A dehydrogenase-mediated recycling system of NADPH in plant peroxisomes

Corpas, Francisco J.; Barroso, Juan B.; Sandalio, Luisa M.; Distefano, Stefania; Palma, José M.; Lupiáñez, José A.; Rı́o, Luis A. del

doi:10.1042/bj3300777

Cited by 152 publications

(129 citation statements)

References 61 publications

(84 reference statements)

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“…It is worth noting that some of the cofactors involved in the L-Arg-dependent NOS activity in plant peroxisomes have been described in previous studies (Corpas et al, 2009b). Several NADP-dehydrogenases can produce NADPH (Corpas et al, 1998, while the presence of calmodulin in plant peroxisomes has been also demonstrated (Yang and Poovaiah, 2002) being both essential elements for this activity. These results could be important, particularly given that inducible NOS has also been reported in peroxisomes of rat hepatocytes (Stolz et al, 2002;Loughran et al, 2005).…”

Section: Pex12 and Pex13 Appear To Be Involved In The Peroxisomal Impmentioning

confidence: 99%

Peroxisomes Are Required for in Vivo Nitric Oxide Accumulation in the Cytosol following Salinity Stress of Arabidopsis Plants

et al. 2009

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Peroxisomes are unique organelles involved in multiple cellular metabolic pathways. Nitric oxide (NO) is a free radical active in many physiological functions under normal and stress conditions. Using Arabidopsis (Arabidopsis thaliana) wild type and mutants expressing green fluorescent protein through the addition of peroxisomal targeting signal 1 (PTS1), which enables peroxisomes to be visualized in vivo, this study analyzes the temporal and cell distribution of NO during the development of 3-, 5-, 8-, and 11-d-old Arabidopsis seedlings and shows that Arabidopsis peroxisomes accumulate NO in vivo. Pharmacological analyses using nitric oxide synthase (NOS) inhibitors detected the presence of putative calcium-dependent NOS activity. Furthermore, peroxins Pex12 and Pex13 appear to be involved in transporting the putative NOS protein to peroxisomes, since pex12 and pex13 mutants, which are defective in PTS1-and PTS2-dependent protein transport to peroxisomes, registered lower NO content. Additionally, we show that under salinity stress (100 mM NaCl), peroxisomes are required for NO accumulation in the cytosol, thereby participating in the generation of peroxynitrite (ONOO 2 ) and in increasing protein tyrosine nitration, which is a marker of nitrosative stress.

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Section: Pex12 and Pex13 Appear To Be Involved In The Peroxisomal Impmentioning

confidence: 99%

Peroxisomes Are Required for in Vivo Nitric Oxide Accumulation in the Cytosol following Salinity Stress of Arabidopsis Plants

et al. 2009

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“…The C-terminal 10-amino acid residues of 6PGDH, however, directed EYFP to punctate subcellular structures that moved along cytoplasmic strands and coincided with peroxisomes ( Figures 4N1 and 4N2). Although not identified within the proteome analysis, subcellular targeting of 6P-gluconolactonase (6PGL), the precedent enzyme of 6PGDH in the peroxisomal OPPP (Corpas et al, 1998(Corpas et al, , 1999, was also investigated using one specific isoform (At5g24400) out of five Arabidopsis homologs. This enzyme carries both a predicted N-terminal transit peptide and a conserved PTS1 (SKL>; Reumann et al, 2004).…”

Section: Experimental Verification Of Predicted Ptssmentioning

confidence: 99%

Proteome Analysis ofArabidopsisLeaf Peroxisomes Reveals Novel Targeting Peptides, Metabolic Pathways, and Defense Mechanisms

et al. 2007

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We have established a protocol for the isolation of highly purified peroxisomes from mature Arabidopsis thaliana leaves and analyzed the proteome by complementary gel-based and gel-free approaches. Seventy-eight nonredundant proteins were identified, of which 42 novel proteins had previously not been associated with plant peroxisomes. Seventeen novel proteins carried predicted peroxisomal targeting signals (PTS) type 1 or type 2; 11 proteins contained PTS-related peptides. Peroxisome targeting was supported for many novel proteins by in silico analyses and confirmed for 11 representative fulllength fusion proteins by fluorescence microscopy. The targeting function of predicted and unpredicted signals was investigated and SSL>, SSI>, and ASL> were established as novel functional PTS1 peptides. In contrast with the generally accepted confinement of PTS2 peptides to the N-terminal domain, the bifunctional transthyretin-like protein was demonstrated to carry internally a functional PTS2. The novel enzymes include numerous enoyl-CoA hydratases, short-chain dehydrogenases, and several enzymes involved in NADP and glutathione metabolism. Seven proteins, including b-glucosidases and myrosinases, support the currently emerging evidence for an important role of leaf peroxisomes in defense against pathogens and herbivores. The data provide new insights into the biology of plant peroxisomes and improve the prediction accuracy of peroxisome-targeted proteins from genome sequences.

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“…A characteristic property of these oxidative organelles is their metabolic plasticity since their enzymatic content can vary depending on the organism, cell/ tissue-type, and environmental conditions (13,14). In higher plants, peroxisomes contain a complex battery of antioxidative enzymes such as catalase, superoxide dismutase (15,16), the components of the ascorbate-glutathione cycle (17), and the NADP-dehydrogenases of the pentose-phosphate pathway (18). Likewise, the generation of superoxide radicals has also been reported in the matrices and membranes of peroxisomes (19 -21).…”

Section: Nitric Oxide (No)mentioning

confidence: 99%