A Defect in β-Oxidation Causes Abnormal Inflorescence Development in Arabidopsis

Richmond, Todd; Bleecker, Anthony B.

doi:10.1105/tpc.11.10.1911

Cited by 114 publications

(126 citation statements)

References 54 publications

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“…Similar to the other three Arabidopsis ACXs (SACX, ACX1, and ACX2) (Hayashi et al, 1999;Hooks et al, 1999), AtACX3 transcripts were expressed at moderately high levels in flowers. The requirement of ␤-oxidation in flower development has been demonstrated in Arabidopsis (Richmond and Bleecker, 1999). A mutation in one of the genes encoding a multifunction protein (aim 1, abnormal inflorescence meristem 1), an enzyme that catalyzes the second step in ␤-oxidation, resulted in a range of phenotypes.…”

Section: Q)(l/a) Present At the N-terminal Domainmentioning

confidence: 99%

ACX3, a Novel Medium-Chain Acyl-Coenzyme A Oxidase from Arabidopsis

Froman¹,

Edwards²,

Bursch³

et al. 2000

Plant Physiology

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In a database search for homologs of acyl-coenzyme A oxidases (ACX) in Arabidopsis, we identified a partial genomic sequence encoding an apparently novel member of this gene family. Using this sequence information we then isolated the corresponding full-length cDNA from etiolated Arabidopsis cotyledons and have characterized the encoded recombinant protein. The polypeptide contains 675 amino acids. The 34 residues at the amino terminus have sequence similarity to the peroxisomal targeting signal 2 of glyoxysomal proteins, including the R-[I/Q/L]-X5-HL-XL-X15-22-C consensus sequence, suggesting a possible microsomal localization. Affinity purification of the encoded recombinant protein expressed in Escherichia coli followed by enzymatic assay, showed that this enzyme is active on C8:0-to C14:0-coenzyme A with maximal activity on C12:0-coenzyme A, indicating that it has medium-chain-specific activity. These data indicate that the protein reported here is different from previously characterized classes of ACX1, ACX2, and short-chain ACX (SACX), both in sequence and substrate chain-length specificity profile. We therefore, designate this new gene AtACX3. The temporal and spatial expression patterns of AtACX3 during development and in various tissues were similar to those of the AtSACX and other genes expressed in glyoxysomes. Currently available database information indicates that AtACX3 is present as a single copy gene.

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Section: Q)(l/a) Present At the N-terminal Domainmentioning

confidence: 99%

ACX3, a Novel Medium-Chain Acyl-Coenzyme A Oxidase from Arabidopsis

Froman¹,

Edwards²,

Bursch³

et al. 2000

Plant Physiology

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show abstract

“…This may represent a compensation mechanism, whereby reduced seed set in individual siliques is offset by increased total silique production. Of the known b-oxidation mutants, only abnormal inflorescence meristem 1 (aim1), which is deficient in a MFP, has a reported floral/fertility phenotype (Richmond and Bleecker, 1999). However, compared to aim1 mutants, which exhibit severe defects in floral development and are practically sterile, the cts mutant has a much more subtle reproductive phenotype.…”

Section: Discussion Cts Mutants Have a Subtle Vegetative And Floral Pmentioning

confidence: 99%

“…However, compared to aim1 mutants, which exhibit severe defects in floral development and are practically sterile, the cts mutant has a much more subtle reproductive phenotype. Unlike aim1 (Richmond and Bleecker, 1999), floral morphology was normal in cts mutants, although they exhibited delayed filament extension (Fig. 1B), a phenotype that is shared by plants lacking functional allene oxide synthase and COI1, which are required for JA biosynthesis and perception, respectively (Feys et al, 1994;Park et al, 2002;von Malek et al, 2002).…”

Section: Discussion Cts Mutants Have a Subtle Vegetative And Floral Pmentioning

confidence: 99%

The COMATOSE ATP-Binding Cassette Transporter Is Required for Full Fertility in Arabidopsis

et al. 2007

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COMATOSE (CTS) encodes a peroxisomal ATP-binding cassette transporter required not only for b-oxidation of storage lipids during germination and establishment, but also for biosynthesis of jasmonic acid and conversion of indole butyric acid to indole acetic acid. cts mutants exhibited reduced fertilization, which was rescued by genetic complementation, but not by exogenous application of jasmonic acid or indole acetic acid. Reduced fertilization was also observed in thiolase (kat2-1) and peroxisomal acyl-Coenzyme A synthetase mutants (lacs6-1,lacs7-1), indicating a general role for b-oxidation in fertility. Genetic analysis revealed reduced male transmission of cts alleles and both cts pollen germination and tube growth in vitro were impaired in the absence of an exogenous carbon source. Aniline blue staining of pollinated pistils demonstrated that pollen tube growth was affected only when both parents bore the cts mutation, indicating that expression of CTS in either male or female tissues was sufficient to support pollen tube growth in vivo. Accordingly, abundant peroxisomes were detected in a range of maternal tissues. Although g-aminobutyric acid levels were reduced in flowers of cts mutants, they were unchanged in kat2-1, suggesting that alterations in g-aminobutyric acid catabolism do not contribute to the reduced fertility phenotype through altered pollen tube targeting. Taken together, our data support an important role for b-oxidation in fertility in Arabidopsis (Arabidopsis thaliana) and suggest that this pathway could play a role in the mobilization of lipids in both pollen and female tissues.

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“…One cluster and 7 singles (10 ESTs in total) were related to specific flower genes (Table 2). Abnormal inflorescence meristem 1 (AIM1) could affect inflorescence and floral development in Arabidopsis (Richmond & Bleecker 1999). MADS box protein genes were expressed in different organs and mainly during floral development.…”

Section: Annotation and Classification Of The Subtractive Estsmentioning

confidence: 99%

Analysis of Organ-specific, Expressed Genes in Oncidium Orchid by Subtractive Expressed Sequence Tags Library

2005

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The pseudobulb of Oncidium orchid plays a key role in water, carbohydrate, and other nutrition support during floral development, yet a large scale of gene expression analysis involved in the metabolisms have not been evaluated. By subtracting RsaI-digested cDNAs of leaf from those of psuedobulb, an efficient subtractive cDNA library was developed. In total, 1080 subtractive expressed sequence tags (ESTs) were obtained. Analysis revealed approximately 636 unique gene parts, 120 clusters and 516 singles. Of these sequences, 74.8% were annotated on the database of NCBI GenBank. Peroxidase, sodium/dicarboxylate cotransporter, and mannose-binding lectin were highly expressed. Some gene profiles were identified as related to carbohydrate metabolism involved in mannan, pectin, starch and sucrose biosynthesis. A large fraction of the ESTs (35%) were classified into transportation, stress-related, cell cycle, or regulatory functions. Most genes that were differentially expressed are important in early flowering development, carbohydrate metabolism and stress-response physiology. This efficient organ-specific EST library represented an explicit transcriptome profile of Oncidium pseudobulb.

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A Defect in β-Oxidation Causes Abnormal Inflorescence Development in Arabidopsis

Cited by 114 publications

References 54 publications

ACX3, a Novel Medium-Chain Acyl-Coenzyme A Oxidase from Arabidopsis

ACX3, a Novel Medium-Chain Acyl-Coenzyme A Oxidase from Arabidopsis

The COMATOSE ATP-Binding Cassette Transporter Is Required for Full Fertility in Arabidopsis

Analysis of Organ-specific, Expressed Genes in Oncidium Orchid by Subtractive Expressed Sequence Tags Library

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