those observed following E2 withdrawal. Results from experiments conducted in the present study show that: E2 induces the oviductal epithelium to differentiate and is required to maintain the epithelium in a differentiated state, E2 withdrawal or P treatment causes the oviductal epithelium to regress, at least three distinct degenerative processes are involved in the transition of columnar ciliated and secretory cells into low cuboidal cells, and regression does not occur synchronously throughout the ampulla region of the oviduct.

show abstract

“…Verhage et al (120) noted that plasma E 2 levels were higher during periods of atrophy and deciliation than they were during periods of hypertrophy and reciliation. They concluded that ciliogenesis is a process that is sensitive to low amounts of E 2 and that serum E 2 levels were probably high enough at all stages of the human menstrual cycle to maintain ciliated cells, but that progesterone, when present, was able to block this estrogen effect and require a recovery phase to follow its withdrawal (142). Similar conclusions were reached in studies on cynomolgus monkeys by West and Brenner (123).…”

Section: A Cyclical Changes In Epithelial Morphologymentioning

confidence: 66%

Endocrine Response in the Fallopian Tube

1984

View full text Add to dashboard Cite

A Delayed Antagonistic Effect of Progesterone on the Estradiol-Induced Differentiation of the Oviductal Epithelium in Spayed Cats

Abstract: We have shown previously (West et al., 1976) that progesterone (P) antagonizes the effect of estradiol (E2) on the oviducts of cats when E2 and P are administered sequentially (E2 first, then

Cited by 12 publications

References 0 publications

Progesterone Withdrawal Stimulates Mammary Gland Tubulin Polymerization in Pregnant Rats*

Progesterone Withdrawal Stimulates Mammary Gland Tubulin Polymerization in Pregnant Rats*

Effects of progesterone on the oviductal epithelium in estrogen‐primed prepubertal beagles: Light and electron microscopic observations

Endocrine Response in the Fallopian Tube

Contact Info

Product

Resources

About