A dehydrin gene in <i>Physcomitrella patens</i> is required for salt and osmotic stress tolerance

Saavedra, Laura; Svensson, Jan T.; Carballo, Valentina; Izmendi, Darwin; Welin, Björn; Vidal, Sabina

doi:10.1111/j.1365-313x.2005.02603.x

Cited by 151 publications

(121 citation statements)

References 57 publications

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“…Several functional studies confirm a role for LEA proteins and dehydrins in drought and osmotic stress tolerance. A knockout mutant of the dehydrin gene PpBHNA in the moss Physcomitrella patens is impaired in its recovery from salt and osmotic stress treatment [81]. Transgenic tobacco plants expressing two foreign LEA genes, BhLEA1 and BhLEA2 from the dicot Boea hygrometrica, showed higher relative water content, increased PSII activity, decreased electrolyte leakage, and increased peroxidase and superoxide dismutase activities during drought stress [82].…”

Section: Sugar Metabolism and Desiccation Tolerancementioning

confidence: 99%

Molecular mechanisms of desiccation tolerance in the resurrection glacial relic Haberlea rhodopensis

Gechev¹,

Benina²,

Obata

et al. 2012

Cell. Mol. Life Sci.

167

262

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Section: Sugar Metabolism and Desiccation Tolerancementioning

confidence: 99%

Molecular mechanisms of desiccation tolerance in the resurrection glacial relic Haberlea rhodopensis

Gechev¹,

Benina²,

Obata

et al. 2012

Cell. Mol. Life Sci.

167

262

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“…The desiccated and the ABA-treated protonema synthesize several new polypeptides and on the basis of cross reactivity, there is strong evidence for the presence of dehydrins Werner and Bopp, 1993). A dehydrin gene in P. patens has been found to exert a protective role against dehydration tolerance (Saavedra et al, 2006). The ABA-and osmotic stressinducible promoter elements from the wheat Em gene are fully functional in the moss P. patens (Knight et al, 1995).…”

Section: Occurrence Of Aba In Bryophytes and Its Role In Desiccation mentioning

confidence: 99%

Hormonal regulation in green plant lineage families

Johri

2008

Physiol Mol Biol Plants

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The patterns of phytohormones distribution, their native function and possible origin of hormonal regulation across the green plant lineages (chlorophytes, charophytes, bryophytes and tracheophytes) are discussed. The five classical phytohormonesauxins, cytokinins, gibberellins (GA), abscisic acid (ABA) and ethylene occur ubiquitously in green plants. They are produced as secondary metabolites by microorganisms. Some of the bacterial species use phytohormones to interact with the plant as a part of their colonization strategy. Phytohormone biosynthetic pathways in plants seem to be of microbial origin and furthermore, the origin of high affinity perception mechanism could have preceded the recruitment of a metabolite as a hormone. The bryophytes represent the earliest land plants which respond to the phytohormones with the exception of gibberellins. The regulation by auxin and ABA may have evolved before the separation of green algal lineage. Auxin enhances rhizoid and caulonemal differentiation while cytokinins enhance shoot bud formation in mosses. Ethylene retards cell division but seems to promote cell elongation. The presence of responses specific to cytokinins and ethylene strongly suggest the origin of their regulation in bryophytes. The hormonal role of GAs could have evolved in some of the ferns where antheridiogens (compounds related to GAs) and GAs themselves regulate the formation of antheridia.During migration of life forms to land, the tolerance to desiccation may have evolved and is now observed in some of the microorganisms, animals and plants. Besides plants, sequences coding for late embryogenesis abundant-like proteins occur in the genomes of other anhydrobiotic species of microorganisms and nematodes. ABA acts as a stress signal and increases rapidly upon desiccation or in response to some of the abiotic stresses in green plants. As the salt stress also increases ABA release in the culture medium of cyanobacterium Trichormus variabilis, the recruitment of ABA in the regulation of stress responses could have been derived from prokaryotes and present at the level of common ancestor of green plants. The overall hormonal action mechanisms in mosses are remarkably similar to that of the higher plants. As plants are thought to be monophyletic in origin, the existence of remarkably similar hormonal mechanisms in the mosses and higher plants, suggests that some of the basic elements of regulation cascade could have also evolved at the level of common ancestor of plants. The networking of various steps in a cascade or the crosstalk between different cascades is variable and reflects the dynamic interaction between a species and its specific environment.

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“…These functions are usually intimately related to the structural characteristics of these proteins, such as high flexibility, structural adaptability, and extended conformational states. IDPs have been shown to have high ion-binding capacity (Heyen et al, 2002;Alsheikh et al, 2003Alsheikh et al, , 2005Saavedra et al, 2006;, to be able to bind membranes (Danyluk et al, 1998;Ismail et al, 1999a;Koag et al, 2003), and to have both RNA and protein chaperone activity (Tompa and Csermely, 2004). In Arabidopsis, approximately 23% of proteins are predicted to be fully disordered (Oldfield et al, 2005).…”

mentioning

confidence: 99%

Chaperone Activity of ERD10 and ERD14, Two Disordered Stress-Related Plant Proteins

et al. 2008

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ERD10 and ERD14 (for early response to dehydration) proteins are members of the dehydrin family that accumulate in response to abiotic environmental stresses, such as high salinity, drought, and low temperature, in Arabidopsis (Arabidopsis thaliana). Whereas these proteins protect cells against the consequences of dehydration, the exact mode(s) of their action remains poorly understood. Here, detailed evidence is provided that ERD10 and ERD14 belong to the family of intrinsically disordered proteins, and it is shown in various assays that they act as chaperones in vitro. ERD10 and ERD14 are able to prevent the heat-induced aggregation and/or inactivation of various substrates, such as lysozyme, alcohol dehydrogenase, firefly luciferase, and citrate synthase. It is also demonstrated that ERD10 and ERD14 bind to acidic phospholipid vesicles without significantly affecting membrane fluidity. Membrane binding is strongly influenced by ionic strength. Our results show that these intrinsically disordered proteins have chaperone activity of rather wide substrate specificity and that they interact with phospholipid vesicles through electrostatic forces. We suggest that these findings provide the rationale for the mechanism of how these proteins avert the adverse effects of dehydration stresses.

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A dehydrin gene in Physcomitrella patens is required for salt and osmotic stress tolerance

Cited by 151 publications

References 57 publications

Molecular mechanisms of desiccation tolerance in the resurrection glacial relic Haberlea rhodopensis

Molecular mechanisms of desiccation tolerance in the resurrection glacial relic Haberlea rhodopensis

Hormonal regulation in green plant lineage families

Chaperone Activity of ERD10 and ERD14, Two Disordered Stress-Related Plant Proteins

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